Differential anti-inflammatory effects of phenolic compounds from extra virgin olive oil identified in human whole blood cultures
Abstract
Objective The olive oil-rich Mediterranean diet protects against cardiovascular disease, which involves inflammatory processes. This study investigated the effects of phenolic compounds found in extra virgin olive oil on inflammatory mediator production by human mononuclear cells.Methods Diluted human blood cultures were stimulated with lipopolysaccharide in the presence of phenolics (vanillic, p-coumaric, syringic, homovanillic and caffeic acids, kaempferol, oleuropein glycoside, and tyrosol) at concentrations of 10(-7) to 10(-4) M. Concentrations of the inflammatory cytokines tumor necrosis factor-alpha, interleukin-1beta, and interleukin-6 and of the inflammatory eicosanoid prostaglandin E2 were measured by enzyme-linked immunosorbent assay.Results Oleuropein glycoside and caffeic acid decreased the concentration of interleukin-1beta. At a concentration of 10(-4) M, oleuropein glycoside inhibited interleukin-1beta production by 80%, whereas caffeic acid inhibited production by 40%. Kaempferol decreased the concentration of prostaglandin E2. At a concentration of 10(-4) M, kaempferol inhibited prostaglandin E2 production by 95%. No effects were seen on concentrations of interleukin-6 or tumor necrosis factor-alpha and there were no effects of the other phenolic compounds.Conclusions Some, but not all, phenolic compounds derived from extra virgin olive oil decrease inflammatory mediator production by human whole blood cultures. This may contribute to the antiatherogenic properties ascribed to extra virgin olive oil.
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Responses to olive oil polyphenols were inversely correlated to dose.
“Interestingly, responses were inversely correlated to the dose (144).”
From Immunosenescence and Its Hallmarks: How to Oppose Aging Strategically? A Review of Potential Options for Therapeutic Intervention (2019) · cites this paper as [144]
In vitro studies have shown that administration of olive oil polyphenols (caffeic acid and oleuropein glycoside) to human whole blood cultures stimulated with lipopolysaccharides significantly reduced IL-1β levels compared with stimulated control cultures not incubated with olive oil polyphenols.
“In vitro studies have shown that administration of olive oil polyphenols (caffeic acid and oleuropein glycoside) to human whole blood cultures stimulated with lipopolysaccharides significantly reduced IL-1β levels compared with stimulated control cultures that were not incubated with olive oil polyphenols.”
From Immunosenescence and Its Hallmarks: How to Oppose Aging Strategically? A Review of Potential Options for Therapeutic Intervention (2019) · cites this paper as [144]
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References 29 · Cited by 223
- Authors
- Miles, E. A., Zoubouli, P., & Calder, P. C.
- Affiliations
- University of Southampton (GB)
- Corresponding author
- Miles, Elizabeth A.
- Venue
- Nutrition
- Publisher
- Elsevier BV
- Published
- 2005-03
- Volume
- 21
- Issue
- 3
- Pages
- 389-394
- ISSN
- 0899-9007 (ISSN-L)
- OpenAlex
- W1992068670
- Cited by
- 223
- References
- 29
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 7 · 2023: 8 · 2022: 8 · 2021: 14 · 2020: 9 · 2019: 9 · 2018: 8 · 2017: 12 · 2016: 17 · 2015: 14 · 2014: 15 · 2013: 8 · 2012: 16
- FWCI
- 8.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nutrition (publishedVersion)
- PubMed (publishedVersion)
- ePrints Soton (University of Southampton) (submittedVersion)
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Keywords
- extra virgin olive oil, phenolic compounds, anti-inflammatory activity, caffeic acid, kaempferol, IL-1β, prostaglandin E2, TNF-α, interleukin-6, lipopolysaccharide stimulation, mononuclear cells, Mediterranean diet, cardiovascular disease, atherosclerosis
- MeSH terms
- Olive Oil, Adolescent, Adult, Anti-Inflammatory Agents, Caffeic Acids, Enzyme-Linked Immunosorbent Assay, Humans, Interleukin-1, Male, Phenols, Plant Oils, Reference Values, Tumor Necrosis Factor-alpha, Dinoprostone, Interleukin-6, Statistics, Nonparametric, Cell Culture Techniques
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