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Synergism Between Halide Binding and Proton Transport in a CLC-type Exchanger

Accardi, Alessio, Lobet, Séverine, Williams, Carole, Miller, Christopher, Dutzler, Raimund·2006·Journal of Molecular Biology
DOI 10.1016/j.jmb.2006.07.081
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Abstract

The Cl-/H+ exchange-transporter CLC-ec1 mediates stoichiometric transmembrane exchange of two Cl- ions for one proton. A conserved tyrosine residue, Y445, coordinates one of the bound Cl- ions visible in the structure of this protein and is located near the intersection of the Cl- and H+ pathways. Mutants of this tyrosine were scrutinized for effects on the coupled transport of Cl- and H+ determined electrophysiologically and on protein structure determined crystallographically. Despite the strong conservation of Y445 in the CLC family, substitution of F or W at this position preserves wild-type transport behavior. Substitution by A, E, or H, however, produces uncoupled proteins with robust Cl- transport but greatly impaired movement of H+. The obligatory 2 Cl-/1 H+ stoichiometry is thus lost in these mutants. The structures of all the mutants are essentially identical to wild-type, but apparent anion occupancy in the Cl- binding region correlates with functional H+ coupling. In particular, as determined by anomalous diffraction in crystals grown in Br-, an electrophysiologically competent Cl- analogue, the well-coupled transporters show strong Br- electron density at the "inner" and "central" Cl- binding sites. However, in the uncoupled mutants, Br- density is absent at the central site, while still present at the inner site. An additional mutant, Y445L, is intermediate in both functional and structural features. This mutant clearly exchanges H+ for Cl-, but at a reduced H+-…

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Research works printed but not linked: 32

  1. Jentsch, 2005, CLC chloride channels and transporters, Curr. Opin. Neurobiol., 15, 319, 10.1016/j.conb.2005.05.002 · DOI
  2. Miller, 2006, ClC chloride channels viewed through a transporter lens, Nature, 440, 484, 10.1038/nature04713 · DOI
  3. Accardi, 2004, Secondary active transport mediated by a prokaryotic homologue of ClC Cl- channels, Nature, 427, 803, 10.1038/nature02314 · DOI
  4. Nguitragool, 2006, Uncoupling of a CLC Cl−/H+ exchange transporter by polyatomic anions, J. Mol. Biol., 10.1016/j.jmb.2006.07.006 · DOI
  5. Jentsch, 2005, Physiological functions of CLC Cl- channels gleaned from human genetic disease and mouse models, Annu. Rev. Physiol., 67, 779, 10.1146/annurev.physiol.67.032003.153245 · DOI
  6. Iyer, 2002, A biological role for prokaryotic ClC chloride channels, Nature, 419, 715, 10.1038/nature01000 · DOI
  7. Maduke, 1999, High-level expression, functional reconstitution, and quaternary structure of a prokaryotic ClC-type chloride channel, J. Gen. Physiol., 114, 713, 10.1085/jgp.114.5.713 · DOI
  8. Accardi, 2004, Ionic currents mediated by a prokaryotic homologue of CLC Cl− channels, J. Gen. Physiol., 123, 109, 10.1085/jgp.200308935 · DOI
  9. Dutzler, 2002, X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity, Nature, 415, 287, 10.1038/415287a · DOI · Paper page
  10. Dutzler, 2003, Gating the selectivity filter in ClC chloride channels, Science, 300, 108, 10.1126/science.1082708 · DOI · Paper page
  11. Lobet, 2005, Ion binding properties of the CLC chloride selectivity filter, EMBO J., 25, 24, 10.1038/sj.emboj.7600909 · DOI
  12. Lemieux, 2004, The structural basis of substrate translocation by the Escherichia coli glycerol-3-phosphate transporter: a member of the major facilitator superfamily, Curr. Opin. Struct. Biol., 14, 405, 10.1016/j.sbi.2004.06.003 · DOI
  13. Abramson, 2003, The lactose permease of Escherichia coli: overall structure, the sugar-binding site and the alternating access model for transport, FEBS Letters, 555, 96, 10.1016/S0014-5793(03)01087-1 · DOI
  14. Knauf, 2002, Substrate-dependent reversal of anion transport site orientation in the human red blood cell anion-exchange protein, AE1, Proc. Natl Acad. Sci. USA, 99, 10861, 10.1073/pnas.162402399 · DOI
  15. Hunte, 2005, Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH, Nature, 435, 1197, 10.1038/nature03692 · DOI
  16. Abramson, 2003, Structure and mechanism of the lactose permease of Escherichia coli, Science, 301, 610, 10.1126/science.1088196 · DOI
  17. Yin, 2006, Structure of the multidrug transporter EmrD from Escherichia coli, Science, 312, 741, 10.1126/science.1125629 · DOI
  18. Accardi, 2005, Separate ion pathways in a Cl−/H+ exchanger, J. Gen. Physiol., 126, 563, 10.1085/jgp.200509417 · DOI
  19. Bell, 2006, Site-directed fluorescence studies of a prokaryotic ClC antiporter, Biochemistry, 45, 6773, 10.1021/bi0523815 · DOI
  20. Li, 1993, Environments and mechanistic roles of the tyrosine residues of delta 5-3-ketosteroid isomerase, Biochemistry, 32, 1816, 10.1021/bi00058a016 · DOI
  21. Karsten, 2005, A catalytic triad is responsible for acid-base chemistry in the Ascaris suum NAD-malic enzyme, Biochemistry, 44, 3626, 10.1021/bi047826o · DOI
  22. Hwang, 2005, Mechanistic roles of Ser-114, Tyr-155, and Lys-159 in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni, J. Biol. Chem., 280, 3522, 10.1074/jbc.M411751200 · DOI
  23. Nyquist, 2003, Direct observation of protonation reactions during the catalytic cycle of cytochrome c oxidase, Proc. Natl Acad. Sci. USA, 100, 8715, 10.1073/pnas.1530408100 · DOI
  24. Ruiz, 2004, The crystallographic structure of the aldose reductase-IDD552 complex shows direct proton donation from tyrosine 48, Acta Crystallog. sect. D, 60, 1347, 10.1107/S0907444904011370 · DOI
  25. Estevez, 2003, Conservation of chloride channel structure revealed by an inhibitor binding site in ClC-1, Neuron, 38, 47, 10.1016/S0896-6273(03)00168-5 · DOI
  26. Chen, 2003, Side-chain charge effects and conductance determinants in the pore of ClC-0 chloride channels, J. Gen. Physiol., 122, 133, 10.1085/jgp.200308844 · DOI
  27. Accardi, 2003, Conformational changes in the pore of CLC-0, J. Gen. Physiol., 122, 277, 10.1085/jgp.200308834 · DOI
  28. Heginbotham, 1994, Mutations in the K+ channel signature sequence, Biophys. J., 66, 1061, 10.1016/S0006-3495(94)80887-2 · DOI
  29. Otwinowski, 1997, Processing of X-ray diffraction data collected in oscillation mode, Methods Enzymol., 267, 307, 10.1016/S0076-6879(97)76066-X · DOI · Paper page
  30. CCP4, 1994, Collaborative Computational Project No. 4. The CCP4 Suite: Programs for X-ray crystallography, Acta Crystallog. sect. D, 50, 760, 10.1107/S0907444994003112 · DOI
  31. Brunger, 1998, Crystallography and NMR system: a new software suite for macromolecular structure determination, Acta Crystallog. sect. D, 54, 905, 10.1107/S0907444998003254 · DOI
  32. Emsley, 2004, Coot: model-building tools for molecular graphics, Acta Crystallog. sect. D, 60, 2126, 10.1107/S0907444904019158 · DOI · Paper page

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Metadata

Authors
Accardi, A., Lobet, S., Williams, C., Miller, C., & Dutzler, R.
Venue
Journal of Molecular Biology
Publisher
Elsevier BV
Published
2006-09
Volume
362
Issue
4
Pages
691-699
ISSN
0022-2836
DOI
10.1016/j.jmb.2006.07.081
Cited by
106
References
0
Language
en
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