(Chitosan-g-glycidyl methacrylate)-xanthan hydrogel implant in Wistar rats for spinal cord regeneration
Open accessAbstract
This work reports the results of in vivo assays of an implant composed of the hydrogel Chitosan-g-Glycidyl Methacrylate-Xanthan [(CTS-g-GMA)-X] in Wistar rats. Degradation kinetics of hydrogels was assessed by lysozyme assays. Wistar rats were subjected to laminectomy by cutting the spinal cord with a scalpel. After the surgical procedure, hydrogels were implanted in the injured zone (level T8). Somatosensory evoked potentials (SEPs) obtained by electric stimulation onto periphery nerves were registered in the corresponding central nervous system (CNS) areas. Rats implanted with the biomaterials showed a successful recovery compared with the non-implanted rats after 30days. Lysozyme, derived from egg whites, was used for in vitro assays. This study serves as the basis for testing the biodegradability of the hydrogels (CTS-g-GMA)-X that is promoted by enzymatic hydrolysis. Hydrogels' hydrolysis was studied via lysozyme kinetics at two pH values, 5 and 7, under mechanical agitation at 37°C. Results show that our materials' hydrolysis is slower than pure CTS possibly due to the steric hindrance imposed by the GMA grafting of functionalization. This hydrolysis helps degrade the biomaterial and at the same time it provides support for spinal cord recovery. Combination of these results may prove useful in the use of these hydrogels as scaffolds for cells proliferation and their application as implants in living organisms.
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In spinal cord repair, freeze-drying technology has made it possible to produce porous structures quickly and easily.
“In the realm of spinal cord repair, freeze-drying technology has made it possible to produce porous structures quickly and easily [69].”
From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [69]
The available indexed passages identify the paper as a study of a hydrogel implant for spinal cord regeneration, but contain no discussion of how porous structures were produced. Searches for freeze-drying, lyophilization, and porous hydrogel preparation returned only bibliographic material, so the claimed ease and speed of production cannot be verified from our copy. (LLM, conf=0.72)
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Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review
Vach Agocsova, S., Culenova, M., Birova, I., Omanikova, L., Moncmanova, B., Danisovic, L., Ziaran, S., Bakos, D., & Alexy, P. (2023) · Materials · doi:10.3390/ma16124267 · Cited by 98 · see citing works
- Authors
- Elizalde-Peña, E. A., Quintero-Ortega, I. A., Zárate-Triviño, D. G., Nuño-Licona, A., Gough, J., Sanchez, I. C., Medina, D. I., & Luna-Barcenas, G.
- Affiliations
- Autonomous University of Queretaro (MX)
- Universidad Autónoma de Nuevo León (MX)
- Instituto Nacional de Rehabilitación (MX)
- University of Manchester (GB)
- Tecnológico de Monterrey (MX)
- Corresponding author
- Medina, D.I.
- Venue
- Materials Science and Engineering: C
- Publisher
- Elsevier BV
- Published
- 2017-09
- Volume
- 78
- Pages
- 892-900
- ISSN
- 0928-4931 (ISSN-L)
- OpenAlex
- W2594922148
- Cited by
- 20
- References
- 0
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 4 · 2019: 3 · 2018: 1 · 2017: 1
- FWCI
- 0.95
- Type
- journal article
- Language
- en
- Open access
- Licence
- cc-by-nc-nd
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- Materials Science and Engineering C (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (publishedVersion, cc-by-nc-nd)
- Funders
- CONACYT Mexico (181678)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Health Sciences › Medicine › Pathology and Forensic Medicine › Spinal Cord Injury Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Keywords
- xanthan gum, hydrogel implants, spinal cord regeneration, spinal cord injury, hydrogel degradation, enzymatic hydrolysis, somatosensory evoked potentials, grafting density, scaffolds, cell proliferation
- MeSH terms
- Animals, Polymethacrylic Acids, Polysaccharides, Bacterial, Spinal Cord, Rats, Wistar, Hydrogels, Chitosan, Rats, Spinal Cord Regeneration
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