’Ropy’ Phenotype, Exopolysaccharides and Metabolism: Study on Food Isolated Potential Probiotics LAB
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Lactic acid bacteria are fully recognized for their industrial applications among which the production and release of exopolysaccharides. In the present investigation, we screened fifteen Lactobacilli in order to find ropy strains, quantify exopolysaccharides and detect proteins specifically associated with the ropy-exopolysaccharide production. The highest ropy-exopolysaccharide producer (L. helveticus 6E8), was grown in stimulating and basal condition (10% and 2% lactose) and subjected to comparative proteomic analysis. The levels of 4 proteins were found significantly increased in the membrane fraction under stimulating conditions: a specific exopolysaccharide biosynthetic protein, a stress-induced protein, a protein involved in secretion and an ATP-synthase subunit. Conversely, several enzymes involved in anabolism and protein synthesis were decreased. These results suggest a general shift from growth to exopolysaccharide-mediated protection from the hyperosmotic environment. Due to the great interest in exopolysaccharides with novel features, the identification of these proteins could have implications for future improvements of industrial strains.
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The composition of the culture medium, including added nutrients as growth enhancers, is one of the most important factors affecting EPS production.
“The composition of the culture medium, including added nutrients as growth enhancers, is one of the most important factors affecting EPS production [55].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [55]
The Introduction says culture conditions can be manipulated to increase EPS production and gives the carbon-to-nitrogen ratio as an example. Section 3.2 discusses increased EPS biosynthesis in high-sugar media. The copy does not characterize added nutrients as growth enhancers or establish medium composition as one of the most important factors. (LLM, conf=0.82)
Experimental — accuracy not yet measuredMRS media supplemented with sucrose, fructose or maltose is generally used to screen HoPS production.
“For instance, de Man Rogosa and Sharpe (MRS) media supplemented with sucrose, fructose or maltose is generally used to screen HoPS production [37,55,56].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [55]
Sections 2.2 and 3.1 describe screening for the ropy phenotype using sugar-supplemented media, including sucrose and fructose. But the medium was semi-defined medium (SDM), not MRS; the tested sugars did not include maltose, and the screen was not described as specific to HoPS production. Page numbers are unavailable in our copy. (LLM, conf=0.85)
Experimental — accuracy not yet measuredThe presence of different carbohydrates as a carbon source can significantly affect the formation of ropy filaments, leading to misinterpretation of ropy phenotypes.
“Furthermore, the presence of different carbohydrates as a carbon source can significantly affect the formation of ropy filaments, leading to misinterpretation of ropy phenotypes [55].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [55]
Section 3.2 reports that lactose was better at inducing the ropy phenotype and cites another strain whose ropyness decreased with fructose as its sole sugar source. But in the strains studied here, RP-positive strains remained positive across the tested sugars; the paper does not show that carbohydrate choice caused a misinterpretation of their ropy phenotypes. Section 3.1 instead identifies scarce biomass as a difficulty in detecting filaments. (LLM, conf=0.84)
Experimental — accuracy not yet measuredThe phenol-sulfuric acid method is the most widely used method of calculating EPS yield.
“A colorimetric procedure for the determination of total carbohydrate (expressed as glucose equivalents per unit of weight) by phenol-sulfuric acid method is the most widely used method of calculating EPS yield [27,33,34,42,43,44,49,55,99,102].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [55]
Section 2.3.4 reports using the phenol-sulfuric-acid method with glucose as the standard to determine EPS content. The paper does not say it is the most widely used method for calculating EPS yield. (LLM, conf=0.84)
Experimental — accuracy not yet measuredEthanol is commonly used for precipitation of EPS, although isopropanol, acetone or a mixture of acetone and ethanol have also been used.
“For the following precipitation of EPS, ethanol is commonly used [27,42,43,50,55,58,99], although isopropanol [100], acetone [33] or a mixture of acetone and ethanol [101] have been also used.”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [55]
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References 65 · Cited by 53
- Authors
- Cirrincione, S., Breuer, Y., Mangiapane, E., Mazzoli, R., & Pessione, E.
- Affiliations
- University of Turin (IT)
- Hebrew University of Jerusalem (IL)
- Corresponding author
- Pessione, Enrica
- Venue
- Microbiological Research
- Publisher
- Elsevier BV
- Published
- 2018-09
- Volume
- 214
- Pages
- 137-145
- ISSN
- 0944-5013 (ISSN-L)
- OpenAlex
- W2874216812
- Cited by
- 53
- References
- 65
- Citations by year
- 2026: 7 · 2025: 11 · 2024: 5 · 2023: 5 · 2022: 12 · 2021: 8 · 2020: 5
- FWCI
- 3.05
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Microbiological Research (publishedVersion)
- PubMed (publishedVersion)
- Institutional Research Information System University of Turin (University of Turin) (submittedVersion, other-oa)
- Funders
- Hebrew University of Jerusalem
- Università degli Studi di Torino
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- exopolysaccharide production, lactic acid bacteria, Lactobacillus helveticus, probiotics, proteomics, hyperosmotic stress, stress proteins, ATP synthase, membrane proteins, lactose concentration, industrial strain improvement
- MeSH terms
- Bacterial Proteins, Bacteriological Techniques, Culture Media, Food Microbiology, Lactobacillus, Lactose, Phenotype, Polysaccharides, Bacterial, Probiotics, Proteome
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