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Oxidative Stress-mediated Sprouty-related Protein with an EVH1 Domain 1 Down-regulation Contributes to Resisting Oxidative Injury in Microglia

Wei, Li, Li, Xin, Wei, Qianfeng, Chen, Lin, Xu, Li, Zhou, Peng·2023·Neuroscience
DOI 10.1016/j.neuroscience.2023.06.012
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Fig. 1 ( A ) TTC-stained mice brain slice after experimental stroke (MCAO, 60 min occlusion, 24 h reperfusion). ( B ) The expression levels of eNOS, iNOS, SOD1, SOD2 after MCAO treatment in cerebral cortex. GAPDH was used as a loading control. ( C ) Quantification of the malondialdehyde (MDA) in cerebral cortex after MCAO treatment. ( D, E ) The activities of superoxide dismutase (SOD) and catalase were detected in cerebral cortex after MCAO treatment. (*, p < 0.05, n = 5) ( F ) The representative images of immune-histochemical assays for SPRED1 expression in cerebral cortex. ( G ) The expression levels of SPRED1 after MCAO treatment in cerebral cortex. GAPDH was used as a loading control. ( H ) Quantification of the SPRED1 protein level after MCAO treatment. (*, p <0.05).
Fig. 1 ( A ) TTC-stained mice brain slice after experimental stroke (MCAO, 60 min occlusion, 24 h reperfusion). ( B ) The expression levels of eNOS, iNOS, SOD1, SOD2 after MCAO treatment in cerebral cortex. GAPDH was used as a loading control. ( C ) Quantification of the malondialdehyde (MDA) in cerebral cortex after MCAO treatment. ( D, E ) The activities of superoxide dismutase (SOD) and catalase were detected in cerebral cortex after MCAO treatment. (*, p < 0.05, n = 5) ( F ) The representative images of immune-histochemical assays for SPRED1 expression in cerebral cortex. ( G ) The expression levels of SPRED1 after MCAO treatment in cerebral cortex. GAPDH was used as a loading control. ( H ) Quantification of the SPRED1 protein level after MCAO treatment. (*, p <0.05).CC-BY-NC-ND · From the PDF
Fig. 2 ( A ) Schematic representation of the domains of SPRED1 of human origin. ( B, C ) Detection of the level of SPRED1 protein in BV2 cells after 100 l M H2O2 treatment for 1, 3, 6, 12 and 24 h, and quantified in the right (C). GAPDH was used as a loading control. ( D-F ) Detection of the levels of p38, p65, SOD1 and SOD2 proteins in BV2 cells after 100 l M H2O2 treatment for 1, 3, 6, 12 and 24 h, quantified in the right (E) and below (F). b -actin was used as a loading control.
Fig. 2 ( A ) Schematic representation of the domains of SPRED1 of human origin. ( B, C ) Detection of the level of SPRED1 protein in BV2 cells after 100 l M H2O2 treatment for 1, 3, 6, 12 and 24 h, and quantified in the right (C). GAPDH was used as a loading control. ( D-F ) Detection of the levels of p38, p65, SOD1 and SOD2 proteins in BV2 cells after 100 l M H2O2 treatment for 1, 3, 6, 12 and 24 h, quantified in the right (E) and below (F). b -actin was used as a loading control.CC-BY-NC-ND · From the PDF
Fig. 3 ( A, B ) Detection of the levels of SPRED1, p38 and p65 proteins in BV2 cells after transfected by SPRED1 plasmid along with the treatment of H2O2, and quantified their levels in ( B ). GAPDH was used as the loading control. ( C, D ) Detect the levels of TNFa and IL-1 b via ELISA kits in BV2 cells after treatment as the procedures in ( A, B ) for 24 h. (*, p <0.05).
Fig. 3 ( A, B ) Detection of the levels of SPRED1, p38 and p65 proteins in BV2 cells after transfected by SPRED1 plasmid along with the treatment of H2O2, and quantified their levels in ( B ). GAPDH was used as the loading control. ( C, D ) Detect the levels of TNFa and IL-1 b via ELISA kits in BV2 cells after treatment as the procedures in ( A, B ) for 24 h. (*, p <0.05).CC-BY-NC-ND · From the PDF
Fig. 4 ( A ) The relative value of CCK-8 in BV2 cells after transfection with H2O2 or/and pcDNA3.1-SPRED1 for 24 h. ( B ) Flow cytometer was used to detect the fluorescence intensity of PI-labeled and FITC-labeled after transfection. ( C ) According to streaming results, the average fluorescence intensity of each group was counted. ( D, E ) Detection of the levels of Bax and Bcl2 proteins in BV2 cells after transfection, quantified in the right (E). GAPDH was used as a loading control. (*, p <0.05).
Fig. 4 ( A ) The relative value of CCK-8 in BV2 cells after transfection with H2O2 or/and pcDNA3.1-SPRED1 for 24 h. ( B ) Flow cytometer was used to detect the fluorescence intensity of PI-labeled and FITC-labeled after transfection. ( C ) According to streaming results, the average fluorescence intensity of each group was counted. ( D, E ) Detection of the levels of Bax and Bcl2 proteins in BV2 cells after transfection, quantified in the right (E). GAPDH was used as a loading control. (*, p <0.05).CC-BY-NC-ND · From the PDF

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Cites

Research works printed but not linked: 25

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Metadata

Authors
Wei, L., Li, X., Wei, Q., Chen, L., Xu, L., & Zhou, P.
Venue
Neuroscience
Publisher
Elsevier BV
Published
2023-08
Volume
526
Pages
13-20
ISSN
0306-4522
DOI
10.1016/j.neuroscience.2023.06.012
Cited by
3
References
1
Language
en
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