Foliar uptake and leaf-to-root translocation of nanoplastics with different coating charge in maize plants

Abstract

Foliar uptake of nanoplastics could represent a pathway responsible for pollutant loads in crop plants, thereby posing risks to human health. To evaluate the foliar uptake, leaf-to-root translocation of nanoplastics, as well as the influences of surface charge on the above processes and physiological effects to plants, maize (Zea mays L.) seedlings were foliar exposed to carboxyl-modified polystyrene nanoplastics (PS-COOH) and amino-modified polystyrene nanoplastics (PS-NH2), respectively. Both PS nanoplastics could effectively accumulate on the maize leaves, accompanied by observable particle aggregation. Due to electrostatic attraction to the negatively charged cell wall, positively charged PS-NH2 association with the leaf surfaces was significantly more than negatively charged PS-COOH. The fraction of PS nanoplastics entry into the leaves could efficiently transfer to the vasculature mainly through stomatal opening and move down to the roots through vascular bundle. Meanwhile, the occurrence of aggregation limited the nanoplastic translocation to the roots, especially for PS-NH2 with larger aggregate sizes relative to PS-COOH. Compared with negatively charged PS-COOH, positively charged PS-NH2 treatment had a higher inhibitory effect on photosynthesis and a stronger stimulation to the activity of antioxidant systems. Overall, our findings give a scientific basis for the risk assessment of nanoplastic exposure in air-plant systems.