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In-vitro study of the effect of Centella asiatica on cholera toxin production and the gene expression level of ctxA gene in Vibrio cholerae isolates
Abstract
Ethnopharmacological relevance Centella asiatica (L.) Urb or Indian pennywort is a plant of ethnopharmacological relevance, commonly called as Brahmi in South India known for its antimicrobial property in gut and for the treatment of other gut ailments. Natural anti-virulence drugs that disarm pathogens by directly targeting virulence factors or the cell viability and are thus preferred over antibiotics as these drugs impose limited selection pressure for resistance development. In this regard, an in-vitro experimental study was conducted to know the effect of extract of Centella asiatica(L.) Urb. on cholera toxin, gene expression and its vibriocidal effect on five standard strains of Vibrio cholerae; IDH03097 (El Tor variant), N16961 (El Tor), O395 (Classical) as well as five clinical strains (Haitian variant). Aim of the study To study the effect of extract of Centella asiatica on Vibrio cholerae. Materials and methods Crude extract was prepared from the leaves and stem part of the plant. The vibriocidal concentration was tested at different concentrations of the extract. The amount of cholera toxin released from the strains before and after exposure to the extract of Centella asiatica to Vibrio cholerae was measured using Bead ELISA. ctxA gene expression in the strains before and after exposure to extract of Centella asiatica was measured using quantitative real time PCR. All the above assays were performed with commercially obtained asiaticoside as well. Results The vibri…
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“These triterpene saponins are also confirmed to play a significant role in improving organ injuries or damage, repressing inflammation and oxidative stress, protecting against bacterial, fungal and parasitic infections, 10 , 37 , 38”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [10]
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“protecting against bacterial, fungal and parasitic infections, 10 , 37 , 38”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [10]
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“An in vitro study by Vasanth et al. illustrates the effect of asiaticoside in reducing the production of cholera toxins in different strains of Vibrio cholerae, showing promising vibriocidal activity”
From Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review (2023) · cites this paper as [10]
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Cited by
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Therapeutic Properties and Pharmacological Activities of Asiaticoside and Madecassoside: A Review
Bandopadhyay, S., Mandal, S., Ghorai, M., Jha, N. K., Kumar, M., Radha, Ghosh, A., Proćków, J., Pérez de la Lastra, J. M., & Dey, A. (2023) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.17635 · Cited by 220
He, Z., Hu, Y., Niu, Z., Zhong, K., Liu, T., Yang, M., Ji, L., & Hu, W. (2023) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2022.115865 · Cited by 93
Metadata
- Authors
- Vasanth, S., Mohanraj, R. S., & Mandal, J.
- Affiliations
- Jawaharlal Institute of Post Graduate Medical Education and Research (IN)
- Corresponding author
- Mandal, Jharna
- Venue
- Journal of Ethnopharmacology
- Publisher
- Elsevier BV
- Published
- 2021-10
- Volume
- 279
- Pages
- 113930
- ISSN
- 0378-8741 (ISSN-L)
- OpenAlex
- W3131900853
- Cited by
- 12
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 4 · 2022: 2
- FWCI
- 1.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Ethnopharmacology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Jawaharlal Institute Of Postgraduate Medical Education and Research
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Vibrio bacteria research studies
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- Centella asiatica, Vibrio cholerae, cholera toxin, anti-virulence drugs, asiaticoside, plant extracts, El Tor, classical strain, virulence factor inhibition, antibiotic alternatives, clinical isolates, qRT-PCR, leaf and stem extracts, antimicrobial activity
- MeSH terms
- Anti-Bacterial Agents, Cholera Toxin, Dose-Response Relationship, Drug, Genes, Bacterial, Plant Extracts, Triterpenes, Vibrio cholerae, Gene Expression Regulation, Bacterial, Centella
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- Vasanth, S., Mohanraj, R. S., & Mandal, J. (2021). In-vitro study of the effect of Centella asiatica on cholera toxin production and the gene expression level of ctxA gene in Vibrio cholerae isolates. Journal of Ethnopharmacology. https://doi.org/10.1016/j.jep.2021.113930
