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Cited by 22Mesenchymal stem cell researchChronic Kidney Disease and DiabetesLiver physiology and pathology

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Therapeutic implications of mesenchymal stem cells transfected with hepatocyte growth factor transplanted in rat kidney with unilateral ureteral obstruction

Liu, Xin, Shen, Wei, Yang, Yi, Liu, Ge·2011·Journal of Pediatric Surgery
DOI 10.1016/j.jpedsurg.2010.09.040
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Abstract

Purpose The purpose of the study was to establish whether bone marrow mesenchymal stem cells (MSCs) transfected with hepatocyte growth factor (HGF) can migrate and localize in the rat's kidney with unilateral ureteral obstruction (UUO) and contribute to repair of renal fibrosis. Methods We separated and cultured bone marrow-derived MSCs of male rats in vitro and transfected them with adenovirus-mediated HGF (Ad-HGF). The expression of HGF was measured with enzyme-linked immunosorbent assay. Sixty female rats were sham operated (n = 24) or subjected to left UUO: Ad-HGF-transfected MSCs, uninfected MSCs, or saline was injected into the rat's tail vein. Kidney tissue was collected at the end of the seventh or 14th day after operation. The distribution of Y chromosome in the kidney after Ad-HGF-transfected MSCs transplantation was determined by an in situ hybridization method. As the hallmark of myofibroblasts, α-smooth muscle actin (expression of which significantly increases in the presence of renal fibrosis) was detected by immunohistochemistry in all UUO rats' left kidney tissue. Results Y chromosome-positive cells were found only in the obstructed kidney of the transplantation group. The positive cells were mainly distributed in the tubular cells. The average intensity of immunolabeling for α-smooth muscle actin in the transplanted group significantly decreased compared with sham-transplanted group (P < .05), and the expression in the rats injected with uninfected MSCs was higher than that in the rats with MSCs transfected with HGF (P < .05). Conclusions Mesenchymal stem cells transfected with HGF can migrate to the rat kidney with UUO and are mainly distributed in the region of renal tubular epithelial cells. The data indicate that MSCs transfected with HGF contribute to a reduction of renal fibrosis after ureteral obstruction and suggest that this may be exploited therapeutically.

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Metadata

Authors
Liu, X., Shen, W., Yang, Y., & Liu, G.
Affiliations
Sheng Jing Hospital (CN)
ORCID
Venue
Journal of Pediatric Surgery
Publisher
Elsevier BV
Published
2011-03
Volume
46
Issue
3
Pages
537-545
ISSN
0022-3468 (ISSN-L)
DOI
10.1016/j.jpedsurg.2010.09.040
OpenAlex
W1989000443
Cited by
22
References
1
Citations by year
2026: 1 · 2024: 2 · 2022: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 2
FWCI
1.45
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Pediatric Surgery (publishedVersion)
  • PubMed (publishedVersion)
Funders
Academy of Medical Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
  • Health Sciences › Medicine › Nephrology › Chronic Kidney Disease and Diabetes
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Keywords
mesenchymal stem cells, hepatocyte growth factor, adenoviral gene transfer, unilateral ureteral obstruction, renal fibrosis, stem cell transplantation, alpha-smooth muscle actin, myofibroblasts, bone marrow-derived stem cells, renal tubular epithelium
MeSH terms
Adenoviridae, Animals, Disease Models, Animal, Female, Fibrosis, Genetic Vectors, Hydronephrosis, Kidney, Male, Random Allocation, Recombinant Fusion Proteins, Transduction, Genetic, Ureteral Obstruction, Genetic Therapy, Rats, Sprague-Dawley, Hepatocyte Growth Factor, Mesenchymal Stem Cell Transplantation, Rats, Mesenchymal Stem Cells

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