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Cited by 19Osteoarthritis Treatment and MechanismsNatural Compounds in Disease TreatmentPharmacological Effects of Medicinal Plants

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Quantitative proteomics reveal the protective effects of EDS against osteoarthritis via attenuating inflammation and modulating immune response

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Hao, Ying, Wu, Yang, Wang, Shanglong, Wang, Chungguo, Qu, Sihao, Li, Li, Yu, Guohua, Liu, Zimin, Zhao, Zhen, Fan, Pengcheng, Zhang, Zengliang, Shi, Yuanyuan·2021·Journal of Ethnopharmacology
DOI 10.1016/j.jep.2021.113780

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Abstract

Ethnopharmacological relevance Epimedium brevicornu Maxim, Dioscorea nipponica Makino, and Salvia miltiorrhiza Bunge formula (EDS) are three traditional Chinese medicines commonly combined and used to treat osteoarthritis (OA). However, the mechanism of its therapeutic effect on OA is still unclear. Aim of the study The aim of this study was to investigate the potential anti osteoarthritis mechanism of EDS in the treatment of OA rats' model by quantitative proteomics. Materials and methods A papain-induced rat OA model was established, and then EDS was intragastrically administered for 28 days. A label-free quantification proteomics was performed to evaluate the holistic efficacy of EDS against OA and identify the possible protein profiles mechanisms. The expression levels of critical changed proteins were validated by RT-qPCR and Western blotting. The effects of EDS were then assessed by evaluating pathologic changes in the affected knee joint and measuring pressure pain threshold, acoustic reflex threshold, angle of joint curvature. Results Proteomics analysis showed that 62 proteins were significantly upregulated and 208 proteins were downregulated in OA group compared to control group. The changed proteins were involved in activation of humoral immunity response, complement cascade activation, leukocyte mediated immunity, acute inflammatory response, endocytosis regulation, and proteolysis regulation. The EDS treatment partially restored the protein profile changes. The protective effects of EDS on pathologic changes in OA rats' knee joint and pain threshold assessment were consisted with the proteomics results. Conclusions The results suggest that EDS exerted synergistic therapeutic efficacies to against OA through suppressing inflammation, modulating the immune system, relieving joint pain, and attenuating cartilage degradation.

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Metadata

Authors
Hao, Y., Wu, Y., Wang, S., Wang, C., Qu, S., Li, L., Yu, G., Liu, Z., Zhao, Z., Fan, P., Zhang, Z., & Shi, Y.
Affiliations
  • Beijing University of Chinese Medicine (CN)
  • Weill Cornell Medicine (US)
  • State Key Laboratory of Proteomics (CN)
  • Inner Mongolia Medical University (CN)
Corresponding author
  • Fan, Pengcheng
  • Zhang, Zengliang
  • Shi, Yuanyuan
ORCID
Venue
Journal of Ethnopharmacology
Publisher
Elsevier BV
Published
2021-05
Volume
271
Pages
113780
ISSN
0378-8741 (ISSN-L)
DOI
10.1016/j.jep.2021.113780
OpenAlex
W3118248742
Cited by
19
References
3
Citations by year
2025: 4 · 2024: 1 · 2023: 8 · 2022: 5 · 2021: 1
FWCI
2.78
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Ethnopharmacology (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
Beijing University of Chinese Medicine
Topics
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
  • Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
  • Health Sciences › Medicine › Pharmacology (medical) › Pharmacological Effects of Medicinal Plants
Keywords
Epimedium brevicornum, Dioscorea nipponica, Salvia miltiorrhiza, osteoarthritis, anti-inflammatory activity, immunomodulation, humoral immunity, complement system, acute inflammatory response, endocytosis, proteolysis, cartilage degeneration, joint pain, knee joint, traditional Chinese medicine
MeSH terms
Animals, Cartilage, Articular, Complement System Proteins, Disease Models, Animal, Drugs, Chinese Herbal, Immunity, Inflammation, Knee Joint, Osteoarthritis, Papain, Ribosomal Proteins, Cytokines, Rats, Wistar, Pain Threshold, Proteome, MAP Kinase Signaling System, Proteomics, Rats

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