Mapping the invisible chromatin transactions of prophase chromosome remodeling
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Abstract
SUMMARY We have used a combination of chemical genetics, chromatin proteomics and imaging to map the earliest chromatin transactions during vertebrate cell entry into mitosis. Chicken DT40 CDK1 as cells undergo synchronous mitotic entry within 15 minutes following release from a 1NM-PP1-induced arrest in late G 2 . In addition to changes in chromatin association with nuclear pores and the nuclear envelope, earliest prophase is dominated by changes in the association of ribonucleoproteins with chromatin, particularly in the nucleolus, where pre-rRNA processing factors leave chromatin significantly before RNA polymerase I. Nuclear envelope barrier function is lost early in prophase and cytoplasmic proteins begin to accumulate on the chromatin. As a result, outer kinetochore assembly appears complete by nuclear envelope breakdown (NEBD). Most interphase chromatin proteins remain associated with chromatin until NEBD, after which their levels drop sharply. An interactive proteomic map of chromatin transactions during mitotic entry is available as a resource at https://mitoChEP.bio.ed.ac.uk .
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Cites
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BackgroundInfluentialOrganization of the mitotic chromosome
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- Authors
- Samejima, I., Spanos, C., Samejima, K., Rappsilber, J., Kustatscher, G., & Earnshaw, W. C.
- Affiliations
- Wellcome Centre for Cell Biology (GB)
- University of Edinburgh (GB)
- Corresponding author
- Kustatscher, Georg
- Earnshaw, William C.
- ORCID
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2022-02
- Volume
- 82
- Issue
- 3
- Pages
- 696-708.e4
- ISSN
- 1097-2765 (ISSN-L)
- PMC
- PMC8823707
- OpenAlex
- W4210330911
- Cited by
- 21
- References
- 4
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 7 · 2023: 4 · 2022: 2
- FWCI
- 1.55
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Edinburgh Research Explorer (University of Edinburgh) (publishedVersion, cc-by)
- Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, cc-by)
- Funders
- Wellcome Trust (203149, 107022, 221044)
- Medical Research Council (MR/T03050X/1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- mitotic entry, CDK1, nuclear envelope breakdown, ribonucleoprotein, nucleolus, pre-rRNA processing, nuclear pore complex, chromatin proteomics, DT40 cells, G2 cell cycle arrest
- MeSH terms
- Animals, Chickens, Chromatin, Chromosomes, DNA, Nuclear Proteins, Prophase, Protein Binding, RNA, Ribosomal, Time Factors, CDC2 Protein Kinase, Lymphoma, B-Cell, Proteome, Lamin Type B, Proteomics, Chromatin Assembly and Disassembly, Cell Line, Tumor
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