HPLC and TLC methods for analysis of [ 18 F]FDG and its metabolites from biological samples
Abstract
The most used positron emission tomography (PET) tracer, 2-[ 18 F]fluoro-2-deoxy-d-glucose ([ 18 F]FDG), is a glucose analogue that is used to measure tissue glucose consumption. Traditionally, the Sokoloff model is the basis for [ 18 F]FDG modeling. According to this model, [ 18 F]FDG is expected to be trapped in a cell in the form of [ 18 F]FDG-6-phosphate ([ 18 F]FDG-6-P). However, several studies have shown that in tissues, [ 18 F]FDG metabolism goes beyond [ 18 F]FDG-6-P. Our aim was to develop radioHPLC and radioTLC methods for analysis of [ 18 F]FDG metabolites from tissue samples. The radioHPLC method uses a sensitive on-line scintillation detector to detect radioactivity, and the radioTLC method employs digital autoradiography to detect the radioactivity distribution on a TLC plate. The HPLC and TLC methods were developed using enzymatically in vitro-produced metabolites of [ 18 F]FDG as reference standards. For this purpose, three [ 18 F]FDG metabolites were synthesized: [ 18 F]FDG-6-P, [ 18 F]FD-PGL, and [ 18 F]FDG-1,6-P2. The two methods were evaluated by analyzing the [ 18 F]FDG metabolic profile from rodent ex vivo tissue homogenates. The HPLC method with an on-line scintillation detector had a wide linearity in a range of 5Bq-5kBq (LOD 46Bq, LOQ 139Bq) and a good resolution (Rs ≥1.9), and separated [ 18 F]FDG and its metabolites clearly. The TLC method combined with digital autoradiography had a high sensitivity in a wide range of radioactivity (0.1Bq-2kBq, LOD…
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FDG is a radioactive glucose 2-DG analog that does not enter the glycolytic or Krebs cycle metabolic pathways in the brain.
“18 F-fluoro-2-deoxyglucose (2-DG) (also known as FDG) is a radioactive glucose 2-DG analog that does not enter the glycolytic or Krebs cycle metabolic pathways in the brain (124, 397, 504, 552).”
From Blood-Brain Barrier: From Physiology to Disease and Back (2019) · cites this paper as [504]
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- Authors
- Rokka, J., Grönroos, T. J., Viljanen, T., Solin, O., & Haaparanta-Solin, M.
- Affiliations
- University of Turku (FI)
- Åbo Akademi University (FI)
- Corresponding author
- Rokka, Johanna
- Venue
- Journal of Chromatography B
- Publisher
- Elsevier BV
- Published
- 2017-03
- Volume
- 1048
- Pages
- 140-149
- ISSN
- 1570-0232 (ISSN-L)
- OpenAlex
- W2587718664
- Cited by
- 21
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 3 · 2019: 4 · 2018: 4 · 2017: 1
- FWCI
- 1.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography B (publishedVersion)
- PubMed (publishedVersion)
- Åbo Akademi University Research Portal (submittedVersion)
- Funders
- Turku University Hospital (13250)
- Academy of Finland (266891)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Radiopharmaceutical Chemistry and Applications
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Medical Imaging Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Keywords
- 18F-FDG, fluorodeoxyglucose, PET radiotracer, radiometabolites, digital autoradiography, tracer metabolism, tissue homogenates
- MeSH terms
- Animals, Brain, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Liver, Male, Mice, Inbred C57BL, Rats, Sprague-Dawley, Fluorodeoxyglucose F18, Positron-Emission Tomography, Rats, Metabolome, Limit of Detection
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