The DOI matches its Crossref record; 53 of the 54 references it cites were found in a registry or an index.
Insulin and insulin-like growth factor-1 can modulate the phosphoinositide-3-kinase/Akt/FoxO1 pathway in SZ95 sebocytes in vitro
Abstract
A recent hypothesis suggests that a high glycaemic load diet-associated increase of insulin-like growth factor-1 (IGF-1) and insulin may promote acne by reducing nuclear localization of the forkhead box-O1 (FoxO1) transcription factor via activation of the phosphoinositide-3-kinase (PI3K)/Akt pathway. Using SZ95 sebocytes as a model, we investigated the effect of the most important insulinotropic western dietary factors, IGF-1 and insulin on acne. SZ95 sebocytes were stimulated with different concentrations of IGF-1 and insulin (0.001, 0.01, 0.1 and 1 μM) for 15 to 120 min ± PI3K inhibitor LY294002 (50 μM). Cytoplasmic and nuclear protein expression of p-Akt and p-FoxO1 as well as FoxO transcriptional activity was analysed. In addition, the proliferation and differentiation of sebocytes and their TLR2/4 expression were determined. We found that high concentrations of IGF-1 and insulin differentially stimulate the PI3K/Akt/FoxO1 pathway by an early up-regulation of cytoplasmic p-Akt and delayed up-regulation of p-FoxO1 resulting in FoxO1 shift to the cytoplasm and the reduction of FoxO transcriptional activity, physiological serum concentration had no effect. IGF-1 at concentrations of 0.1 and 1 μM significantly reduced proliferation but increased differentiation of sebocytes to a greater extent than insulin (0.1 and 1 μM), but up-regulated TLR2/4 expression to comparable extent. These data provide the first in vitro evidence that FoxO1 principally might be involved in the regulation of growth-factor-stimulatory effects on sebaceous lipogenesis and inflammation in the pathological condition of acne. However, the in vivo significance under physiological conditions remains to be elucidated.
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“The presence of the IGF-1 receptor in sebocytes has been described and was shown to activate the PI3K/ Akt/FoxO1 pathway”
From Sebaceous-immunobiology is orchestrated by sebum lipids (2017) · cites this paper as [84]
In the citing paper
“thus providing evidence that FoxO1 may be a key factor in the regulation of growth-factor stimulatory effects on sebaceous lipogenesis and inflammation in response to elevated IGF-1 and insulin levels.84”
From Sebaceous-immunobiology is orchestrated by sebum lipids (2017) · cites this paper as [84]
Citations
Cites
Dynamic FoxO transcription factors
Huang, H., & Tindall, D. J. (2007) · Journal of Cell Science · doi:10.1242/jcs.001222 · Cited by 1,124
Thiboutot, D. M., Gollnick, H. P. M., Bettoli, V., Dréno, B., Kang, S., Leyden, J. J., Shalita, A. R., Lozada, V. T., Berson, D. S., Finlay, A. Y., et al. (2009) · Journal of the American Academy of Dermatology · doi:10.1016/j.jaad.2009.01.019 · Cited by 863
Self-citationGollnick, H. P. M., Cunliffe, W. J., Berson, D. S., Dréno, B., Finlay, A. Y., Leyden, J. J., Shalita, A. R., & Thiboutot, D. M. (2003) · Journal of the American Academy of Dermatology · doi:10.1067/mjd.2003.618 · Cited by 818
Self-citationToll-like receptor 2–mediated NF-κB activation requires a Rac1-dependent pathway
Arbibe, L., Mira, J. P., Teusch, N. E., Kline, L., Guha, M., Mackman, N., Godowski, P. J., Ulevitch, R. J., & Knaus, U. G. (2000) · Nature Immunology · doi:10.1038/82797 · Cited by 664
New developments in our understanding of acne pathogenesis and treatment
Kurokawa, I., Danby, F. W., Ju, Q., Wang, X., Xiang, L. F., Xia, L., Chen, W., Nagy, I., Picardo, M., Suh, D. H., et al. (2009) · Experimental Dermatology · doi:10.1111/j.1600-0625.2009.00890.x · Cited by 584
Self-citationFoxO1 Regulates Multiple Metabolic Pathways in the Liver
Zhang, W., Patil, S., Chauhan, B., Guo, S., Powell, D. R., Le, J., Klotsas, A., Matika, R. W., Xiao, X., Franks, R. R., et al. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m600272200 · Cited by 522
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Cordain, L., Lindeberg, S., Hurtado, M., Hill, K., Eaton, S. B., & Brand-Miller, J. (2002) · Archives of Dermatology · doi:10.1001/archderm.138.12.1584 · Cited by 500
Role of Hormones in Pilosebaceous Unit Development
Deplewski, D., & Rosenfield, R. L. (2000) · Endocrine Reviews · doi:10.1210/edrv.21.4.0404 · Cited by 443
Activation of Toll-Like Receptor 2 in Acne Triggers Inflammatory Cytokine Responses1
Kim, J. J., Ochoa, M., Krutzik, S., Takeuchi, O., Uematsu, S., Legaspi, A., Brightbill, H., Holland, D., Cunliffe, W., Akira, S., et al. (2002) · Journal of Immunology · doi:10.4049/jimmunol.169.3.1535 · Cited by 429
Establishment and Characterization of an Immortalized Human Sebaceous Gland Cell Line (SZ95)1
Zouboulis, C. C., Seltmann, H., Orfanos, C. E., & Neitzel, H. (1999) · Journal of Investigative Dermatology · doi:10.1046/j.1523-1747.1999.00771.x · Cited by 363
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1 of the 54 references couldn’t be matched to a known paper.
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- Smith, 2008, IGF-1 induces SREBP-1 expression and lipogenesis in SEB-1 sebocytes via activation of the phosphoinositide 3-kinase/Akt pathway, J. Invest. Dermatol., 128, 1286, 10.1038/sj.jid.5701155 · DOI
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ALA-PDT in the Treatment of Diseases of Hair Follicles and Sebaceous Glands
Liu, X., Zhang, L., Cao, Y., Cao, Z. W., Yang, J., Wang, B., & Wang, X. (2025) · Springer eBooks · doi:10.1007/978-981-96-8915-6_9
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Fujita, S., Inoue, S., Zouboulis, C. C., Fukuda, T., Hashizume, T., & Itoh, T. (2025) · Molecules · doi:10.3390/molecules30163360
Self-citationPathogenetically directed action of dermatocosmetics in acne
Kruglova, L. S., & Gryazeva, N. V. (2025) · Medical alphabet · doi:10.33667/2078-5631-2025-23-40-42
The Impact of Diet Composition on the Reduction of Acne Vulgaris: A Crossover Study
Daszkiewicz, M., Różańska, D., & Regulska−Ilow, B. (2026) · Cosmetics · doi:10.3390/cosmetics13030115
Petriy, M. A., Горбач, А. В., & Круглова, Л. С. (2026) · Restorative Dermatology and Venereology and Cosmetology · doi:10.46393/3034722x_2026_1_40-48
Magharehabed, Y., Ibrahim, Z., Shiekhtholth, H., & Yusuf, N. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27167123
Molecular Role of Carbohydrates in Acne and Seborrheic Dermatitis
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Metadata
- Authors
- Mirdamadi, Y., Thielitz, A., Wiede, A., Goihl, A., Papakonstantinou, E., Hartig, R., Zouboulis, C. C., Reinhold, D., Simeoni, L., Bommhardt, U., Quist, S., & Gollnick, H.
- Affiliations
- Otto-von-Guericke-Universität Magdeburg (DE)
- Städtisches Klinikum Dessau (DE)
- Corresponding author
- Gollnick, Harald
- Venue
- Molecular and Cellular Endocrinology
- Publisher
- Elsevier BV
- Published
- 2015-11
- Volume
- 415
- Pages
- 32-44
- ISSN
- 0303-7207 (ISSN-L)
- OpenAlex
- W1080640958
- Cited by
- 109
- References
- 54
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 7 · 2023: 5 · 2022: 10 · 2021: 14 · 2020: 14 · 2019: 6 · 2018: 13 · 2017: 17 · 2016: 9 · 2015: 1
- FWCI
- 14.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular and Cellular Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Department Dermatology & Venereology Otto-von-Guericke University Magdeburg/Germany
- Topics
- Health Sciences › Medicine › Dermatology › Acne and Rosacea Treatments and Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › FOXO transcription factor regulation
- Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
- Keywords
- insulin, insulin-like growth factor I, FOXO1, PI3K/Akt signaling, sebocytes, acne, lipogenesis, TLR2/4, LY294002
- MeSH terms
- Forkhead Box Protein O1, Acne Vulgaris, Cell Differentiation, Cell Line, Gene Expression Regulation, Humans, Insulin, Insulin-Like Growth Factor I, Models, Biological, Signal Transduction, Phosphatidylinositol 3-Kinases, Cell Proliferation, Lipogenesis, Proto-Oncogene Proteins c-akt, Toll-Like Receptor 2, Toll-Like Receptor 4, Forkhead Transcription Factors, In Vitro Techniques
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