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Cited by 26Renal Diseases and GlomerulopathiesChronic Kidney Disease and DiabetesNephrotoxicity and Medicinal Plants

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Effects of asiaticoside on levels of podocyte cytoskeletal proteins and renal slit diaphragm proteins in adriamycin-induced rat nephropathy

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Wang, Zhu, Liu, Juntian, Sun, Wansen·2013·Life Sciences
DOI 10.1016/j.lfs.2013.07.010
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Abstract

Aims Centella asiatica has been used to treat kidney diseases in Chinese traditional medicine. Asiaticoside (an extraction of C. asiatica) exerts a variety of pharmacological effects including immunomodulatory and anti-inflammatory functions. However, the mechanism of asiaticoside in the treatment of renal diseases remains largely unknown. This study investigated the molecular mechanism of asiaticoside in treating adriamycin-induced nephropathy of rats. Main methods Sixty-two SD male rats were randomly divided into normal control group (n=12) and nephropathy group (n=50). Except for the normal control group, rats were injected with adriamycin (6mg/kg) via the tail vein to induce nephropathy. Adriamycin induced nephropathic rats were divided into untreated group, prednisone group (25mg/kg), and asiaticoside groups with various dosages (8, 16 and 32mg/kg). Samples of urine and serum, tissue of kidney were collected for analysis after treatments for four weeks. Morphological changes were evaluated under light microscope and electron microscope. Synaptopodin, desmin, nephrin and podocin mRNA and protein were determined by RT-PCR and Western blotting. Key findings Compared to the untreated nephropathy group, asiaticoside treatment mitigated histological damages, decreased 24-hour urine protein excretion and total cholesterol, increased serum albumin. Asiaticoside treatment increased the mRNA and protein levels of synaptopodin, nephrin and podocin in a dose-dependent manner. Furthe…

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Metadata

Authors
Wang, Z., Liu, J., & Sun, W.
Affiliations
  • Second Affiliated Hospital of Xi'an Jiaotong University (CN)
  • Xi'an Jiaotong University (CN)
Corresponding author
Liu, Juntian
ORCID
Liu, Juntian
Venue
Life Sciences
Publisher
Elsevier BV
Published
2013-09
Volume
93
Issue
8
Pages
352-358
ISSN
0024-3205 (ISSN-L)
DOI
10.1016/j.lfs.2013.07.010
OpenAlex
W2002253678
Cited by
26
References
0
Citations by year
2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 5 · 2020: 2 · 2019: 5 · 2018: 1 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 1
FWCI
1.51
Type
journal article
Language
en
Open access
Licence
Locations
  • Life Sciences (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Nephrology › Renal Diseases and Glomerulopathies
  • Health Sciences › Medicine › Nephrology › Chronic Kidney Disease and Diabetes
  • Health Sciences › Medicine › Nephrology › Nephrotoxicity and Medicinal Plants
Keywords
asiaticoside, Centella asiatica, adriamycin nephropathy, podocytes, cytoskeletal proteins, synaptopodin, nephrin, podocin, desmin, slit diaphragm proteins, proteinuria, renal protection, prednisone
MeSH terms
Animals, Antibiotics, Antineoplastic, Cytoskeletal Proteins, Dose-Response Relationship, Drug, Doxorubicin, Gene Expression Regulation, Kidney Diseases, Male, Medicine, Chinese Traditional, Membrane Proteins, Microscopy, RNA, Messenger, Triterpenes, Blotting, Western, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Centella, Podocytes, Rats

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