Selectively enriched mRNAs in rat synaptoneurosomes
Abstract
Differential display was used to identify synapse-enriched mRNAs. Of 15 mRNAs initially identified, all were found in multiple synaptoneurosome preparations; 58% were subsequently shown to be enriched in all the preparations by Northern blotting and semiquantitative RT-PCR. RNAs involved in signal transduction, vesicle trafficking, lipid modification and cell shape and remodeling were among these messages. Tip60a mRNA, recently found to associate with the fragile X mental retardation protein, was also identified. These data demonstrate the diversity of the local message pool at synapses.
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APP mRNA is present in synaptoneurosomes and coimmunoprecipitates with anti-FMRP but not control antisera in cortical lysates and synaptoneurosomes.
“Contrary to a previous report utilizing a different protocol for the preparation of SNs [26], APP mRNA is present in SNs, and reverse transcription (RT)–PCR revealed that the message was brought down with specific, but not control, antisera in cortical lysates as well as SNs (unpublished data).”
From FMRP Mediates mGluR5-Dependent Translation of Amyloid Precursor Protein (2007) · cites this paper as [26]
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Zalfa, F., Giorgi, M., Primerano, B., Moro, A., Di Penta, A., Reis, S., Oostra, B., & Bagni, C. (2003) · Cell · doi:10.1016/s0092-8674(03)00079-5 · Cited by 676 · see citing works
Weiler, I. J., Irwin, S. A., Klintsova, A. Y., Spencer, C. M., Brazelton, A. D., Miyashiro, K., Comery, T. A., Patel, B., Eberwine, J., & Greenough, W. T. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.10.5395 · Cited by 644 · see citing works
Localization and translation of mRNA in dentrites and axons
Job, C., & Eberwine, J. (2001) · Nature Reviews Neuroscience · doi:10.1038/35104069 · Cited by 202 · see citing works
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FMRP Mediates mGluR5-Dependent Translation of Amyloid Precursor Protein
Westmark, C. J., & Malter, J. S. (2007) · PLoS Biology · doi:10.1371/journal.pbio.0050052 · Cited by 292 · see citing works
“Contrary to a previous report utilizing a different protocol for the preparation of SNs [26], APP mRNA is present in SNs, and reverse transcription (RT)–PCR revealed that the message was brought down with specific, but not control, antisera in cortical lysates as well as SNs (unpublished data).”
ResultRetinoic acid-gated sequence-specific translational control by RARα
Poon, M. M., & Chen, L. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0807740105 · Cited by 138 · see citing works
Aberrant early-phase ERK inactivation impedes neuronal function in fragile X syndrome
Kim, S. H., Markham, J. A., Weiler, I. J., & Greenough, W. T. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0800257105 · Cited by 97 · see citing works
Self-citation
- Authors
- Sung, Y.-J., Weiler, I. J., Greenough, W. T., & Denman, R. B.
- Affiliations
- Columbia University (US)
- University of Illinois Urbana-Champaign (US)
- Corresponding author
- Denman, R.B
- Venue
- Molecular Brain Research
- Publisher
- Elsevier BV
- Published
- 2004-07
- Volume
- 126
- Issue
- 1
- Pages
- 81-87
- ISSN
- 0169-328X (ISSN-L)
- OpenAlex
- W2086910978
- Cited by
- 27
- References
- 3
- Citations by year
- 2019: 1 · 2017: 2 · 2016: 1 · 2014: 2
- FWCI
- 0.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- FRAXA Research Foundation
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- synaptoneurosomes, FMRP, signal transduction, vesicle trafficking, lipid modification, synapses
- MeSH terms
- Animals, Molecular Sequence Data, Neurons, RNA, Messenger, Synapses, Synaptosomes, Rats, Long-Evans, Gene Expression Profiling, Rats
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