The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Helical reconstruction in RELION
Open accessAbstract
Abstract We describe a new implementation for the reconstruction of helical assemblies in the empirical Bayesian framework of RELION. Our approach calculates optimal linear filters for the 3D reconstruction by embedding helical symmetry operators in Fourier-space, and deals with deviations from perfect helical symmetry through Gaussian-shaped priors on the orientations of individual segments. By incorporating our approach into the standard pipeline for single-particle analysis in RELION, our implementation aims to be easily accessible for non-experienced users. Although our implementation does not solve the problem that grossly incorrect structures can be obtained when the wrong helical symmetry is imposed, we show for four different test cases that it is capable of reconstructing structures to near-atomic resolution. Abbreviations CARD Caspase Activation and Recruitment Domain EM Electron Microscopy FSC Fourier Shell Correlation IHRSR Iterative Helical Real-Space Reconstruction MAVS Mitochondrial Antiviral Signaling Protein TMV Tobacco Mosaic Virus
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Maximum Likelihood from Incomplete Data Via the EM Algorithm
Dempster, A. P., Laird, N. M., & Rubin, D. B. (1977) · Journal of the Royal Statistical Society Series B: Statistical Methodology · doi:10.1111/j.2517-6161.1977.tb01600.x · Cited by 50,444
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Self-citationStructure-based classification of tauopathies
Shi, Y., Zhang, W., Yang, Y., Murzin, A. G., Falcon, B., Kotecha, A., van Beers, M., Tarutani, A., Kametani, F., Garringer, H. J., et al. (2021) · Nature · doi:10.1038/s41586-021-03911-7 · Cited by 878
Self-citationCryo-EM structure of islet amyloid polypeptide fibrils reveals similarities with amyloid-β fibrils
Röder, C., Kupreichyk, T., Gremer, L., Schäfer, L. U., Pothula, K. R., Ravelli, R. B. G., Willbold, D., Hoyer, W., & Schröder, G. F. (2020) · Nature Structural & Molecular Biology · doi:10.1038/s41594-020-0442-4 · Cited by 180
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GSK3β phosphorylation catalyzes the aggregation of tau into Alzheimer’s disease-like filaments
Chakraborty, P., Ibáñez de Opakua, A., Purslow, J. A., Fromm, S. A., Chatterjee, D., Zachrdla, M., Zhuang, S., Puri, S., Wolozin, B., & Zweckstetter, M. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2414176121 · Cited by 96
Metadata
- Authors
- He, S., & Scheres, S. H. W.
- Venue
- Journal of Structural Biology
- Publisher
- Elsevier BV
- Published
- 2017-06
- Volume
- 198
- Issue
- 3
- Pages
- 163-176
- ISSN
- 1047-8477 (ISSN-L)
- OpenAlex
- W2951384153
- Cited by
- 561
- References
- 1
- FWCI
- 18.39
- Type
- journal article
- Language
- en
- Open access
- hybrid
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