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Cited by 89Mesenchymal stem cell researchHematopoietic Stem Cell TransplantationPluripotent Stem Cells Research

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Cryopreservation does not alter main characteristics of Good Manufacturing Process–grade human multipotent mesenchymal stromal cells including immunomodulating potential and lack of malignant transformation

Luetzkendorf, Jana, Nerger, Katrin, Hering, Julian, Moegel, Angelika, Hoffmann, Katrin, Hoefers, Christiane, Mueller-Tidow, Carsten, Mueller, Lutz P.·2015·Cytotherapy
DOI 10.1016/j.jcyt.2014.10.018
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Abstract

Background aims The immunomodulating capacity of multipotent mesenchymal stromal cells (MSCs) qualifies them as a therapeutic tool in several diseases. However, repeated transplantation with products of reproducible characteristics may be required. This could be achieved with cryopreserved aliquots of Good Manufacturing Practice (GMP)-grade MSCs. However, the impact of cryopreservation on the characteristics of GMP-MSCs is ill defined. Methods We produced fresh and cryopreserved MSCs from human donors with a xenogen-free GMP protocol. Immunogenicity and immunomodulating capacity were tested in co-culture with putative recipient-specific peripheral blood mononuclear cells (PBMCs). Risk of malignant transformation was assessed in vitro and in vivo. Results Cryopreservation had no impact on viability and consensus criteria of MSCs. In co-culture with PBMCs, MSCs showed low immunogenicity and suppressed mitogen-stimulated proliferation of PBMC irrespective of cryopreservation. Cytogenetic aberrations were not observed consistently in fresh and cryopreserved products, and no signs of malignant transformation occurred in functional assays. MSC products from an elderly pretreated donor showed reduced functional quality, but imminent failure of functional criteria could be detected by an increased population doubling time in early passages. Discussion This study is the first systematic analysis on cryopreservation of xenogen-free human bone marrow-derived GMP-MSCs. The data support that cryopreservation does not alter the characteristics of the cells and thus may allow the generation of products for serial transplantation. In addition, the protocol allowed early detection of MSC products with low functional capacity.

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Research works printed but not linked: 24

  1. Tse, 2003, Suppression of allogeneic T-cell proliferation by human marrow stromal cells: implications in transplantation, Transplantation, 75, 389, 10.1097/01.TP.0000045055.63901.A9 · DOI
  2. Keyszer, 2011, Treatment of severe progressive systemic sclerosis with transplantation of mesenchymal stromal cells from allogeneic related donors: report of five cases, Arthritis Rheum, 63, 2540, 10.1002/art.30431 · DOI
  3. Wang, 2012, Double allogenic mesenchymal stem cells transplantations could not enhance therapeutic effect compared with single transplantation in systemic lupus erythematosus, Clin Dev Immunol, 2012, 273291, 10.1155/2012/273291 · DOI
  4. Kotobuki, 2005, Viability and osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells, Tissue Eng, 11, 663, 10.1089/ten.2005.11.663 · DOI
  5. Chin, 2010, Cryopreserved mesenchymal stromal cell treatment is safe and feasible for severe dilated ischemic cardiomyopathy, Cytotherapy, 12, 31, 10.3109/14653240903313966 · DOI
  6. Francois, 2012, Cryopreserved mesenchymal stromal cells display impaired immunosuppressive properties as a result of heat-shock response and impaired interferon-gamma licensing, Cytotherapy, 14, 147, 10.3109/14653249.2011.623691 · DOI · Paper page
  7. Moll, 2014, Do cryopreserved mesenchymal stromal cells display impaired immunomodulatory and therapeutic properties?, Stem Cells, 32, 2430, 10.1002/stem.1729 · DOI
  8. Müller, 2006, Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM, Cytotherapy, 8, 437, 10.1080/14653240600920782 · DOI
  9. Lange, 2007, Accelerated and safe expansion of human mesenchymal stromal cells in animal serum-free medium for transplantation and regenerative medicine, J Cell Physiol, 213, 18, 10.1002/jcp.21081 · DOI
  10. Mendicino, 2014, MSC-based product characterization for clinical trials: an FDA perspective, Cell Stem Cell, 14, 141, 10.1016/j.stem.2014.01.013 · DOI
  11. Bernardo, 2013, Mesenchymal stromal cells: sensors and switchers of inflammation, Cell Stem Cell, 13, 392, 10.1016/j.stem.2013.09.006 · DOI
  12. Mueller, 2006, Presence of mesenchymal stem cells in human bone marrow after exposure to chemotherapy: evidence of resistance to apoptosis induction, Stem Cells, 24, 2753, 10.1634/stemcells.2006-0108 · DOI
  13. Dominici, 2006, Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement, Cytotherapy, 8, 315, 10.1080/14653240600855905 · DOI · Paper page
  14. Ginis, 2012, Evaluation of bone marrow-derived mesenchymal stem cells after cryopreservation and hypothermic storage in clinically safe medium, Tissue Eng Part C Methods, 18, 453, 10.1089/ten.tec.2011.0395 · DOI
  15. de Lima Prata, 2012, Cryopreservation of umbilical cord mesenchymal cells in xenofree conditions, Cytotherapy, 14, 694, 10.3109/14653249.2012.677820 · DOI
  16. Wuchter, 2015, Standardization of Good Manufacturing Practice–compliant production of bone marrow-derived human mesenchymal stromal cells for immunotherapeutic applications, Cytotherapy, 17, 128, 10.1016/j.jcyt.2014.04.002 · DOI
  17. Campioni, 2006, Immunophenotypic heterogeneity of bone marrow–derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment, Haematologica, 91, 364
  18. Tarte, 2010, Clinical-grade production of human mesenchymal stromal cells: occurrence of aneuploidy without transformation, Blood, 115, 1549, 10.1182/blood-2009-05-219907 · DOI · Paper page
  19. Mamidi, 2012, Comparative cellular and molecular analyses of pooled bone marrow multipotent mesenchymal stromal cells during continuous passaging and after successive cryopreservation, J Cell Biochem, 113, 3153, 10.1002/jcb.24193 · DOI
  20. Martinello, 2010, Cryopreservation does not affect the stem characteristics of multipotent cells isolated from equine peripheral blood, Tissue Eng Part C Methods, 16, 771, 10.1089/ten.tec.2009.0512 · DOI
  21. Maijenburg, 2012, The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging, Haematologica, 97, 179, 10.3324/haematol.2011.047753 · DOI
  22. Wagner, 2008, Replicative senescence of mesenchymal stem cells: a continuous and organized process, PLoS One, 3, e2213, 10.1371/journal.pone.0002213 · DOI
  23. Sensebé, 2012, Limited acquisition of chromosomal aberrations in human adult mesenchymal stromal cells, Cell Stem Cell, 10, 9, 10.1016/j.stem.2011.12.005 · DOI
  24. Ben-David, 2012, Significant acquisition of chromosomal aberrations in human adult mesenchymal stem cells: response to Sensebé et al, Cell Stem Cell, 10, 10, 10.1016/j.stem.2011.12.007 · DOI

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Metadata

Authors
Luetzkendorf, J., Nerger, K., Hering, J., Moegel, A., Hoffmann, K., Hoefers, C., Mueller-Tidow, C., & Mueller, L. P.
Affiliations
University Hospital in Halle (DE)
Corresponding author
Mueller, Lutz P.
ORCID
Mueller, Lutz P.
Venue
Cytotherapy
Publisher
Elsevier BV
Published
2015-02
Volume
17
Issue
2
Pages
186-198
ISSN
1465-3249 (ISSN-L)
DOI
10.1016/j.jcyt.2014.10.018
OpenAlex
W2011031881
Cited by
89
References
0
Citations by year
2026: 1 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 8 · 2021: 8 · 2020: 6 · 2019: 9 · 2018: 9 · 2017: 11 · 2016: 19 · 2015: 8
FWCI
8.78
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Cytotherapy (publishedVersion)
  • PubMed (publishedVersion)
Funders
Federal State of Saxony-Anhalt (FKZ: 3646A/0907)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
mesenchymal stem cells, cryopreservation, good manufacturing practices, immunomodulation, immunogenicity, malignant transformation, chromosomal abnormalities, bone marrow-derived cells, serial transplantation, population doubling time, cell quality control
MeSH terms
Adult, Aged, Cell Differentiation, Cell Survival, Cell Transformation, Neoplastic, Cells, Cultured, Female, Humans, Leukocytes, Mononuclear, Male, Middle Aged, Quality Control, Cryopreservation, Coculture Techniques, Guideline Adherence, Pluripotent Stem Cells, Cell Proliferation, Young Adult, Immunomodulation, Mesenchymal Stem Cells, Cell- and Tissue-Based Therapy

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CoChat. (2026). Citation check: Cryopreservation does not alter main characteristics of Good Manufacturing Process–grade human multipotent mesenchymal stromal cells including immunomodulating potential and lack of malignant transformation. https://app.cochat.ai/papers/10.1016/j.jcyt.2014.10.018
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Luetzkendorf, J., Nerger, K., Hering, J., Moegel, A., Hoffmann, K., Hoefers, C., Mueller-Tidow, C., & Mueller, L. P. (2015). Cryopreservation does not alter main characteristics of Good Manufacturing Process–grade human multipotent mesenchymal stromal cells including immunomodulating potential and lack of malignant transformation. Cytotherapy. https://doi.org/10.1016/j.jcyt.2014.10.018

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