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Cited by 31Parasitic Diseases Research and TreatmentBiochemical and Molecular ResearchAdenosine and Purinergic Signaling

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A nucleotidase with unique catalytic properties is secreted by Trichinella spiralis

Gounaris, Kleoniki, Selkirk, Murray E., Sadeghi, Sheila J.·2004·Molecular and Biochemical Parasitology
DOI 10.1016/j.molbiopara.2004.04.008
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Abstract

We have isolated and expressed a cDNA from the parasitic nematode Trichinella spiralis encoding a novel secreted nucleotidase which catalyses the hydrolysis of nucleoside 5'-diphosphates and 5'-monophosphates, but not 5'-triphosphates. The full length cDNA encodes a protein of 550 amino acids with an N-terminal signal peptide, but lacking a C-terminal signature sequence for addition of a glycosyl phosphatidylinositol (GPI) anchor. Expression in Pichia pastoris resulted in the secretion of an active enzyme with the catalytic properties of both a Mg2+-dependent diphosphohydrolase/apyrase and a 5'-nucleotidase. The protein sequence is homologous to 5'-nucleotidases from a wide variety of organisms but contains no sequences specifically conserved in apyrases, suggesting that it is a representative of a new class of secreted nucleotidase. The enzyme was essentially monospecific for AMP among the nucleoside 5'-monophosphates and catalysed the hydrolysis of nucleoside 5'-diphosphates in the order of UDP >> ADP. The diphosphatase activity was dependent on the presence of magnesium ions and a reducing agent, while the 5'-nucleotidase activity was enhanced by these additions. Kinetic analyses indicated that the enzyme exhibits allosteric behaviour. Determination of the number of active sites suggested that catalysis of the two different reactions occurs at the same active site. The data are discussed in terms of regulation of host purinergic signalling during infection.

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Metadata

Authors
Gounaris, K., Selkirk, M. E., & Sadeghi, S. J.
Affiliations
  • Imperial College London (GB)
  • University of California, San Francisco (US)
Corresponding author
Gounaris, Kleoniki
ORCID
Venue
Molecular and Biochemical Parasitology
Publisher
Elsevier BV
Published
2004-08
Volume
136
Issue
2
Pages
257-264
ISSN
0166-6851 (ISSN-L)
DOI
10.1016/j.molbiopara.2004.04.008
OpenAlex
W2043601993
Cited by
31
References
0
Citations by year
2022: 1 · 2021: 1 · 2019: 1 · 2018: 2 · 2016: 3 · 2015: 2 · 2014: 3 · 2012: 3
FWCI
0.99
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Molecular and Biochemical Parasitology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Wellcome Trust
Topics
  • Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
Keywords
Trichinella spiralis, 5'-nucleotidase, apyrase, AMP hydrolysis, magnesium-dependent enzyme, allosteric regulation, single active site, Pichia pastoris expression, signal peptide
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Catalysis, Cloning, Molecular, Kinetics, Molecular Sequence Data, Pichia, Recombinant Proteins, 5'-Nucleotidase, Gene Expression, Trichinella spiralis, Genes, Helminth, Sequence Homology, Amino Acid, DNA, Complementary, DNA, Helminth

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