Higher-order Molecular Packing in Amyloid-like Fibrils Constructed with Linear Arrangements of Hydrophobic and Hydrogen-bonding Side-chains

Saiki, Masatoshi; Honda, Shinya; Kawasaki, Kazunori; Zhou, Deshan; Kaito, Akira; Konakahara, Takeo; Morii, Hisayuki

doi:10.1016/j.jmb.2005.03.022

Higher-order Molecular Packing in Amyloid-like Fibrils Constructed with Linear Arrangements of Hydrophobic and Hydrogen-bonding Side-chains

Various mutants of the protein fragment, barnase module-1 (1-24) were investigated in order to reveal the structural principle of amyloid-like fibrils. By means of circular dichroism spectroscopy, X-ray diffraction, electron microscopy, and thioflavin T binding assay, we found that the molecules …