Accumulation of gamma/delta T cells in the lungs and their roles in neutrophil-mediated host defense against pneumococcal infection
Open accessAbstract
The present study was designed to elucidate the role of Vgamma4(+) gammadelta T cells, a major subset of pulmonary gammadelta T cells, in host defense against infection with Streptococcus pneumoniae. The proportion and number of whole gammadelta T cells, identified as CD3(+) and TCR-delta(+) cells, and Vgamma4(+) gammadelta T cells, identified as CD3(+) and TCR-Vgamma4(+) cells, increased in the lungs at 3, 6 and 12h post-infection. Survival of infected mice and lung bacterial clearance were severely impaired in TCR-Vgamma4(-/-) mice compared with control wild-type (WT) mice. The impaired host protection in TCR-Vgamma4(-/-) mice correlated well with attenuated recruitment of neutrophils in lungs. MIP-2 and TNF-alpha synthesis in the infected tissues was significantly reduced in TCR-Vgamma4(-/-) mice compared with WT mice. Similar results were noted in the synthesis of TNF-alpha, but not clearly of MIP-2, by lung leukocytes stimulated with live bacteria. Our results demonstrate that Vgamma4(+) gammadelta T cells play an important role in the neutrophil-mediated host defense against S. pneumoniae infection by promoting the synthesis of TNF-alpha and possibly of MIP-2 in the lungs.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In humans, the subset of γδ T cells localized in human skin is Vδ1+ γδ T cells, which express oligoclonal clonal sequences distinct from circulating γδ T cells.
“In humans, the subset of γδ T cells localized in human skin is Vδ1+ γδ T cells, which express oligoclonal clonal sequences distinct from circulating γδ T cells (39).”
From Comparing Mouse and Human Tissue-Resident γδ T Cells (2022) · cites this paper as [39]
The Introduction and Discussion address γδ T-cell subsets in mouse infection and pulmonary inflammation, not human skin. Searches for human skin Vδ1+ cells, oligoclonal sequences, and differences from circulating γδ T cells found no passage addressing the claim. The cited paper’s copy therefore cannot verify it. (LLM, conf=0.76)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 49 · Cited by 100
Research works printed but not linked: 2
- D. Musher, M. Cohen, C. Baker, Immune responses to extracellular bac- teria, in: R.R. Rich, T.A. Fleisher, B.D. Schwartz, W.T. Shearer, W. Strober (Eds.), Clinical immunology: principles and practice, Mosby- Year Book, Inc, St. Louis, MO, 1996, pp. 479e502.
- M. Lahn, A. Kanehiro, K. Takeda, J. Terry, Y.S. Hahn, M.K. Aydintug, A. Konowal, K. Ikuta, R.L. O'Brien, E.W. Gelfand, W.K. Born, MHC class I-dependent Vgamma4þ pulmonary T cells regulate alpha beta T cell-independent airway responsiveness, Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 8850e8855.
- Authors
- Nakasone, C., Yamamoto, N., Nakamatsu, M., Kinjo, T., Miyagi, K., Uezu, K., Nakamura, K., Higa, F., Ishikawa, H., O'Brien, R. L., Ikuta, K., Kaku, M., Fujita, J., & Kawakami, K.
- Affiliations
- University of the Ryukyus (JP)
- Tohoku University (JP)
- Keio University (JP)
- Kyoto University (JP)
- Corresponding author
- Kawakami, Kazuyoshi
- ORCID
- Venue
- Microbes and Infection
- Publisher
- Elsevier BV
- Published
- 2007-03
- Volume
- 9
- Issue
- 3
- Pages
- 251-258
- ISSN
- 1286-4579 (ISSN-L)
- OpenAlex
- W1999905308
- Cited by
- 100
- References
- 49
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 6 · 2023: 9 · 2022: 5 · 2021: 5 · 2020: 4 · 2019: 5 · 2018: 4 · 2017: 5 · 2016: 4 · 2015: 7 · 2014: 8 · 2013: 6 · 2012: 4
- FWCI
- 1.25
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 6150 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Microbes and Infection (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- γδ T cells, Vγ4 T cells, Streptococcus pneumoniae, pneumococcal infection, neutrophil recruitment, host defense, TNF-α, CXCL2, pulmonary immune response
- MeSH terms
- Animals, Humans, Lung, Mice, Inbred C57BL, Neutrophils, Pneumonia, Pneumococcal, Streptococcus pneumoniae, T-Lymphocytes, Tumor Necrosis Factor-alpha, Virulence, Receptors, Antigen, T-Cell, gamma-delta, Chemokines, Neutrophil Infiltration, Mice, Chemokine CXCL2
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
