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Cited by 53Food Nutrient Composition and Product DevelopmentFood composition and propertiesAnalytical chemistry methods development

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Cereal protein analysis via Dumas method: Standardization of a micro-method using the EuroVector Elemental Analyser

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Serrano, S., Rincón, F., García-Olmo, J.·2013·Journal of Cereal Science
DOI 10.1016/j.jcs.2013.04.006
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Abstract

The Dumas method (DM) of protein analysis in foods was standardized using two cereal references in order to identify the main sources of error during analysis; specifically, the study sought to establish which of the apparent critical factors (ACFs) were really significant influencing factors (IF) for the application of the DM to protein analysis in cereals.Using a non-geometric Plackett-Burman experimental design augmented to resolution IV via fold-over by increasing to twenty-four runs, the following eleven ACFs were investigated: sample weight (WS), capsule sealing process (CW), carrier gas flow (PC), purge gas flow (FHe), oxygen volume (O2), oxygen pressure (ΔO2), sample delay time (SD), sample run time (SR), front furnace temperature (FFT), rear furnace temperature (RFT), and GC oven temperatures (GCT).Two IFs were identified for each of the factors RFT and GCT, so an optimization process was carried out, based on a central composite design.Optimized conditions, ensuring enhanced accuracy and repeatability when determining protein content in cereals using the DM, are reported.

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References 32 · Cited by 53

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Metadata

Authors
Serrano, S., Rincón, F., & García-Olmo, J.
Affiliations
University of Córdoba (ES)
Corresponding author
Serrano, S.
ORCID
Serrano, S.
Venue
Journal of Cereal Science
Publisher
Elsevier BV
Published
2013-07
Volume
58
Issue
1
Pages
31-36
ISSN
0733-5210 (ISSN-L)
DOI
10.1016/j.jcs.2013.04.006
OpenAlex
W2024743151
Cited by
53
References
32
Citations by year
2026: 6 · 2025: 13 · 2024: 12 · 2023: 7 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 3 · 2015: 1 · 2014: 1 · 2013: 1
FWCI
1.49
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • Journal of Cereal Science (publishedVersion)
  • Universidad de Córdoba Insitutional Repository (Universidad de Córdoba) (submittedVersion, cc-by-nc-nd)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food Nutrient Composition and Product Development
  • Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
Keywords
Dumas method, protein analysis, method standardization, key factors, Plackett-Burman design, central composite design, method optimization, gas chromatography, repeatability

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CoChat. (2026). Citation check: Cereal protein analysis via Dumas method: Standardization of a micro-method using the EuroVector Elemental Analyser. https://app.cochat.ai/papers/10.1016/j.jcs.2013.04.006
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Serrano, S., Rincón, F., & García-Olmo, J. (2013). Cereal protein analysis via Dumas method: Standardization of a micro-method using the EuroVector Elemental Analyser. Journal of Cereal Science. https://doi.org/10.1016/j.jcs.2013.04.006