Intranuclear Distribution and Local Dynamics of RNA Polymerase II during Transcription Activation
Open accessAbstract
Transcription activation causes dramatic changes in a gene's compaction and macromolecular associations and, in some cases, triggers the translocation of the gene to a nuclear substructure. Here, we evaluate the location, movement, and transcriptional dynamics of Drosophila heat shock (HS) genes both by two-photon microscopy in live polytene nuclei and by FISH in diploid nuclei. The different HS loci occupy separate nuclear positions. Although these loci decondense upon HS, they do not undergo a detectable net translocation nor are they preferentially localized to the nuclear periphery or interior. Additionally, fluorescence recovery after photobleaching reveals that, shortly after HS, newly recruited RNA polymerase II (Pol II) enters elongation via an "efficient entry" mode, which is followed by the progressive establishment of transcription "compartments" at Hsp70 loci where concentrated Pol II is used in a "local recycling" mode. Pol II at highly transcribed developmental loci exhibits dynamics resembling combinations of these Hsp70 transcription modes.
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FRAP with EGFP-tagged RNA Pol II on Hsp70 genes following heat shock in Drosophila calculated RNA Pol II speed between 1.1 and 1.5 kb/min.
“The authors calculated an RNA Pol II speed between 1.1 to 1.5 kb/min when measured on the Hsp70 genes following heat shock in Drosophila (Yao et al, 2007; Ardehali et al, 2009).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Yao et al, 2007
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References 47 · Cited by 111
- Authors
- Yao, J., Ardehali, M. B., Fecko, C. J., Webb, W. W., & Lis, J. T.
- Affiliations
- Cornell University (US)
- Corresponding author
- Webb, Watt W.
- Lis, John T.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2007-12
- Volume
- 28
- Issue
- 6
- Pages
- 978-990
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2010757862
- Cited by
- 111
- References
- 47
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 6 · 2018: 4 · 2017: 7 · 2016: 7 · 2015: 2 · 2014: 3 · 2013: 8 · 2012: 7
- FWCI
- 5.16
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- National Institute of General Medical Sciences (R01GM025232, GM-25232)
- National Institute of Biomedical Imaging and Bioengineering (P41EB001976)
- Division of Chemistry
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- RNA polymerase II, transcriptional activation, heat shock genes, HSP70, nuclear translocation, transcriptional dynamics, Drosophila, chromatin decondensation, nuclear periphery
- MeSH terms
- Animals, Cell Nucleus, Drosophila, Hot Temperature, Microscopy, Fluorescence, Recombinant Fusion Proteins, RNA Polymerase II, Time Factors, Transcriptional Activation, In Situ Hybridization, Fluorescence, HSP70 Heat-Shock Proteins, Protein Transport, Drosophila Proteins, Green Fluorescent Proteins
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