Priming and Extending: A UbcH5/Cdc34 E2 Handoff Mechanism for Polyubiquitination on a SCF Substrate

Wu, Kenneth, Kovacev, Jordan, Pan, Zhen-Qiang·2010·Molecular Cell

Cited by (126)

Items 101–126 of 126

  1. Monoubiquitination empowers ubiquitin chain elongation
    Kenneth Kun-yu Wu, Robert J. DeVita, Zhen‐Qiang Pan · 2024 · Journal of Biological Chemistry
  2. Design and Semisynthesis of Ubiquitin Extension Probes for Structural Analysis of Cullin1-Mediated Substrate Polyubiquitination
    Chuntong Li, Fangyu Zhao, Chong Zuo, Liying Zhang, Yangwode Jing, Xu Li, Guo‐Chao Chu, Luyu Shi, Yingyue Zhang, Han Wang et al. · 2025 · Journal of the American Chemical Society
  3. Synergistic effect of two E2 ubiquitin conjugating enzymes in SCFhFBH1 catalyzed polyubiquitination
    Jeong‐Hoon Kim, Jin Sun Choi, Sunhong Kim, Kidae Kim, Pyung Keun Myung, Sung Goo Park, Yeon‐Soo Seo, Byoung Chul Park · 2015 · BMB Reports
  4. Structure and dynamics of a pentameric KCTD5/Cullin3/Gβγ E3 ubiquitin ligase complex
    Duc Minh Nguyen, Deanna H. Rath, Dominic Devost, Darlaine Pétrin, Robert Rizk, Alan X. Ji, Naveen Narayanan, Darren Yong, Andrew Zhai, D.A. Kuntz et al. · 2023 · bioRxiv (Cold Spring Harbor Laboratory)
  5. A Modular Turn‐On Strategy to Profile E2‐Specific Ubiquitination Events in Living Cells
    Caitlin J. Hill, Suprama Datta, Nicholas P. McCurtin, Hannah Z. Kimball, Molly C. Kingsley, Abraham L. Bayer, Alexander C. Martin, Qianni Peng, Eranthie Weerapana, Rebecca A. Scheck · 2024 · Angewandte Chemie International Edition
  6. Diverse ubiquitin codes in the regulation of inflammatory signaling
    Fumiyo Ikeda · 2020 · Proceedings of the Japan Academy Series B
  7. Molecular characterization of the E2 conjugating enzyme LinfUbc13 in Leishmania infantum
    Eduardo Vagner Rodrigues da Silva, Caroline Torres, Hariel Nemamiah Escolarique Ribeiro, Camila Rolemberg Santana Travaglini Berti de Correia, Taissa de Oliveira de Castro, Giovanna da Costa Mancin, Mayla Gabriela Zanchetta Venancio, Munira Muhammad Abdel Baqui, Felipe R. Teixeira, Marcelo D. Gomes · 2024 · Archives of Biochemistry and Biophysics
  8. The Crosstalk Between CRL5 and APC/C E3 Ligases Regulates Metastasis and Chemosensitivity of Cancer Cells
    Danrui Cui, Ruirui Qu, Tianqi Li, Linchen Wang, Xiaoyu Chen, Shengpeng Shao, Xue Liang, Jun Xu, Yi Sun, Xiufang Xiong et al. · 2025 · Advanced Science
  9. Ubiquitin Proteasome System as Target for Tumor Therapy
    Mohamed Hassan Abdelouahid El · 2015 · Chemotherapy Open access
  10. A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer
    Kenneth Kun-yu Wu, Zhen‐Qiang Pan · 2021 · Methods in molecular biology
  11. Generation of a Proteolytic Signal: E3/E2-Mediated Polyubiquitination of IκBα
    Robert A. Chong, Kenneth Kun-yu Wu, Jordan Kovacev, Zhen‐Qiang Pan · 2015 · Methods in molecular biology
  12. Double Stranded DNA Breaks and Genome Editing Trigger Ribosome Remodeling and Translational Shutdown
    Celeste Riepe, Elena Zelin, Stacia K. Wyman, David N. Nguyen, Jin Rui Liang, Phillip Andrew Frankino, Zuriah A. Meacham, Jonathan T. Vu, Alexander Marson, Nicholas T. Ingolia et al. · 2018 · bioRxiv (Cold Spring Harbor Laboratory)
  13. Linker-rigidified VHL homodimerizers convert degraders into stabilizers of non-ubiquitinable ternary complexes
    Charlotte Crowe, Alessandra Salerno, Gajanan J. Sathe, Graham P. Marsh, Hannah J. Maple, Alessio Ciulli · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
  14. Functional and mechanistic characterization of the F-box protein Fbxw5
    Achim A Werner · 2011
  15. A Protein Engineering Approach for Uncovering Cryptic Ubiquitin-binding Sites: from a Ubiquitin-Variant Inhibitor of APC/C to K48 Chain Binding
    Edmond R. Watson, Christy R. Grace, Wei Zhang, Darcie J. Miller, Iain F. Davidson, Jesuraj Rajan Prabu, Shanshan Yu, Derek L. Bolhuis, Elizaveta T. Kulko, Ronnald Vollrath et al. · 2019 · bioRxiv (Cold Spring Harbor Laboratory)
  16. E3-mediated Ubiquitin and Ubiquitin-like Protein Ligation: Mechanisms and Chemical Probes
    David T. Krist, Brenda Arlene Schulman · 2020 · Royal Society of Chemistry eBooks
  17. SCF Fbxw5 targets MCAK in G 2 /M to facilitate ciliogenesis in the following cell cycle
    Jörg Schweiggert, Gregor Habeck, Sandra Hess, Felix Mikus, Klaus Meese, Shoji Hata, Klaus‐Peter Knobeloch, Frauke Melchior · 2021 · bioRxiv (Cold Spring Harbor Laboratory)
  18. Faculty Opinions recommendation of Ubiquitin chain-elongating enzyme UBE2S activates the RING E3 ligase APC/C for substrate priming.
    Hiroyuki Yamano, Kazuyuki Fujimitsu · 2021 · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
  19. An In vitro Assay to Recapitulate Hormone-Triggered and SCF-Mediated Protein Ubiquitylation
    Michael Niemeyer, Jhonny Oscar Figueroa Parra, Luz Irina A. Calderón Villalobos · 2022 · Methods in molecular biology
  20. A Modular Turn‐On Strategy to Profile E2‐Specific Ubiquitination Events in Living Cells
    Caitlin J. Hill, Suprama Datta, Nicholas P. McCurtin, Hannah Z. Kimball, Molly C. Kingsley, Abraham L. Bayer, Alexander C. Martin, Qianni Peng, Eranthie Weerapana, Rebecca A. Scheck · 2024 · Angewandte Chemie
  21. How post-translational modifications impact protein dynamics and stability—experimental and theoretical investigations
    Yuhao Li, Hailong Chen, Chun Tang · 2025 · Scientia Sinica Chimica
  22. Expression and purification of functional recombinant Cullin1-Rbx1/2 in E. coli
    Fangyu Zhao, Chuntong Li, Xu Li, Luyu Shi, Yingyue Zhang, Han Wang, Shuzhe Sun, Lujun Liang · 2025 · Protein Expression and Purification
  23. Single-Assay Characterization of Ternary Complex Assembly and Activity in Targeted Protein Degradation
    Corey H. Yu, Vi Dougherty, Dongwen Lv, Pirouz Ebadi, Shikha Dubey, Digant Nayak, Anindita Nayak, Daohong Zhou, Shaun K. Olsen, Dmitri N. Ivanov · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
  24. Induced ubiquitination of the partially disordered Estrogen Receptor alpha protein via a 14-3-3-directed molecular glue-based PROTAC design
    Carlo J. A. Verhoef, Charlotte Crowe, Mark A. Nakasone, Aitana de la Cuadra Basté, Tessa Harzing, Naomi A. S. Span, Gajanan J. Sathe, Kentaro Iso, Christian Ottmann, Luc Brunsveld et al. · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
  25. Systemic degradation of repressive transcription factors gates gene expression and cell fate specification
    Predrag Jevtić, Samuel R Witus, Devlon M. McCloud, Zhi Yang, Ana Milunovic-Jevtic, Heegwang Roh, Michael Rapé · 2026 · bioRxiv (Cold Spring Harbor Laboratory)
  26. A real-time FRET ubiquitin transfer assay for quantitative characterization of ternary complexes in targeted protein degradation
    Corey H. Yu, Vi Dougherty, Dongwen Lv, Pirouz Ebadi, Shikha Dubey, Digant Nayak, Anindita Nayak, Daohong Zhou, Shaun K. Olsen, Dmitri N. Ivanov · 2026 · Cell Reports Methods