Biochar effectively inhibits the horizontal transfer of antibiotic resistance genes via transformation
Abstract
The rapid spread of antibiotic resistance genes (ARGs) has posed a risk to human health. Here, the effects of biochar (BC) on the horizontal transfer of ARG-carrying plasmids to Escherichia coli via transformation were systematically investigated. BC could significantly inhibit the transformation of ARGs and the inhibition degree increased with pyrolysis temperature. Rice straw-derived BC showed a stronger inhibitory effect on the transformation of ARGs than that of peanut shell-derived BC from the same pyrolysis temperature. The inhibitory effect of BC from low pyrolysis temperature (300 ℃) was mainly caused by BC dissolutions, while it was mainly attributed to BC solids for high pyrolysis temperature (700 ℃) BC. BC dissolutions could induce intramolecular condensation and even agglomeration of plasmids, hindering their transformation into competent bacteria. The cell membrane permeability was slightly decreased in BC dissolutions, which might also contribute to the inhibitory effect. Plasmid can be adsorbed by BC solids and the adsorption increased with BC pyrolysis temperature. Meanwhile, BC-adsorbed plasmid could hardly be transformed into E. coli. BC solids could also deactivate E. coli and thereby inhibit their uptake of ARGs. These findings provide a way using BC to limit the spread of ARGs in the environment.
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Transformation of ARGs was reduced by up to 90 % with 8 mg mL−1 of the 700 °C rice straw biochar.
“Transformation of ARGs was reduced by up to 90 % with 8 mg mL 1 of the 700 ◦ C rice straw biochar (Fang et al., 2022).”
From Review of emerging contaminants in green stormwater infrastructure: antibiotic resistance genes, microplastics, tire wear particles, PFAS, and temperature (2024) · cites this paper as Fang et al., 2022
Biochar, in addition to reducing the concentration of ARGs in the bulk soil, can inhibit horizontal gene transfer of ARGs and limit the vertical transport of ARGs in soil.
“Biochar, in addition to reducing the concentration of ARGs in the bulk soil, can inhibit HGT of ARGs and limit the vertical transport of ARGs in soil (Fang et al., 2022; Qiu et al., 2021).”
From Review of emerging contaminants in green stormwater infrastructure: antibiotic resistance genes, microplastics, tire wear particles, PFAS, and temperature (2024) · cites this paper as Fang et al., 2022
Cites
Lian, F., & Xing, B. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.7b02528 · Cited by 641 · see citing works
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Biochar Application in Constructed Wetlands for Wastewater Treatment: A Critical Review
Pandey, D., Singh, S. V., Savio, N., Bhutto, J. K., Srivastava, R. K., Yadav, K. K., Sharma, R., Nandipamu, T. M. K., & Sarkar, B. (2025) · Journal of Water Process Engineering · doi:10.1016/j.jwpe.2024.106713 · Cited by 76 · see citing works
Bodus, B., O'Malley, K., Dieter, G., Gunawardana, C., & McDonald, W. (2024) · Science of The Total Environment · doi:10.1016/j.scitotenv.2023.167195 · Cited by 76 · see citing works
- Authors
- Fang, J., Jin, L., Meng, Q., Shan, S., Wang, D., & Lin, D.
- Venue
- Journal of Hazardous Materials
- Publisher
- Elsevier BV
- Published
- 2022-02
- Volume
- 423
- Pages
- 127150
- ISSN
- 0304-3894
- Cited by
- 128
- References
- 1
- Language
- en
- Licence
- Funders
- National Natural Science Foundation of China (21621005, 21976158)
- National Key Research and Development Program of China (2017YFA0207003)
- Key Technology Research and Development Program of Shandong (2020C01017)
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