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Cited by 12Sarcoma Diagnosis and TreatmentAdvanced Proteomics Techniques and ApplicationsAcute Lymphoblastic Leukemia research

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Detection of early prognostic biomarkers for metastasis of Ewing's sarcoma in pediatric patients

Ezzeldin, Shahd, Osama, Aya, Anwar, Ali Mostafa, Mahgoub, Sebaey, Ahmed, Eman A., Farid, Nesma, Zamzam, Manal, El Ghoneimy, Ahmed, Magdeldin, Sameh·2023·Life Sciences
DOI 10.1016/j.lfs.2023.122237
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Abstract

Aims Ewing's Sarcoma is an extremely aggressive tumor in children. The disease is associated with highly metastatic rate, especially at the time of diagnosis, contributing to a lower survival rate and poor prognosis. The study aimed to identify predictive biomarkers for metastatic Ewing's sarcoma through in-depth analysis of the plasma proteome profile of pediatric Ewing's sarcoma patients. Main methods Plasma samples from Ewing's sarcoma patients and control individuals were profiled using both shotgun and dimethyl-labeled proteomics analysis. Subsequently, Ewing's sarcoma patients were further stratified according to their metastatic state and chemotherapy response. Western blot was used for validation. Univariate and multivariate analyses were performed to determine proteome metastasis predictors. Receiver operating characteristic (ROC) analysis was done to assess the diagnostic significance of the potential plasma Ewing's sarcoma biomarkers. Key findings Our results revealed a set of proteins significantly associated with the metastatic Ewing's sarcoma disease profile. These proteins include ceruloplasmin and several immunoglobulins. Additionally, our study disclosed significant differentially expressed proteins in pediatric Ewing's sarcoma, including CD5 antigen-like, clusterin, and dermcidin. Stable isotope dimethyl labeling and western blot further confirmed our results, strengthening the impact of such proteins in disease development. Furthermore, an unbiased ROC curve evaluated and confirmed the predictive power of these biomarker candidates. Significance This study presented potential empirical predictive circulating biomarkers for determining the disease status of pediatric Ewing's sarcoma, which is vital for early prediction.

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Metadata

Authors
Ezzeldin, S., Osama, A., Anwar, A. M., Mahgoub, S., Ahmed, E. A., Farid, N., Zamzam, M., El Ghoneimy, A., & Magdeldin, S.
Affiliations
  • Children Cancer Hospital (EG)
  • Suez Canal University (EG)
Corresponding author
Magdeldin, Sameh
ORCID
Venue
Life Sciences
Publisher
Elsevier BV
Published
2023-12
Volume
334
Pages
122237
ISSN
0024-3205 (ISSN-L)
DOI
10.1016/j.lfs.2023.122237
OpenAlex
W4388347112
Cited by
12
References
1
Citations by year
2026: 3 · 2025: 6 · 2024: 3
FWCI
2.22
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Life Sciences (publishedVersion)
  • PubMed (publishedVersion)
Funders
Egypt Cancer Network
Topics
  • Health Sciences › Medicine › Oncology › Sarcoma Diagnosis and Treatment
  • Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
  • Health Sciences › Medicine › Hematology › Acute Lymphoblastic Leukemia research
Keywords
Ewing sarcoma, childhood cancer, metastasis, prognostic factors, plasma proteome, circulating biomarkers, ceruloplasmin, immunoglobulins, clusterin, dermcidin, chemotherapy response, early detection
MeSH terms
Bone Neoplasms, Child, Humans, Prognosis, Sarcoma, Ewing, Proteome

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