The effects of NB-UVB on the hair follicle-derived neural crest stem cells differentiating into melanocyte lineage in vitro
Abstract
Background Narrow-band UVB (NB-UVB) is an effective therapeutic option in the treatment of vitiligo. Despite the apparent clinical efficacy, the underlying mechanism of how topical NB-UVB induces repigmentation in vitiligo has not been clearly elucidated. Objectives To investigate the effects of NB-UVB on the maturation of melanocyte lineage differentiated from hair follicle-derived neural crest stem cells (HF-NCSCs) in vitro. Methods HF-NCSCs were isolated from mouse whisker follicles. The isolated cells were multipotent and expressed embryonic NCSC biomarkers. The effects of NB-UVB on development and differentiation of HF-NCSCs were evaluated. We assessed cell viability, melanogenesis and migration of melanocytes derived from HF-NCSCs after NB-UVB radiation. Tyrosinase, Tyrp1, Dct, Kit, Mc1R, Fzd4, NT3R, Ednra, EP1, TGFβR, Sox10, Mitf, Lef1 and Pax3 gene expression was measured by quantitative RT-PCR, while Tyrosinase, Sox10 and Mitf protein expression were measured by Western blot analysis. Cell migration was measured by Boyden chamber transwell assay. Results NB-UVB increased the expression of tyrosinase during melanocytic differentiation from mouse HF-NCSCs, however, NB-UVB inhibited proliferation of melanocytes derived from HF-NCSCs. Mechanistically, increased melanocyte maturation after NB-UVB treatment was resulted from increased expression of several key melanogenic factors, including Sox10, Kit and Mc1R, which play a critical role to promote tyrosinase expression. Furthermore, the migration of the HF-NCSCs-derived melanocytes was downregulated as NB-UVB doses increased. However, the migration of HF-NCSCs was upregulated under 0.4J NB-UVB radiation. Conclusions Those data provide in vitro evidence demonstrating some direct effects of NB-UVB on pigmentation of melanocyte lineage differentiated from HF-NCSCs, and may provide a possible mechanism for the effect of NB-UVB in vitiligo.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Narrow band UVB induce repigmentation in vitiligo by promotion of melanocyte regeneration.
“and by promotion of melanocyte regeneration [121,122].”
From Molecular Mechanisms of UV-Induced Apoptosis and Its Effects on Skin Residential Cells: The Implication in UV-Based Phototherapy (2013) · cites this paper as [121]
Cites
Mechanisms of hair graying: incomplete melanocyte stem cell maintenance in the niche
Nishimura, E. K., Granter, S. R., & Fisher, D. E. (2005) · Science · doi:10.1126/science.1099593 · Cited by 750 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Lee, C.-H., Wu, S.-B., Hong, C.-H., Yu, H.-S., & Wei, Y.-H. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms14036414 · Cited by 271 · see citing works
- Authors
- Dong, D., Jiang, M., Xu, X., Guan, M., Wu, J., Chen, Q., & Xiang, L.
- Affiliations
- Fudan University (CN)
- University of Pennsylvania (US)
- Corresponding author
- Xiang, Leihong
- Venue
- Journal of Dermatological Science
- Publisher
- Elsevier BV
- Published
- 2012-04
- Volume
- 66
- Issue
- 1
- Pages
- 20-28
- ISSN
- 0923-1811 (ISSN-L)
- OpenAlex
- W2063800528
- Cited by
- 36
- References
- 1
- Citations by year
- 2026: 3 · 2024: 3 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 5 · 2017: 3 · 2016: 6 · 2015: 4 · 2014: 5 · 2013: 4
- FWCI
- 1.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Dermatological Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Core Research for Evolutional Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Melanoma and MAPK Pathways
- Keywords
- narrowband UVB, vitiligo, melanocyte differentiation, melanogenesis, tyrosinase, SOX10, KIT, MC1R, melanocyte migration, repigmentation
- MeSH terms
- Animals, Cell Adhesion, Cell Differentiation, Cell Movement, Dose-Response Relationship, Radiation, Melanocytes, Membrane Glycoproteins, Mice, Inbred C57BL, Oxidoreductases, Monophenol Monooxygenase, Ultraviolet Therapy, Transcriptional Activation, Hair Follicle, Proto-Oncogene Proteins c-kit, Cell Lineage, Intramolecular Oxidoreductases, Mice, SOXE Transcription Factors, Neural Stem Cells, In Vitro Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
