Chromatin Disassembly Mediated by the Histone Chaperone Asf1 Is Essential for Transcriptional Activation of the Yeast PHO5 and PHO8 Genes
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Nucleosome loss from a promoter region has recently been described as a potential mechanism for transcriptional regulation. We investigated whether H3/H4 histone chaperones mediate the loss of nucleosomes from the promoter of the yeast PHO5 gene during transcriptional activation. We found that antisilencing function 1 (Asf1p) mediates nucleosome disassembly from the PHO5 promoter in vivo. We show that nucleosome disassembly also occurs at a second promoter, that of the PHO8 gene, during activation, and we demonstrate that this is also mediated by Asf1p. Furthermore, we show that nucleosome disassembly is essential for PHO5 and PHO8 activation. Contrary to the current dogma, we demonstrate that nucleosome disassembly is not required to enable binding of the Pho4p activator to its PHO5 UASp2 site in vivo. Finally, we show that nucleosomes are reassembled over the PHO5 promoter during repression. As such, nucleosome disassembly and reassembly are important mechanisms for transcriptional activation and repression, respectively.
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Histone chaperones and ATP-dependent chromatin-remodelling complexes are important for gene regulation because they regulate nucleosome dynamics at promoters, restricting or increasing promoter accessibility for recruitment of transcription factors and RNA polymerase II (Pol II).
“Histone chaperones and ATP-dependent chromatin-remodelling complexes are accordingly important for gene regulation given they regulate nucleosome dynamics at promoters, restricting or increasing promoter accessibility for recruitment of transcription factors and RNA polymerase II (Pol II)19–23.”
From SWI/SNF and the histone chaperone Rtt106 drive expression of the Pleiotropic Drug Resistance network genes (2022) · cites this paper as [22]
Histone chaperone Asf1 mediates nucleosome disassembly from the PHO5 promoter for its activation in yeast.
“It has been reported that histone chaperone Asf1 mediates nucleosome disassembly from the PHO5 promoter for its activation in yeast22,23.”
From SWI/SNF and the histone chaperone Rtt106 drive expression of the Pleiotropic Drug Resistance network genes (2022) · cites this paper as [22]
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References 44 · Cited by 294
- Authors
- Adkins, M. W., Howar, S. R., & Tyler, J. K.
- Affiliations
- University of Colorado Anschutz (US)
- Corresponding author
- Tyler, Jessica K
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2004-06
- Volume
- 14
- Issue
- 5
- Pages
- 657-666
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2067397907
- Cited by
- 294
- References
- 44
- Citations by year
- 2026: 1 · 2024: 4 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 4 · 2019: 6 · 2018: 8 · 2017: 6 · 2016: 2 · 2015: 8 · 2014: 11 · 2013: 8 · 2012: 11
- FWCI
- 14.60
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- March of Dimes Foundation
- National Institute of General Medical Sciences (GM64475, R01GM064475)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- nucleosome disassembly, PHO5 promoter, transcriptional activation, transcriptional repression, nucleosome reassembly, upstream activating sequence, Saccharomyces cerevisiae
- MeSH terms
- Acid Phosphatase, Binding Sites, Carrier Proteins, DNA-Binding Proteins, Histones, Models, Molecular, Nucleosomes, Promoter Regions, Genetic, Protein Binding, Repressor Proteins, Transcription Factors, Yeasts, Transcriptional Activation, Gene Expression Regulation, Fungal, Cell Cycle Proteins, Molecular Chaperones, Saccharomyces cerevisiae Proteins, Chromatin Assembly and Disassembly
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