Characterization of S-layer proteins of potential probiotic starter culture Lactobacillus brevis SF9B isolated from sauerkraut
Open accessAbstract
S-layers represent the simplest biological membranes developed during the evolution and are one of the most abundant biopolymers on Earth. Current fundamental and applied research aim to reveal the chemical structure, morphogenesis and function of S-layer proteins (Slps). This is the first paper that describes the Slps of certain Lactobacillus brevis strain isolated from sauerkraut. The whole genome sequence (WGS) analysis of the L. brevis SF9B strain uncovered three genes encoding the putative Slps, but merely one, identified as similar to the SlpB of L. brevis ATCC 14869, was expressed. Slp-expressing SF9B cells exhibited increased survival in simulated gastrointestinal (GI) conditions and during freeze-drying. Their survival in stress conditions was additionally enhanced by microencapsulation, especially when using alginate with gelatine as a matrix. Thus prepared cells were subjected to simulated GI conditions and their mortality was only 0.28 ± 0.45 log CFU/mL. Furthermore, a correlation between the high surface hydrophobicity and the remarkable aggregative capacity of SF9B strain was established. The results indicate a prominent role of Slps in adhesion to mucin, extracellular matrix (ECM) proteins, and particularly to Caco-2 cells, where the removal of Slps utterly abolished the adhesiveness of SF9B cells for 7.78 ± 0.25 log CFU/mL.
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Besides surface layer proteins, other surface structures such as lipoteichoic acids and pili, passive forces, electrostatic interactions, and hydrophobicity affect probiotics' adherence to the host.
“The literature acknowledges that besides surface layer proteins, other surface structures such as lipoteichoic acids and pili, passive forces, electrostatic interactions, and hydrophobicity affects the probiotics' adherence to the host [28,50].”
From The Biological Role of the S-Layer Produced by Lactobacillus helveticus 34.9 in Cell Protection and Its Probiotic Properties (2024) · cites this paper as [28]
The discussion (page not available in our copy) says that lipoteichoic acid can mediate adhesion in an S-layer-deficient strain and that cell-surface hydrophobicity may facilitate adherence. It does not establish the claimed roles of pili, passive forces, or electrostatic interactions. (LLM, conf=0.88)
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References 53 · Cited by 35
Research works printed but not linked: 1
- Perkins, D. N., Pappin, D. J., Creasy, D. M., & Cottrell, J. S. (1999). Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis, 20(18), 3551-3567.
- Authors
- Banić, M., Uroić, K., Leboš Pavunc, A., Novak, J., Zorić, K., Durgo, K., Petković, H., Jamnik, P., Kazazić, S., Kazazić, S., Radović, S., Scalabrin, S., Hynӧnen, U., Šušković, J., & Kos, B.
- Affiliations
- University of Zagreb (HR)
- University of Ljubljana (SI)
- Ruđer Bošković Institute (HR)
- Istituto di Genomica Applicata (IT)
- Corresponding author
- Kos, Blaženka
- ORCID
- Venue
- LWT
- Publisher
- Elsevier BV
- Published
- 2018-07
- Volume
- 93
- Pages
- 257-267
- ISSN
- 0023-6438 (ISSN-L)
- OpenAlex
- W2791901385
- Cited by
- 35
- References
- 53
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 8 · 2023: 4 · 2022: 6 · 2021: 6 · 2020: 4 · 2019: 1
- FWCI
- 2.59
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Locations
- LWT (publishedVersion)
- Työväentutkimus Vuosikirja (submittedVersion)
- Funders
- Croatian Science Foundation (IP-2014-09-7009)
- University of Zagreb (2439-2016)
- Slovenian Research Agency (P4-0116)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Agricultural and Biological Sciences › Food Science › Microencapsulation and Drying Processes
- Keywords
- S-layer proteins, Lactobacillus brevis, probiotics, sauerkraut, probiotic starter cultures, gastrointestinal survival, freeze-drying, microencapsulation, surface hydrophobicity, cell aggregation, mucin adhesion, Caco-2 cells, whole genome sequencing
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