The DOI matches its Crossref record; 98 of the 98 references it cites were found in a registry or an index.
Methods for extracting ’omes from microbialites
Abstract
Microbialites are organo-sedimentary structures formed by complex microbial communities that interact with abiotic factors to form carbonate rich fabrics. Extraction of DNA or total RNA from microbialites can be difficult because of the high carbonate mineral concentration and exopolymeric substances. The methods employed until now include substances such as cetyltrimethylammonium bromide, sodium dodecyl sulfate, xanthogenate, lysozyme and proteinase K, as well as mechanical disruption. Additionally, several commercial kits have been used to improve DNA and total RNA extraction. This minireview presents different methods applied for DNA and RNA extraction from microbialites and discusses their advantages and disadvantages. Moreover, extraction of all 'omes (DNA, RNA, Protein, Lipids, polar metabolites) using multiomic extraction methods (MPlex), as well as the state of art for extraction of viruses from microbialites, are also discussed.
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“The DNA is still significantly contaminated with polyphenols, making it unsuitable for highly sensitive applications such as PacBio sequencing.7”
From Protocol for the transformation and engineering of edible algae Arthrospira platensis to generate heterologous protein-expressing strains (2023) · cites this paper as [7]
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Tan, R., Dong, H., Chen, Z., Jin, M., Yin, J., Li, H., Shi, D., Shao, Y., Wang, H., Chen, T., et al. (2021) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2021.654074 · Cited by 100
Recent Advances in Strategies for the Cloning of Natural Product Biosynthetic Gene Clusters
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Substratum‐Associated Microbiota
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Metadata
- Authors
- Gómez-Acata, E. S., Centeno, C. M., & Falcón, L. I.
- Affiliations
- Instituto de Ecología (MX)
- Venue
- Journal of Microbiological Methods
- Publisher
- Elsevier BV
- Published
- 2019-05
- Volume
- 160
- Pages
- 1-10
- ISSN
- 0167-7012 (ISSN-L)
- OpenAlex
- W2920809351
- Cited by
- 15
- References
- 98
- Citations by year
- 2026: 3 · 2025: 1 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 3
- FWCI
- 0.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Microbiological Methods (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Bacterial Ecology Laboratory
- UNAM (UCMEXUS CN116, UNAM PAPIIT 202016-3)
- Topics
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Physical Sciences › Environmental Science › Ecology › Environmental DNA in Biodiversity Studies
- Keywords
- microbialites, DNA extraction, RNA extraction, exopolymers, microbial communities, polar metabolites
- MeSH terms
- DNA, Bacterial, RNA, Bacterial, Viruses, Geologic Sediments, Metagenome, Microbiota
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