The DOI matches its Crossref record.
Simultaneous determination of nine major active compounds in Dracocephalum rupestre by HPLC
Abstract
A new method was developed for the simultaneous determination of nine major constituents in Dracocephalum rupestre, including 5,7-dihydroxychromone (1), eriodictyol-7-O-beta-d-glucoside (2), luteolin-7-O-beta-d-glucoside (3), naringenin-7-O-beta-d-glucoside (4), apigenin-7-O-beta-d-glucoside (5), eriodictyol (6), luteolin (7), naringenin (8) and apigenin (9). The quantitative determination was conducted by reversed phase high-performance liquid chromatography with photodiode array detector (LC-PDA). Separation was performed on an Agilent Eclipse XDB-C(18) column (150 mm x 4.6 mm i.d., 5 microm) with gradient elution of acetonitrile and 0.5% aqueous acetic acid. The components were identified by retention time, ultraviolet (UV) spectra and quantified by LC-PDA at 260 nm. All calibration curves showed good linearity (r(2)>0.999) within test ranges. The reproducibility was evaluated by intra- and inter-day assays and R.S.D. values were less than 3.0%. The recoveries were between 95.15 and 104.45%. The limits of detection (LOD) ranged from 0.002 to 0.422 microg/ml and limits of quantification (LOQ) ranged from 0.005 to 1.208 microg/ml, respectively. The identity of the peaks was further confirmed by high-performance liquid chromatography with triple-quadrupole mass spectrometry system coupled with electrospray ionization (ESI) interface. The developed method was applied to the determination of nine constituents in 14 samples of D. rupestre collected at various harvesting times. M…
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A water-acetonitrile system was more suitable for separation of luteolin, apigenin and isorhamnetin in the present study as well as in the literature.
“However, wateracetonitrile system was found to be more suitable for the separation of luteolin, apigenin and isorhamnetin in the present study as well as in the literatures [32,33].”
Citations
Cites
No references could be resolved yet.
Research works printed but not linked: 14
- Wu, 1977, vol. 65, no. 2
- Jiangsu New Medical College, Dictionary of Chinese Traditional Medicine, Shanghai Science and Technology Press, Shanghai, 1977, p. 1347.
- Ren, 2005, Chin. Pharm. J., 22, 1695
- Ren, 2007, J. Chromatogr. A, 1161, 334, 10.1016/j.chroma.2007.06.034 · DOI
- Ren, 2008, Phytochemistry, 69, 1425, 10.1016/j.phytochem.2008.01.013 · DOI
- Han, 2008, Eur. J. Pharmacol., 581, 47, 10.1016/j.ejphar.2007.11.048 · DOI
- Vaughn, 1995, J. Chem. Ecol., 21, 107, 10.1007/BF02036645 · DOI
- Areias, 2001, Biochem. Pharmacol., 62, 111, 10.1016/S0006-2952(01)00621-9 · DOI
- Kanno, 2006, Toxicol. Lett., 166, 131, 10.1016/j.toxlet.2006.06.005 · DOI
- Lee, 2001, Biochem. Biophys. Res. Commun., 284, 681, 10.1006/bbrc.2001.5001 · DOI
- Tsuji, 2008, Chem. Biol. Interact., 171, 37, 10.1016/j.cbi.2007.08.007 · DOI
- Chen, 2007, Life Sci., 81, 1602, 10.1016/j.lfs.2007.09.028 · DOI · Paper page
- Shukla, 2008, Free Rad. Biol. Med., 44, 1833, 10.1016/j.freeradbiomed.2008.02.007 · DOI
- Basilea, 1999, Phytochemistry, 52, 1479, 10.1016/S0031-9422(99)00286-1 · DOI
Cited by
Sorted by citations
Wei, Z., Qi, X., Li, T., Luo, M., Wang, W., Zu, Y., & Fu, Y. (2015) · Separation and Purification Technology · doi:10.1016/j.seppur.2015.05.015 · Cited by 274
Metadata
- Authors
- Ren, D.-M., Qu, Z., Wang, X.-N., Shi, J., & Lou, H.-X.
- Affiliations
- Shandong University (CN)
- Venue
- Journal of Pharmaceutical and Biomedical Analysis
- Publisher
- Elsevier BV
- Published
- 2008-12
- Volume
- 48
- Issue
- 5
- Pages
- 1441-1445
- ISSN
- 0731-7085 (ISSN-L)
- OpenAlex
- W2052443826
- Cited by
- 25
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 2 · 2012: 2
- FWCI
- 5.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Pharmaceutical and Biomedical Analysis (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Shandong University
- National Natural Science Foundation of China
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Keywords
- flavonoids, luteolin-7-O-glucoside, apigenin-7-O-glucoside, eriodictyol, luteolin, naringenin, apigenin, simultaneous determination, quality control, harvest time, flavonoid accumulation, HPLC method development
- MeSH terms
- Calibration, Chromatography, High Pressure Liquid, Chromones, Drug Stability, Drugs, Chinese Herbal, Glucosides, Medicine, Chinese Traditional, Plant Extracts, Quality Control, Reference Standards, Seasons, Spectrophotometry, Ultraviolet, Temperature, Time Factors, Reproducibility of Results, Molecular Structure, Spectrometry, Mass, Electrospray Ionization, Plant Components, Aerial, Flavanones, Apigenin, Luteolin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
