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Cited by 42Flavonoids in Medical ResearchCellular Mechanics and InteractionsBioactive Compounds and Antitumor Agents

Morphological and mechanical changes induced by quercetin in human T24 bladder cancer cells

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Adami, Bruno Silveira, Diz, Fernando Mendonça, Oliveira Gonçalves, Gustavo Petry, Reghelin, Camille Kirinus, Scherer, Matheus, Dutra, Artur Pereira, Papaléo, Ricardo Meurer, de Oliveira, Jarbas Rodrigues, Morrone, Fernanda Bueno, Wieck, Andrea, Xavier, Léder Leal·2021·Micron
DOI 10.1016/j.micron.2021.103152
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Abstract

Quercetin is a flavonoid found in a great variety of foods such as vegetables and fruits. This compound has been shown to inhibit the proliferation of various types of cancer cells, as well as the growth of tumors in animal models. In the present study, we analyze morphological and mechanical changes produced by quercetin in T24 bladder cancer cells. Decreased cell viability and cell number were observed following quercetin treatment at 40 μM and 60 μM, respectively, as observed by the MTT assay and trypan blue exclusion test, supporting the hypothesis of quercetin anticancer effect. These assays also allowed us to determine the 40, 60, and 80 μM quercetin concentrations for the following analyses, Lactate Dehydrogenase assay (LDH); Nuclear Morphometric Analysis (NMA); and atomic force microscopy (AFM). The LDH assay showed no cytotoxic effect of quercetin on T24 cancer cells. The AFM showed morphological changes following quercetin treatment, namely decreased cell body, cytoplasmic retraction, and membrane condensation. Following quercetin treatment, the NMA evidenced an increased percentage of nuclei characteristic to the apoptotic and senescence processes. Cells also presented biophysical alterations consistent with cell death by apoptosis, as increased roughness and aggregation of membrane proteins, in a dose-dependent manner. Cellular elasticity, obtained through force curves, showed increased stiffness after quercetin treatment. Data presented herein demonstrate, for the first time, in a quantitative and qualitative form, the morphological and mechanical alterations induced by quercetin on bladder cancer cells.

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  • In T24 bladder cancer cells, quercetin treatment led to a marked increase in the percentage of cell nuclei during cellular senescence.

    “For example, in T24 bladder cancer cells, nuclear morphology analysis (NMA) revealed that quercetin treatment led to a marked increase in the percentage of cell nuclei during cellular senescence.237”

    From Drug repurposing for cancer therapy (2024) · cites this paper as [237]

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Authors
Adami, B. S., Diz, F. M., Oliveira Gonçalves, G. P., Reghelin, C. K., Scherer, M., Dutra, A. P., Papaléo, R. M., de Oliveira, J. R., Morrone, F. B., Wieck, A., & Xavier, L. L.
Affiliations
Pontifícia Universidade Católica do Rio Grande do Sul (BR)
ORCID
Venue
Micron
Publisher
Elsevier BV
Published
2021-12
Volume
151
Pages
103152
ISSN
0968-4328 (ISSN-L)
DOI
10.1016/j.micron.2021.103152
OpenAlex
W3203777655
Cited by
42
References
70
Citations by year
2026: 5 · 2025: 5 · 2024: 9 · 2023: 15 · 2022: 8
FWCI
4.67
Type
journal article
Language
en
Open access
Licence
Locations
  • Micron (publishedVersion)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
  • PUCRS Repository (Pontifical Catholic University of Rio Grande do Sul) (submittedVersion, cc-by-nc-sa)
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ((Code 001))
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (Universal 2018, 409272/2018-3, 2018‐3)
Topics
  • Health Sciences › Medicine › Pharmacology (medical) › Flavonoids in Medical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
Keywords
quercetin, flavonoids, bladder cancer, T24 cells, apoptosis, cell stiffness, cell morphology, nuclear morphometry, membrane condensation, cell death, anticancer agents
MeSH terms
Animals, Urinary Bladder Neoplasms, Cell Survival, Humans, Quercetin, Apoptosis, Cell Line, Tumor

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