Metabonomic study of biochemical changes in the serum of type 2 diabetes mellitus patients after the treatment of metformin hydrochloride
Abstract
A metabonomic study on biochemical changes in the serum of type 2 diabetes mellitus patients after the treatment of metformin hydrochloride was performed. (1)H NMR and UPLC/MS were used to generate metabolic fingerprints for the metabonomic analysis of serum samples obtained from 20 type 2 diabetes mellitus patients without any drugs treatment and 15 type 2 diabetes mellitus patients treated with metformin hydrochloride for 3 months. The resulting data were subjected to chemometric analysis (principal component analysis and partial least squares discriminant analysis) to investigate the effect of metformin hydrochloride on serum metabolite profiles of type 2 diabetes mellitus patients. (1)H NMR spectroscopic analysis revealed increased trimethylamine-N-oxide (TMAO), 3-hydroxybutyrate (3-HB) and decreased glucose, N-acetyl glycoprotein (NAC), lipoprotein, lactate, acetoacetate and unsaturated lipids in serum from metformin treated patients compared to untreated ones. UPLC/MS in positive electrospray ionization detected increased tryptophan and decreased lysophosphatidylcholines (C16:0 LPC, C18:0 LPC and C18:2 LPC) as well as phenylalanine in treated group. Both analytical techniques used in this study were able to detect biochemical changes in the serum of type 2 diabetes mellitus patients after the treatment of metformin hydrochloride, which may be helpful to the understanding of action mechanism of metformin hydrochloride.
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Diabetic patients treated with metformin had decreased glucose but increased plasma TMAO compared with untreated individuals.
“By contrast, patients with diabetes treated with metformin had decreased glucose but increased plasma TMAO concentrations compared with untreated individuals (55).”
From Trimethylamine <i>N</i> -Oxide, the Microbiome, and Heart and Kidney Disease (2017) · cites this paper as [55]
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References 49 · Cited by 104
- Authors
- Huo, T., Cai, S., Lu, X., Sha, Y., Yu, M., & Li, F.
- Affiliations
- Shenyang Pharmaceutical University (CN)
- Corresponding author
- Li, Famei
- Venue
- Journal of Pharmaceutical and Biomedical Analysis
- Publisher
- Elsevier BV
- Published
- 2009-05
- Volume
- 49
- Issue
- 4
- Pages
- 976-982
- ISSN
- 0731-7085 (ISSN-L)
- OpenAlex
- W1963857188
- Cited by
- 104
- References
- 49
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 8 · 2022: 2 · 2021: 6 · 2020: 7 · 2019: 5 · 2018: 3 · 2017: 3 · 2016: 9 · 2015: 7 · 2014: 11 · 2013: 11 · 2012: 8
- FWCI
- 3.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Pharmaceutical and Biomedical Analysis (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education of the People's Republic of China (20060163004)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Keywords
- type 2 diabetes, metformin hydrochloride, serum metabolites, metabolomics, trimethylamine N-oxide, beta-hydroxybutyrate, tryptophan, lysophosphatidylcholine, phenylalanine, mechanism of action, metabolic fingerprint
- MeSH terms
- Chromatography, High Pressure Liquid, Diabetes Mellitus, Type 2, Humans, Hypoglycemic Agents, Metformin, Magnetic Resonance Spectroscopy, Reference Standards, Mass Spectrometry, Reproducibility of Results, Biomarkers, Multivariate Analysis, Least-Squares Analysis, Spectrometry, Mass, Electrospray Ionization, Principal Component Analysis, Metabolomics
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