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Cited by 19Microbial Inactivation MethodsEnzyme Production and CharacterizationEnzyme Catalysis and Immobilization

The DOI matches its Crossref record.

Comparative effects of high isostatic pressure and thermal processing on the inactivation of Rhizomucor miehei protease

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Leite Júnior, Bruno Ricardo de Castro, Tribst, Alline Artigiani Lima, Cristianini, Marcelo·2016·LWT
DOI 10.1016/j.lwt.2015.09.042
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Abstract

This study compared the inactivation of the proteolytic (PA) and milk-clotting (MCA) activities of Rhizomucor miehei protease by high isostatic pressure (HIP) up to 600 MPa/20 min/25 °C and by a thermal process (TP) up to 72.0°C/30 min. Pressures above 300 MPa caused a loss of enzyme activity, MCA being expressively more affected than PA. The inactivation of the protease showed a nonlinear behavior, converging to a two-component system model (stable and labile fractions). The stable fraction represents higher activity for PA and the labile for MCA. The D-values for stable PA (3.35 ± 0.42 min for HIP and 5.58 ± 0.48 min for TP) and labile MCA (0.34 ± 0.04 min for HIP and 0.47 ± 0.04 min for TP) were significantly lower for the HIP processed enzyme, highlighting the effectiveness of the process in inactivating this enzyme. Therefore, the results highlight HIP at 600 MPa as an optional process to inactivate R. miehei protease (without the use of heat), being more effective than the thermal process.

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Research works printed but not linked: 14

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Metadata

Authors
Leite Júnior, B. R. D. C., Tribst, A. A. L., & Cristianini, M.
Affiliations
Universidade Estadual de Campinas (UNICAMP) (BR)
ORCID
Tribst, Alline Artigiani Lima
Venue
LWT
Publisher
Elsevier BV
Published
2016-01
Volume
65
Pages
1050-1053
ISSN
0023-6438 (ISSN-L)
DOI
10.1016/j.lwt.2015.09.042
OpenAlex
W1864851264
Cited by
19
References
0
Citations by year
2025: 1 · 2023: 4 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 8
FWCI
2.77
Type
journal article
Language
en
Open access
Licence
Locations
  • LWT (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion)
  • Americanae (AECID Library) (submittedVersion, cc-by-nc-nd)
Funders
Fundação de Amparo à Pesquisa do Estado de São Paulo (2012/13509-6, 2014/17782-4)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
Keywords
thermal processing, enzyme inactivation, proteolytic activity, milk-clotting activity, D-value

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