Merits and Limitations of Vesicle Pool Models in View of Heterogeneous Populations of Synaptic Vesicles
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The concept of a readily releasable pool (RRP) of synaptic vesicles has been used extensively for the analysis of neurotransmitter release. Traditionally the properties of vesicles in such a pool have been assumed to be homogeneous, and techniques have been developed to determine pool parameters, such as the size of the pool and the probability with which a vesicle is released during an action potential. Increasing evidence, however, indicates that vesicles may be quite heterogeneous with respect to their release probability. The question, therefore, arises: what do the estimates of pool parameters mean in view of such heterogeneity? Here, four methods for obtaining pool estimates are reviewed, together with their underlying assumptions. The consequences of violation of these assumptions are discussed, and how apparent pool sizes are influenced by stimulation strength is explored by simulations.
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The cumulative EPSC method can distinguish between changes in RRP and Pr, although the resulting RRP values represent pool decrement rather than absolute pool size.
“This method can distinguish between changes in RRP and Pr, although the resulting RRP values represent “pool decrement” rather than absolute pool size ([11]; see Materials and methods).”
From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [11]
Biophysical studies often delineate different vesicle pools at presynaptic active zones, but how these pools correlate to vesicle populations in electron micrographs or tomograms remains unclear.
“Finally, biophysical studies often delineate different vesicle pools at presynaptic active zones (AZs), specialized sites of synaptic vesicles fusion and neurotransmitter release [9–13] but how these pools correlate to vesicle populations in electron micrographs or tomograms remains unclear.”
From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [11]
RRP estimates obtained by the cumulative EPSC peak amplitude method represent pool decrement rather than absolute pool size.
“Note that the RRP estimates by this method represent “pool decrement” rather than absolute pool size [11].”
From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [11]
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References 49 · Cited by 236
- Authors
- Neher, E.
- Affiliations
- Max Planck Institute for Biophysical Chemistry (DE)
- Corresponding author
- Neher, Erwin
- ORCID
- Neher, Erwin
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2015-09
- Volume
- 87
- Issue
- 6
- Pages
- 1131-1142
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2125752525
- Cited by
- 236
- References
- 49
- Citations by year
- 2026: 11 · 2025: 17 · 2024: 26 · 2023: 22 · 2022: 18 · 2021: 19 · 2020: 21 · 2019: 33 · 2018: 22 · 2017: 23 · 2016: 22 · 2015: 2
- FWCI
- 2.77
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Digital Library (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- readily releasable pool, synaptic vesicles, vesicle heterogeneity, release probability, neurotransmitter release, action potential
- MeSH terms
- Action Potentials, Animals, Humans, Models, Biological, Synaptic Transmission, Synapses, Synaptic Vesicles
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