A simple HPLC–UV method for the determination of ciprofloxacin in human plasma
Abstract
A rapid and sensitive HPLC-UV method for the determination of ciprofloxacin in human plasma is described. Protein precipitation with acetonitrile was used to separate the drug from plasma protein. An ACE(®) 5 C18 column (250 mm×4.6 mm, 5 μm) with an isocratic mobile phase consisting of phosphate buffer (pH 2.7) and acetonitrile (77:23, v/v) was used for separation. The UV detector was set at 277 nm. The method was validated in the linear range of 0.05-8 μg/ml with acceptable inter- and intra-assay precision, accuracy and stability. The method is simple and rapid and can be used to quantify this widely used antibiotic in the plasma of patients suffering from Peripheral Arterial Disease.
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These reported HPLC assays have extensive chromatographic run times.
“These assays have some disadvantages in terms of extensive chromatographic run times [9,11-13,15,16] a large consumption of non environmentally friendly solvents [11] or a large amount of sample [11,15,16].”
Several HPLC assays with ultraviolet detection for the determination of these drugs, alone or in combination, have been reported.
“Several HPLC assays with ultraviolet (UV) detection for the determination of these drugs, alone or in combination, have been reported [9-17].”
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Ferrone, V., Cotellese, R., Carlucci, M., Di Marco, L., & Carlucci, G. (2018) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2017.12.039 · Cited by 43 · see citing works
- Authors
- Vella, J., Busuttil, F., Bartolo, N. S., Sammut, C., Ferrito, V., Serracino-Inglott, A., Azzopardi, L. M., & LaFerla, G.
- Affiliations
- University of Malta (MT)
- Mater Dei Hospital (MT)
- Corresponding author
- Busuttil, Francesca
- Venue
- Journal of Chromatography B
- Publisher
- Elsevier BV
- Published
- 2015-05
- Volume
- 989
- Pages
- 80-85
- ISSN
- 1570-0232 (ISSN-L)
- OpenAlex
- W2051042605
- Cited by
- 126
- References
- 0
- Citations by year
- 2026: 4 · 2025: 11 · 2024: 18 · 2023: 16 · 2022: 18 · 2021: 16 · 2020: 13 · 2019: 9 · 2018: 5 · 2017: 6 · 2016: 8 · 2015: 2
- FWCI
- 5.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography B (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Health Sciences › Medicine › Pharmacology › Antibiotics Pharmacokinetics and Efficacy
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Forensic Toxicology and Drug Analysis
- Keywords
- ciprofloxacin, human plasma, HPLC-UV quantification, protein precipitation, fluoroquinolones, peripheral arterial disease, therapeutic drug monitoring, method validation
- MeSH terms
- Anti-Bacterial Agents, Chromatography, High Pressure Liquid, Ciprofloxacin, Humans, Peripheral Arterial Disease
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