Brazilian red propolis: Chemical composition and antibacterial activity determined using bioguided fractionation
Open accessAbstract
The indiscriminate use of antibiotics is causing an increase in bacterial resistance, complicating therapeutic planning. In this context, natural products have emerged as major providers of bioactive compounds. This work performs a bioguided study of Brazilian red propolis to identify compounds with antibacterial potential and to evaluate their cytotoxicity against non-tumour cells. Using bioguided fractionation performed with the hydroalcoholic extract of red propolis from Alagoas, it was possible to obtain subfractions with remarkable bacteriostatic activity compared with the precursor fractions. The SC2 subfraction was highlighted and showed the best results with minimal inhibitory concentrations (MICs) of 56.75, 28.37, 454.00, and 227.00 μg mL-1 against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa, respectively. However, this study also revealed a cytotoxic effect against the non-tumour Vero cell line. Furthermore, through chemical analyses using high resolution mass spectrometry, high performance liquid chromatography with UV detection, and gas chromatography coupled to mass spectrometry, we verified the presence of important marker compounds in the fractions and extracts, including formononetin (m/z 267.0663), biochanin A (m/z 283.0601), and liquiritigenin (m/z 255.0655). The results obtained in this study suggest an important antibacterial potential of red propolis subfractions. In this context, the bioguided fractionation has been a useful process, due to its ability to isolate and concentrate active compounds in a logical and rational way.
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Gram-negative bacteria have a more chemically complex cell wall with negatively charged lipopolysaccharides that act as a barrier, providing greater resistance.
“Gram-negative bacteria have a more chemically complex wall, with the presence of the negatively charged lipopolysaccharides, which act as a barrier, providing greater resistance [45].”
From Anti-Inflammatory and Histological Analysis of Skin Wound Healing through Topical Application of Mexican Propolis (2023) · cites this paper as [45]
The Discussion (page unavailable) says Gram-negative bacteria have a more chemically complex cell wall that provides greater resistance. It does not mention negatively charged lipopolysaccharides or identify them as the barrier responsible. (LLM, conf=0.88)
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Research works printed but not linked: 2
- Auerbach, T., Mermershtain, I., Davidovich, C., et al., 2010. The structure of ribosome- lankacidin complex reveals ribosomal sites for synergistic antibiotics. Proc. Natl. Acad. Sci. U. S. A. 107, 1983-1988.
- Ghisalberti, E.L., 1979. Própolis: a review. Bee World 60, 59-84.
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Balderas-Cordero, D., Canales-Alvarez, O., Sánchez-Sánchez, R., Cabrera-Wrooman, A., Canales-Martinez, M. M., & Rodriguez-Monroy, M. A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241411831 · Cited by 49 · see citing works
- Authors
- Rufatto, L. C., Luchtenberg, P., Garcia, C., Thomassigny, C., Bouttier, S., Henriques, J. A. P., Roesch-Ely, M., Dumas, F., & Moura, S.
- Affiliations
- Universidade de Caxias do Sul (BR)
- Université de Versailles Saint-Quentin-en-Yvelines (FR)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Dumas, Françoise
- Moura, Sidnei
- Venue
- Microbiological Research
- Publisher
- Elsevier BV
- Published
- 2018-09
- Volume
- 214
- Pages
- 74-82
- ISSN
- 0944-5013 (ISSN-L)
- OpenAlex
- W2802667260
- Cited by
- 109
- References
- 0
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 16 · 2023: 15 · 2022: 20 · 2021: 23 · 2020: 12 · 2019: 8 · 2018: 2
- FWCI
- 9.92
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Microbiological Research (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Ministère de l'Enseignement supérieur, de la Recherche et de l'Innovation
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul
- Centre National de la Recherche Scientifique
- Topics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Bee Products Chemical Analysis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry Medicinal Plant Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- Brazilian red propolis, bioguided fractionation, antibacterial activity, chemical composition, formononetin, biochanin A, liquiritigenin, flavonoids, cytotoxicity, Vero cells, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, antibiotic resistance, natural products
- MeSH terms
- Animals, Anti-Bacterial Agents, Bacteria, Biological Products, Brazil, Cell Survival, Chlorocebus aethiops, Chromatography, High Pressure Liquid, Epithelial Cells, Chemical Fractionation, Microbial Sensitivity Tests, Propolis, Mass Spectrometry, Vero Cells
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