Upregulation of Lhx8 increase VAChT expression and ACh release in neuronal cell line SHSY5Y
Abstract
Lhx8 is a transcription factor for cholinergic differentiation. Our previous experiments found upregulation of Lhx8 promoted cholinergic neuronal differentiation of hippocampal neural stem/progenitor cells or hippocampal newborn neurons in vitro. However, the role of Lhx8 in VAChT expression and ACh release is still less understood. In this report, we transfected Lhx8 cDNA into neuronal cell line SHSY5Y by lentiviral vectors to acquire the cells which stably expressed high level of Lhx8. Using this cell model, we provided experimental evidence that increasing Lhx8 upregulated the expression of ChAT and VAChT, and also increased the ACh release in culture medium. We suggested that Lhx8 overexpression is a useful strategy to increase the release of ACh and maybe of therapeutic value to neurodegenerative diseases.
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Lhx8 expression induces vesicular ACh transporter activity and acetylcholine release.
“Lhx8 expression also induces Vesicular ACh transporter activity and acetylcholine release [54,55].”
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Milewski, T. M., Lee, W., Young, R. L., Hofmann, H. A., & Curley, J. P. (2025) · PLoS Genetics · doi:10.1371/journal.pgen.1011548 · Cited by 7 · see citing works
- Authors
- Li, H., Jin, G., Zhu, P., Zou, L., Shi, J., Yi, X., Zhang, X., Tian, M., & Qin, J.
- Affiliations
- Nantong University (CN)
- Shanghai Changzheng Hospital (CN)
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2014-01
- Volume
- 559
- Pages
- 184-188
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2065558069
- Cited by
- 8
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2022: 1 · 2021: 1 · 2019: 1 · 2015: 1 · 2014: 1
- FWCI
- 0.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31070937)
- Natural Science Foundation of Jiangsu Higher Education Institutions (13KJB310011)
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
- Keywords
- Lhx8, vesicular acetylcholine transporter, acetylcholine release, cholinergic differentiation, choline acetyltransferase, SH-SY5Y cells, lentiviral overexpression, neurodegenerative diseases
- MeSH terms
- Acetylcholine, Gene Expression Regulation, Humans, Neurons, Random Allocation, Transcription Factors, Up-Regulation, Cell Line, Tumor, Vesicular Acetylcholine Transport Proteins, LIM-Homeodomain Proteins
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