The role of HPV gene expression and selected cellular MiRNAs in lung cancer development
Abstract
Background The high mortality rate of lung cancer can be justified that strong need to explore new aspect of tumor biology. Human papillomavirus (HPV) has been detected as risk factor for the development of lung cancer. The aim of this study was to determine the role of HPV and cellular/miRNAs genes expression in the epithelial-mesenchymal transition (EMT) and development of lung cancer. Methods In this case-control study, 109 lung cancer tissue and 52 controls were included. We analyzed the presence of HPV infection, its genotypes (in positive samples) and the expression of viral genes (E2, E6 and E7). Also, We examined the expression of celluar factors including (a) p53 and retinoblastoma (Rb) (as anti-carcinogenic genes), (b) EMT related genes, (c) selected miRNAs. Results Our results reported 51.4% and 23.1% of HPV genome in tumor tissues and control tissues samples, respectively. There was a significant association between the HPV positive status and lung cancer (OR = 3.26, 95% C.I = 1.47-7.02, P = 0.001). HPV type 16 was the most prevalent genotype in tissues. The expression of p53, RB, TIMP1, CCNG-1, E-cad and PTPN13 were decreased while MMP-2 and N-cad were increased in HPV-positive tumor/control tissues compared to HPV-negative tissues. Also, among miRNAs, let-7, miR-23, miR-34, miR-125, miR-146 were downregulated and miR-20, miR-424 were upregulated in HPV-positve tissues compared to HPV-negative tissues. Conclusion This study demonstrated that HPV infection and int…
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In a clinical study involving 109 lung cancer tissue and 52 normal tissue samples, expression of miR-23a is significantly reduced in HPV-positive lung cancer tissue.
“A clinical study involving 109 cases of lung cancer tissue and 52 cases of normal tissue revealed that the expression of miR-23a is significantly reduced in HPV-positive lung cancer tissue.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [92]
This phenomenon is closely associated with the epithelial-mesenchymal transition process in lung cancer.
“This phenomenon is closely associated with the epithelial-mesenchymal transition (EMT) process in lung cancer”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [92]
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Recent advances of miR-23 in human diseases and growth development
Qian, X., Jiang, Y., Yang, Y., Zhang, Y., Xu, N., Xu, B., Pei, K., Yu, Z., & Wu, W. (2025) · Non-coding RNA Research · doi:10.1016/j.ncrna.2024.12.010 · Cited by 5 · see citing works
- Authors
- Hussen, B. M., Ahmadi, G., Marzban, H., Fard Azar, M. E., Sorayyayi, S., Karampour, R., Nahand, J. S., Hidayat, H. J., & Moghoofei, M.
- Affiliations
- Hawler Medical University (IQ)
- University of Maragheh (IR)
- Razi Vaccine and Serum Research Institute (IR)
- Ardabil University of Medical Sciences (IR)
- Razi University (IR)
- Iran University of Medical Sciences (IR)
- Salahaddin University-Erbil (IQ)
- Kermanshah University of Medical Sciences (IR)
- Corresponding author
- Moghoofei, Mohsen
- ORCID
- Venue
- Microbial Pathogenesis
- Publisher
- Elsevier BV
- Published
- 2021-01
- Volume
- 150
- Pages
- 104692
- ISSN
- 0882-4010 (ISSN-L)
- OpenAlex
- W3111775328
- Cited by
- 62
- References
- 0
- Citations by year
- 2026: 3 · 2025: 11 · 2024: 23 · 2023: 10 · 2022: 11 · 2021: 4
- FWCI
- 4.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Microbial Pathogenesis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Otorhinolaryngology › Head and Neck Cancer Studies
- Keywords
- human papillomavirus, lung cancer, epithelial-mesenchymal transition, microRNA, HPV16, p53, retinoblastoma protein, E-cadherin, N-cadherin, MMP-2, TIMP-1, let-7 microRNA, miR-34, miR-23, miR-146, miR-20, miR-424, viral oncogenes
- MeSH terms
- Humans, Lung Neoplasms, Oncogene Proteins, Viral, Gene Expression, Case-Control Studies, Papillomaviridae, Papillomavirus Infections, MicroRNAs, Alphapapillomavirus
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