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Cited by 136Cellular transport and secretionNeuroscience and Neuropharmacology ResearchExtracellular vesicles in disease

Mutations in Drosophila sec15 Reveal a Function in Neuronal Targeting for a Subset of Exocyst Components

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Mehta, Sunil Q., Hiesinger, P. Robin, Beronja, Slobodan, Zhai, R. Grace, Schulze, Karen L., Verstreken, Patrik, Cao, Yu, Zhou, Yi +3 more·2005·Neuron
DOI 10.1016/j.neuron.2005.02.029
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Abstract

The exocyst is a complex of proteins originally identified in yeast that has been implicated in polarized secretion. Components of the exocyst have been implicated in neurite outgrowth, cell polarity, and cell viability. We have isolated an exocyst component, sec15, in a screen for genes required for synaptic specificity. Loss of sec15 causes a targeting defect of photoreceptors that coincides with mislocalization of specific cell adhesion and signaling molecules. Additionally, sec15 mutant neurons fail to localize other exocyst members like Sec5 and Sec8, but not Sec6, to neuronal terminals. However, loss of sec15 does not cause cell lethality in contrast to loss of sec5 or sec6. Our data suggest a role of Sec15 in an exocyst-like subcomplex for the targeting and subcellular distribution of specific proteins. The data also show that functions of other exocyst components persist in the absence of sec15, suggesting that different exocyst components have separable functions.

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  • The distribution of the exocyst within a cell can be highly dynamic, consistent with its role in directing membrane fusion to specific domains.

    “The distribution of the exocyst within a cell can be highly dynamic, consistent with its role in directing membrane fusion to specific domains (Boyd et al, 2004;Beronja et al, 2005;Mehta et al, 2005;Zhang et al, 2008;Murthy et al, 2010).”

    From Ral mediates activity‐dependent growth of postsynaptic membranes via recruitment of the exocyst (2013) · cites this paper as Mehta et al, 2005

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Authors
Mehta, S. Q., Hiesinger, P. R., Beronja, S., Zhai, R. G., Schulze, K. L., Verstreken, P., Cao, Y., Zhou, Y., Tepass, U., Crair, M. C., & Bellen, H. J.
Affiliations
  • Baylor College of Medicine (US)
  • Howard Hughes Medical Institute (US)
  • University of Toronto (CA)
Corresponding author
Bellen, Hugo J.
ORCID
Venue
Neuron
Publisher
Elsevier BV
Published
2005-04
Volume
46
Issue
2
Pages
219-232
ISSN
0896-6273 (ISSN-L)
DOI
10.1016/j.neuron.2005.02.029
OpenAlex
W2107396888
Cited by
136
References
0
Citations by year
2025: 1 · 2024: 3 · 2023: 5 · 2022: 1 · 2021: 4 · 2020: 5 · 2019: 5 · 2018: 3 · 2017: 3 · 2016: 7 · 2015: 8 · 2014: 7 · 2013: 8 · 2012: 10
FWCI
4.18
Type
journal article
Language
en
Open access
Licence
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
  • Lirias (submittedVersion)
Funders
  • National Institute of Mental Health (MH62639, R01MH062639)
  • National Eye Institute (EY-07001, R01EY015788)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
exocyst, Sec15, Sec5, Sec8, Sec6, synaptic specificity, neuronal targeting, photoreceptors, polarized secretion, adhesion molecules, signaling molecules, subcellular localization, Drosophila
MeSH terms
Amino Acid Sequence, Animals, Drosophila, Exocytosis, Humans, Immunohistochemistry, Membrane Proteins, Molecular Sequence Data, Mutation, Neurons, Synapses, Blotting, Western, Polymerase Chain Reaction, Sequence Homology, Amino Acid, Protein Transport, Microscopy, Electron, Transmission

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