The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.
Dendritic spines disappear with chilling but proliferate excessively upon rewarming of mature hippocampus
Abstract
More dendritic spine synapses occur on mature neurons in hippocampal slices by 2 h of incubation in vitro, than in perfusion-fixed hippocampus. What conditions initiate this spinogenesis and how rapidly do the spines begin to proliferate on mature neurons? To address these questions, CA1 field of the hippocampus neurons expressing green fluorescent protein in living slices from mature mice were imaged with two-photon microscopy. Spines disappeared and dendrites were varicose immediately after slice preparation in ice-cold artificial cerebrospinal fluid (ACSF). Electron microscopy (EM) revealed disrupted dendritic cytoplasm, enlarged or free-floating postsynaptic densities, and excessive axonal endocytosis. Upon warming dendritic varicosities shrank and spines rapidly reappeared within a few minutes illustrating the remarkable resilience of mature hippocampal neurons in slices. When membrane impermeant sucrose was substituted for NaCl in ACSF dendrites remained spiny at ice-cold temperatures and EM revealed less disruption. Nevertheless, spine number and length increased within 30 min in warm ACSF even when the extracellular calcium concentration was zero and synaptic transmission was blocked. When slices were first recovered for several hours and then chilled in 6 degrees C ACSF many spines disappeared and the dendrites became varicose. Upon re-warming varicosities shrank and spines reemerged in the same position from which they disappeared. In addition, new spines formed and…
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Data from investigators using in vitro ischemia, tissue cooling, and epilepsy support findings that spines can be reversibly altered (Hasbani et al., 2001;Kirov et al., 2004;Zeng et al., 2007).”
From Two-Photon Imaging of Stroke Onset In Vivo Reveals That NMDA-Receptor Independent Ischemic Depolarization Is the Major Cause of Rapid Reversible Damage to Dendrites and Spines (2008) · cites this paper as Kirov et al., 2004
In the citing paper
“In the case of ion imbalance brought about by brain slicing in vitro, a similar phenomenon occurs, and the recovered dendrites are apparently functional because synaptic transmission is observed (Hasbani et al., 2001;Kirov et al., 2004).”
From Rapid Reversible Changes in Dendritic Spine Structure In Vivo Gated by the Degree of Ischemia (2005) · cites this paper as Kirov et al., 2004
In the citing paper
“The cytoskeleton appears to be quite resilient in vivo, because reperfusion is associated with a remarkable reversal of swollen spines and their dendrites toward normal structure, consistent with work done in vitro (Hasbani et al., 2001;Kirov et al., 2004).”
From Rapid Reversible Changes in Dendritic Spine Structure In Vivo Gated by the Degree of Ischemia (2005) · cites this paper as Kirov et al., 2004
In the citing paper
“These in vitro findings suggest that the presynaptic terminal is still attached during the reversible dendritic swelling.”
From Rapid Reversible Changes in Dendritic Spine Structure In Vivo Gated by the Degree of Ischemia (2005) · cites this paper as Kirov et al., 2004
Citations
Cites
An Energy Budget for Signaling in the Grey Matter of the Brain
Attwell, D., & Laughlin, S. B. (2001) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/00004647-200110000-00001 · Cited by 3,543
Synaptic Activity and the Construction of Cortical Circuits
Katz, L. C., & Shatz, C. J. (1996) · Science · doi:10.1126/science.274.5290.1133 · Cited by 3,048
Ischemic Cell Death in Brain Neurons
Lipton, P. (1999) · Physiological Reviews · doi:10.1152/physrev.1999.79.4.1431 · Cited by 3,043
Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
Feng, G., Mellor, R. H., Bernstein, M., Keller-Peck, C., Nguyen, Q. T., Wallace, M., Nerbonne, J. M., Lichtman, J. W., & Sanes, J. R. (2000) · Neuron · doi:10.1016/s0896-6273(00)00084-2 · Cited by 3,042
Gautier, J. (1993) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(93)90025-i · Cited by 2,200
Longitudinal Assessment of Neurocognitive Function after Coronary-Artery Bypass Surgery
Newman, M. F., Kirchner, J., Phillips‐Bute, B., Gaver, V., Grocott, H. P., Jones, R. H., Mark, D. B., Reves, J. G., & Blumenthal, J. A. (2001) · New England Journal of Medicine · doi:10.1056/nejm200102083440601 · Cited by 1,963
Axo-somatic and axo-dendritic synapses of the cerebral cortex: an electron microscope study.
Eg, G. (1959) · PubMed · Cited by 1,768
Harris, K. M., & Kater, S. B. (1994) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.17.030194.002013 · Cited by 1,020
Self-citationEvidence for a Role of Dendritic Filopodia in Synaptogenesis and Spine Formation
Ziv, N. E., & Smith, S. J. (1996) · Neuron · doi:10.1016/s0896-6273(00)80283-4 · Cited by 882
Synaptogenesis Via Dendritic Filopodia in Developing Hippocampal Area CA1
Fiala, J. C., Feinberg, M., Popov, V., & Harris, K. M. (1998) · The Journal of Neuroscience · doi:10.1523/jneurosci.18-21-08900.1998 · Cited by 758
Self-citation
Research works printed but not linked: 1
- Gray, 1959, Axo-somatic and axo-dendritic synapses of the cerebral cortex, J Anat, 93, 420
Cited by
Sorted by citations
Balancing Structure and Function at Hippocampal Dendritic Spines
Bourne, J. N., & Harris, K. M. (2008) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.31.060407.125646 · Cited by 916
Self-citationReconstruct : a free editor for serial section microscopy
Fiala, J. C. (2005) · Journal of Microscopy · doi:10.1111/j.1365-2818.2005.01466.x · Cited by 889
Self-citationThe Subspine Organization of Actin Fibers Regulates the Structure and Plasticity of Dendritic Spines
Honkura, N., Matsuzaki, M., Noguchi, J., Ellis-Davies, G. C. R., & Kasai, H. (2008) · Neuron · doi:10.1016/j.neuron.2008.01.013 · Cited by 490
Dendritic spines and long-term plasticity
Segal, M. (2005) · Nature Reviews Neuroscience · doi:10.1038/nrn1649 · Cited by 489
Dendritic Spines: The Locus of Structural and Functional Plasticity
Sala, C., & Segal, M. (2014) · Physiological Reviews · doi:10.1152/physrev.00012.2013 · Cited by 491
Metadata
- Authors
- Kirov, S. A., Petrak, L. J., Fiala, J. C., & Harris, K. M.
- Affiliations
- Augusta University (US)
- Boston University (US)
- Corresponding author
- Kirov, S.A
- Venue
- Neuroscience
- Publisher
- Elsevier BV
- Published
- 2004-01
- Volume
- 127
- Issue
- 1
- Pages
- 69-80
- ISSN
- 0306-4522 (ISSN-L)
- OpenAlex
- W2126033680
- Cited by
- 172
- References
- 56
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 5 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 5 · 2019: 3 · 2018: 2 · 2017: 7 · 2016: 7 · 2015: 7 · 2014: 12 · 2013: 6 · 2012: 12
- FWCI
- 5.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Mental Health (K01 MH 02000, K01MH002000)
- National Institute of Neurological Disorders and Stroke (R01NS033574, NS21184, NS33574)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- dendritic spines, spine plasticity, spinogenesis, CA1 pyramidal neurons, rewarming, dendritic varicosities, postsynaptic density, sucrose replacement, neuronal recovery, ion homeostasis, two-photon imaging
- MeSH terms
- Animals, Calcium, Cerebrospinal Fluid, Cold Temperature, Dendrites, Hippocampus, Homeostasis, Male, Mice, Transgenic, Microscopy, Electron, Synaptic Transmission, Neuronal Plasticity, Sucrose, Synapses, Synaptic Membranes, Temperature, Water-Electrolyte Balance, Cell Size, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
