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Cited by 32Biocrusts and Microbial EcologyPolar Research and EcologyLight effects on plants

Hyperspectral imaging of snow algae and green algae from aeroterrestrial habitats

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Holzinger, Andreas, Allen, Michael C., Deheyn, Dimitri D.·2016·Journal of Photochemistry and Photobiology B: Biology
DOI 10.1016/j.jphotobiol.2016.07.001PMC PMC5061078
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Abstract

Snow algae and green algae living in aeroterrestrial habitats are ideal objects to study adaptation to high light irradiation. Here, we used a detailed description of the spectral properties as a proxy for photo-acclimation/protection in snow algae (Chlamydomonas nivalis, Chlainomonas sp. and Chloromonas sp.) and charophyte green algae (Zygnema sp., Zygogonium ericetorum and Klebsormidium crenulatum). The hyperspectral microscopic mapping and imaging technique allowed us to acquire total absorption spectra of these microalgae in the waveband of 400-900nm. Particularly in Chlamydomonas nivalis and Chlainomonas sp., a high absorbance between 400-550nm was observed, due to naturally occurring secondary carotenoids; in Chloromonas sp. and in the charopyhte algae this high absorbance was missing, the latter being close relatives to land plants. To investigate if cellular water loss has an influence on the spectral properties, the cells were plasmolysed in sorbitol or desiccated at ambient air. While in snow algae, these treatments did hardly change the spectral properties, in the charopyhte algae the condensation of the cytoplasm and plastids increased the absorbance in the lower waveband of 400-500nm. These changes might be ecologically relevant and photoprotective, as aeroterrestrial algae are naturally exposed to occasional water limitation, leading to desiccation, which are conditions usually occurring together with higher irradiation.

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  • Spectra predicted from Monte Carlo transfer matrix simulations based on cryo-SEM morphometric data closely match empirical reflectance spectra recorded for individual cells by hyperspectral imaging.

    “we found close matches between spectra predicted from simulations based on a Monte Carlo transfer matrix33 (with morphometic data derived from cryo-SEM) and empirical reflectance spectra recorded for individual cells by hyperspectral imaging microscopy34 (Fig. 3f, g)”

    From In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning (2020) · cites this paper as [34]

    The Introduction and Discussion describe hyperspectral microscopy of individual algal cells, supporting the citation as an example of that method. But the Introduction explicitly says this study used absorbance rather than reflectance; the paper does not establish the citing authors’ reported match between simulated and empirical spectra. (LLM, conf=0.88)

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Research works printed but not linked: 41

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Cited by

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Authors
Holzinger, A., Allen, M. C., & Deheyn, D. D.
Affiliations
  • Universität Innsbruck (AT)
  • Scripps Institution of Oceanography (US)
Corresponding author
  • Holzinger, Andreas
  • Deheyn, Dimitri D.
ORCID
Venue
Journal of Photochemistry and Photobiology B: Biology
Publisher
Elsevier BV
Published
2016-09
Volume
162
Pages
412-420
ISSN
1011-1344 (ISSN-L)
DOI
10.1016/j.jphotobiol.2016.07.001
PMC
PMC5061078
OpenAlex
W2473777388
Cited by
32
References
0
Citations by year
2026: 3 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 5 · 2020: 3 · 2019: 5 · 2018: 5 · 2017: 2
FWCI
4.36
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Photochemistry and Photobiology B Biology (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • University of Innsbruck
  • FWF) (P 24242-B16, I 1951-B16)
  • Air Force Office of Scientific Research (FA9550-10-1-0555)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Biocrusts and Microbial Ecology
  • Physical Sciences › Environmental Science › Ecology › Polar Research and Ecology
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
Keywords
snow algae, green algae, hyperspectral imaging, Chlamydomonas nivalis, Chloromonas, Zygnema, secondary carotenoids, absorption spectra, photoprotection, light acclimation, desiccation, plasmolysis, water limitation, charophytes
MeSH terms
Chlamydomonas, Desiccation, Ecosystem, Molecular Imaging

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