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Cited by 63Fungal Plant Pathogen ControlPlant Pathogens and Fungal DiseasesPlant Disease Resistance and Genetics

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Specific detection of benzimidazole resistance in Colletotrichum gloeosporioides from fruit crops by PCR-RFLP

Chung, Wen-Hsin, Chung, Wen-Chuan, Peng, Mun-Tsu, Yang, Hong-Ren, Huang, Jenn-Wen·2010·New Biotechnology
DOI 10.1016/j.nbt.2009.10.004
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Abstract

Anthracnose diseases, caused by Colletotrichum gloeosporioides, are a worldwide problem and are especially important in Taiwan owing to the severe economic damage they cause to tropical fruits that are grown for local consumption and export. Benzimidazoles are systemic fungicides widely used for controlling these diseases in Taiwan. Thirty-one isolates of C. gloeosporioides from mango and strawberry grown in Taiwan were examined for their sensitivity to benzimidazole fungicides. The responses of the isolates grown on benzimidazole-amended culture media were characterized as sensitive, moderately resistant, resistant or highly resistant. Analysis of point mutations in the beta-tubulin gene by DNA sequencing of PCR-amplified fragments revealed a substitution of GCG for GAG at codon 198 in resistant and highly resistant isolates and a substitution of TAC for TTC at codon 200 in moderately resistant isolates. A set of specific primers, TubGF1 and TubGR, was designed to amplify a portion of the beta-tubulin gene for the detection of benzimidazole-resistant C. gloeosporioides. Bsh1236I restriction maps of the amplified beta-tubulin gene showed that the resistant isolate sequence, but not the sensitive isolate sequence, was cut. The PCR restriction fragment length polymorphism (PCR-RFLP) was validated to detect benzimidazole-resistant and benzimidazole-sensitive C. gloeosporioides isolates recovered from avocado, banana, carambola, dragon fruit, grape, guava, jujube, lychee, papaya, passion fruit and wax apple. This method has the potential to become a valuable tool for monitoring the occurrence of benzimidazole-resistant C. gloeosporioides and for assessment of the need for alternative management practices.

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  1. Davidse, 1986, Benzimidazole fungicides: mechanism of action and biological impact, Annu. Rev. Phytopahtol., 24, 43, 10.1146/annurev.py.24.090186.000355 · DOI
  2. Koenraadt, 1992, The use of allele-specific oligonucleotide probes to characterize resistance to benomyl in filed strains of Venturia inqeualis, Phytopathology, 82, 1354, 10.1094/Phyto-82-1354 · DOI
  3. Luck, 1995, Rapid identification of benomyl resistant strains of Botrytis cinerea using the polymerase chain reaction, Mycol. Res., 99, 1483, 10.1016/S0953-7562(09)80797-1 · DOI
  4. Yarden, 1993, Mutations leading to substitutions at amino acids 198 and 200 of beta-tubulin that correlate with benomyl-resistance phenotypes of field strains of Botrytis cinerea, Phytopathology, 83, 1478, 10.1094/Phyto-83-1478 · DOI
  5. Albertini, 1999, Mutations of the β-tubulin gene associated with different phenotypes of benzimidazole resistance in the cereal eyespot fungi Tapesia yallundae and Tapesia acuformis, Pestic. Biochem. Physiol., 64, 17, 10.1006/pest.1999.2406 · DOI
  6. Ma, 2003, Identification and characterization of benzimidazole resistance in Monilinia fructicola from stone fruit orchards in California, Appl. Environ. Microbiol., 69, 7145, 10.1128/AEM.69.12.7145-7152.2003 · DOI
  7. Chung, 2006, Fungicide sensitivity and phylogenetic relationship of anthracnose fungi isolated from various fruit crops in Japan, Plant Dis., 90, 506, 10.1094/PD-90-0506 · DOI
  8. Kim, 2007, Analyses of the less benzimidazole-sensitivity of the isolates of Colletotrichum spp. causing the anthracnose in pepper and strawberry, Plant Pathol. J., 23, 187, 10.5423/PPJ.2007.23.3.187 · DOI
  9. Yano, 2004, Anthracnose on Japanese pear caused by intermediately benzimidazole resistant strains of Colletotrichum gloeosporioides (Glomerella cingulata), Jpn. J. Phytopathol., 70, 314, 10.3186/jjphytopath.70.314 · DOI
  10. Zhan, 2007, Cloning of a carbendazim-resistant gene from Colletotrichum gloeosporioides of mango in South China, Afr. J. Biotech., 6, 143
  11. Cañas-Gutiérrez, 2006, Molecular characterization of benomyl-resistant isolates of Mycosphaerella fjijensis, collected in Colombia, J. Phytopathol., 154, 403, 10.1111/j.1439-0434.2006.01113.x · DOI
  12. Cooley, 1993, Molecular analysis of the Septoria nodorum β-tubulin gene and characterization of a benomyl-resistant mutation, Mol. Gene Genet., 237, 58, 10.1007/BF00282784 · DOI
  13. Jung, 1990, Identification of an amino acid substitution in the benA, β-tubulin gene of Aspergillus nidulans that confers thiabendazole resistance and benomyl supersensitivity, Cell Motil. Cytoskeleton, 17, 87, 10.1002/cm.970170204 · DOI
  14. Jung, 1992, Amino acid alterations in the benA (beta-tubulin) gene of Aspergillus nidulans that confers benomyl resistance, Cell Motil. Cytoskeleton, 22, 170, 10.1002/cm.970220304 · DOI
  15. Orbach, 1986, Cloning and characterization of the gene for β-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker, Mol. Cell. Biol., 6, 2452
  16. Nakaune, 2007, Benomyl resistance of Colletotrichum acutatum is caused by enhanced expression of β-tubulin 1 gene regulated by putative leucine zipper protein CaBEN1, Fungal Genet. Biol., 44, 1324, 10.1016/j.fgb.2007.03.007 · DOI
  17. Pei, C.L. (1981) Investigation on resistance of phytopathogenic fungi to fungicides in Taiwan. Master Thesis, National Chung Hsing University
  18. Tsai, 2006, Evaluation of fungicides for suppression of mycelial growth and conidial germination of Colletotrichum species isolated from mango, pomelo and banana fruit, Plant Pathol. Bull., 15, 39
  19. Thompson, 1997, The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools, Nucleic Acid Res., 25, 4876, 10.1093/nar/25.24.4876 · DOI
  20. Vargas, 1973, A benzimidazole resistant strain of Erysiphe graminis, Phytopathology, 63, 1366, 10.1094/Phyto-63-1366 · DOI
  21. Bollen, 1971, Acquired resistance to benomyl and some other systemic fungicides in a strain of Botrytis cinerea in cyclamen, Neth. J. Plant Pathol., 77, 83, 10.1007/BF01981496 · DOI
  22. Iida, 1975, On the tolerance of plant pathogenic fungi and bacteria to fungicides in Japan, Jpn. Pest. Information, 23, 13
  23. Jarvis, 1973, Tolerance to benomyl in Botrytis cinerea and Penicillium corymbiferum, Plant Pathol., 22, 139, 10.1111/j.1365-3059.1973.tb01792.x · DOI
  24. Wicks, 1977, Tolerance to benzimidazole fungicides in blue mold (Penicillium expansum) on pears, Plant Dis. Rep., 61, 447
  25. Wild, 1983, Double resistance by citrus green mold Penicillium digitatum to the fungicides guazatine and benomyl, Ann. Appl. Biol., 103, 237, 10.1111/j.1744-7348.1983.tb02760.x · DOI
  26. Koenraadt, 1992, Characterization of mutations in the beta-tubulin gene of benomyl-resistant field strains of Venturia inaequalis and other plant pathogenic fungi, Phytopathology, 82, 1348, 10.1094/Phyto-82-1348 · DOI
  27. Stover, 1979, Field observations on benomyl tolerance in ascospores of Mycosphaerella fijiensis var. difformis, Trans. British Mycol. Soc., 72, 518, 10.1016/S0007-1536(79)80172-2 · DOI
  28. Thomas, 1985, Isolation and characterization of mutations in the β-tubulin gene of Saccharomyces cerevisiae, Genetics, 112, 715, 10.1093/genetics/111.4.715 · DOI

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Metadata

Authors
Chung, W.-H., Chung, W.-C., Peng, M.-T., Yang, H.-R., & Huang, J.-W.
Affiliations
  • National Chung Hsing University (TW)
  • Taiwan Agricultural Research Institute Council of Agriculture, Executive Yuan (TW)
Corresponding author
Huang, Jenn-Wen
ORCID
Venue
New Biotechnology
Publisher
Elsevier BV
Published
2010-02
Volume
27
Issue
1
Pages
17-24
ISSN
1871-6784 (ISSN-L)
DOI
10.1016/j.nbt.2009.10.004
OpenAlex
W2081925087
Cited by
63
References
2
Citations by year
2026: 3 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 6 · 2021: 4 · 2020: 10 · 2019: 4 · 2018: 6 · 2017: 2 · 2016: 3 · 2015: 2 · 2014: 4 · 2013: 2 · 2012: 2
FWCI
1.25
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • New Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Education
  • Council of Agriculture
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Fungal Plant Pathogen Control
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogens and Fungal Diseases
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Disease Resistance and Genetics
Keywords
Colletotrichum gloeosporioides, benzimidazole resistance, anthracnose, beta-tubulin gene, point mutations, PCR-RFLP, mango, strawberry, tropical fruit crops, fungicide resistance monitoring
MeSH terms
Benzimidazoles, Fruit, Fungicides, Industrial, Microbial Sensitivity Tests, Plant Diseases, Polymorphism, Restriction Fragment Length, Taiwan, Tubulin, Polymerase Chain Reaction, Point Mutation, Crops, Agricultural, Colletotrichum, Drug Resistance, Fungal

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CoChat. (2026). Citation check: Specific detection of benzimidazole resistance in Colletotrichum gloeosporioides from fruit crops by PCR-RFLP. https://app.cochat.ai/papers/10.1016/j.nbt.2009.10.004
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Chung, W.-H., Chung, W.-C., Peng, M.-T., Yang, H.-R., & Huang, J.-W. (2010). Specific detection of benzimidazole resistance in Colletotrichum gloeosporioides from fruit crops by PCR-RFLP. New Biotechnology. https://doi.org/10.1016/j.nbt.2009.10.004