IgG is transported across the mouse yolk sac independently of FcγRIIb
Open accessAbstract
While generally accepted that FcRn of the human syncytiotrophoblast and the mouse yolk sac endoderm is the major IgG transporter, the finding of a different Fc receptor FcgammaRIIb (RIIb) in the human placental endothelium has suggested the existence of an additional IgG transporter. Testing our hypothesis in mouse, we found that while RIIb is expressed in the yolk sac vasculature, IgG concentrations in fetuses of wild-type mice (RIIb(+/+)) and mice with a null mutation in the gene encoding RIIb (RIIb(-/-) mice) are not different, and we thus reject our hypothesis that yolk sac RIIb transports IgG in utero in the mouse. However, the capillary bed in the mouse yolk sac is structurally more complex than in human placenta, consisting of three types of cells: an RIIb-negative endothelium, a unique RIIb-bearing cell that also expresses 2 out of 4 macrophage markers but not endothelial cell or pericyte markers, and pericytes. As in the human placenta the b2 isoform of RIIb predominates in the mouse yolk sac. Remarkably only a single capillary channel rather than 2 channels with a loop is found in each yolk sac villus, which, along with intracapillary erythrocytes, suggests that blood flow is peristaltic, mediated by pericytes. It is not clear whether RIIb in the human placental villus might mediate an IgG transport function in light of the mouse yolk sac equivalent failing to do so.
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Mouse and rat models differ from humans in key features including levels of FcRn expression, immunological function, and placental anatomy.
“However, they differ from humans in many key features including levels of FcRn expression (132), immunological function (133), and placental anatomy (77).”
From Factors Affecting the FcRn-Mediated Transplacental Transfer of Antibodies and Implications for Vaccination in Pregnancy (2017) · cites this paper as [77]
The Introduction and Discussion describe differences between mouse yolk-sac placental anatomy and human placental anatomy, supporting the part of the sentence to which this citation is attached. These passages do not establish the corresponding anatomical difference for rats. The separate claims about FcRn expression levels and immunological function are attached to other citations. (LLM, conf=0.85)
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Chaudhury, C., Mehnaz, S., Robinson, J. M., Hayton, W. L., Pearl, D. K., Roopenian, D. C., & Anderson, C. L. (2003) · The Journal of Experimental Medicine · doi:10.1084/jem.20021829 · Cited by 640 · see citing works
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Research works printed but not linked: 30
- Anderson CL Chaudhury C Kim J Bronson CL Wani MA Mohanty S 2006 Perspective: FcRn transports albumin: relevance to immunology and medicine Trends Immunol 27 343 348 16731041
- Burmeister WP Gastinel LN Simister NE Blum ML Bjorkman PJ 1994 Crystal structure at 2.2 Å resolution of the MHC-related neonatal Fc receptor Nature 372 336 343 7969491
- Cattelino A Liebner S Gallini R Zanetti A Balconi G Corsi A Bianco P Wolburg H Moore R Oreda B Kemler R Dejana E 2003 The conditional inactivation of the beta-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility J Cell Biol 162 1111 1122 12975353 PMC2172846
- Chaudhury C Kim J Mehnaz S Wani MA Oberyszyn TM Hayton WL Robinson JM Anderson CL 2006 Accelerated transferrin degradation in HFE-deficient mice is due to increased transferrin saturation J Nutr 136 1 6 17116709
- Christensen EI Verroust PJ 2002 Megalin and cubilin, role in proximal tubule function and during development Pediatr Nephrol 17 993 999 12478347
- Daeron M 1997 Fc receptor biology Annu Rev Immunol 15 203 234 9143687
- Fahey JL Robinson AG 1963 Factors controlling serum gammaglobulin concentration J Exp Med 118 845 868 14087625 PMC2137682
- Gavrilin MA Bouakl IJ Knatz NL Duncan MD Hall MW Gunn JS Wewers MD 2006 Internalization and phagosome escape required for Francisella to induce human monocyte IL-1beta processing and release Proc Natl Acad Sci U S A 103 141 146 16373510 PMC1324976
- Ghetie V Ward ES 2000 Multiple roles for the major histocompatibility complex Class I-related receptor FcRn Annu Rev Immunol 18 739 766 10837074
- Hogarth M Hulett MD Osman N 1992 Fc gamma receptors: gene structure and receptor function Immunologic research 11 217 225 1287117
- Holness CL da Silva RP Fawcett J Gordon S Simmons DL 1993 Macrosialin, a mouse macrophage-restricted glycoprotein, is a member of the lamp/lgp family J Biol Chem 268 9661 9666 8486654
- Israel EJ Patel VK Taylor SF Marshak-Rothstein A Simister NE 1995 Requirement for a b2-microglobulin-associated Fc receptor for acquisition of maternal IgG by fetal and neonatal mice J Immunol 154 6246 6251 7759862
- Kim J Bronson CL Hayton WL Radmacher MD Roopenian DC Robinson JM Anderson CL 2006 Albumin turnover: FcRn-mediated recycling saves as much albumin from degradation as the liver produces Am J Physiol Gastrointest Liver Physiol 290 G352 G360 16210471
- Kim J Mohanty S Ganesan LP Keding H Jarjoura D Hayton WL Robinson JM Anderson CL 2009 FcRn in the yolk sac endoderm of mouse is required for IgG transport to fetus J Immunol 182 2583 2589 19234152 PMC2676880
- Leach JL Sedmak DD Osborne JM Rahill B Lairmore MD Anderson CL 1996 Isolation from human placenta of the IgG transporter, FcRn, and localization to the syncytiotrophoblast: Implications for maternal-fetal antibody transport J Immunol 157 3317 3322 8871627
- Li Z Takakura N Oike Y Imanaka T Araki K Suda T Kaname T Kondo T Abe K Yamamura K 2003 Defective smooth muscle development in qkI-deficient mice Dev Growth Differ 45 449 462 14706070
- Lichanska AM Hume DA 2000 Origins and functions of phagocytes in the embryo Exp Hematol 28 601 611 10880746
- Lyden TW Anderson CL Robinson JM 2002 The endothelium but not the syncytiotrophoblast of human placenta expresses caveolae Placenta 23 640 652 12361683
- Lyden TW Robinson JM Tridandapani S Teillaud JL Garber SA Osborne JM Frey J Budde P Anderson CL 2001 The Fc receptor for IgG expressed in the villus endothelium of human placenta is FcγRIIb2 J Immunol 166 3882 3889 11238632
- Miettinen HM Matter K Hunziker W Rose JK Mellman I 1992 Fc receptor endocytosis is controlled by a cytoplasmic domain determinant that actively prevents coated pit localization J Cell Biol 116 875 888 1734021 PMC2289334
- Miettinen HM Rose JK Mellman I 1989 Fc receptor isoforms exhibit distinct abilities for coated pit localization as a result of cytoplasmic domain heterogeneity Cell 58 317 327 2568890
- Mohanty S Anderson CL Robinson JM 2009 The expression of caveolin-1 and the distribution of caveolae in the murine placenta and yolk sac: Parallels to the human placenta Submitted 20004972
- Morris L Graham CF Gordon S 1991 Macrophages in haemopoietic and other tissues of the developing mouse detected by the monoclonal antibody F4/80 Development 112 517 526 1794320
- Mousavi SA Sporstol M Fladeby C Kjeken R Barois N Berg T 2007 Receptor-Mediated Endocytosis of Immune Complexes in Rat Liver Sinusoidal Endothelial Cells Is Mediated by FcRIIb2 Hepatology 46 871 884 17680646
- Nakamura K Cambier JC 1998 B cell antigen receptor (BCR)-mediated formation of a SHP-2-pp120 complex and its inhibition by Fc gamma RIIB1-BCR coligation J Immunol 161 684 691 9670943
- Ozerdem U Grako KA Dahlin-Huppe K Monosov E Stallcup WB 2001 NG2 proteoglycan is expressed exclusively by mural cells during vascular morphogenesis Dev Dyn 222 218 227 11668599
- Rabe-Hesketh S Skrondal A 2005 Multilevel and Longitudinal Modeling Using Stata Stata Press College Station Texas
- Takizawa T Anderson CL Robinson JM 2005 A novel FcγRII receptor-defined, IgG-containing, compartment in placental endothelium J Immunol 175 2331 2339 16081803
- Van Den Herik-Oudijk IE Westerdaal NAC Henriquez NV Capel PJA van de Winkel JGJ 1994 Functional analysis of human FcγRII (CD32) isoforms expressed in B lymphocytes J Immunol 152 574 584 8283039
- Waldmann TA Strober W 1969 Metabolism of immunoglobulins Progress in Allergy 13 1 110 4186070
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Wilcox, C. R., Holder, B., & Jones, C. E. (2017) · Frontiers in Immunology · doi:10.3389/fimmu.2017.01294 · Cited by 200 · see citing works
“However, they differ from humans in many key features including levels of FcRn expression (132), immunological function (133), and placental anatomy (77).”
- Authors
- Mohanty, S., Kim, J., Ganesan, L. P., Phillips, G. S., Hua, K., Jarjoura, D., Hayton, W. L., Robinson, J. M., & Anderson, C. L.
- Affiliations
- The Ohio State University (US)
- Corresponding author
- Anderson, Clark L.
- Venue
- Journal of Reproductive Immunology
- Publisher
- Elsevier BV
- Published
- 2010-03
- Volume
- 84
- Issue
- 2
- Pages
- 133-144
- ISSN
- 0165-0378 (ISSN-L)
- PMC
- PMC3050502
- OpenAlex
- W2015985856
- Cited by
- 29
- References
- 1
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 1 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 4 · 2015: 5 · 2014: 2 · 2013: 5 · 2012: 1
- FWCI
- 0.58
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Reproductive Immunology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIH R01 (HD38764, CA88053, AI57530)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Keywords
- IgG transport, FcγRIIB, yolk sac, neonatal Fc receptor, placental endothelium, pericytes, syncytiotrophoblast, placental villi, mouse embryo
- MeSH terms
- Animals, Female, Humans, Immunoglobulin G, Male, Pregnancy, Species Specificity, Transferrin, Yolk Sac, Receptors, IgG, Protein Isoforms, Placental Circulation, Protein Transport, Mice
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