Temporary Reduction of Membrane CD4 with the Antioxidant MnTBAP Is Sufficient to Prevent Immune Responses Induced by Gene Transfer

Da Rocha, Sylvie; Bigot, Jérémy; Onodi, Fanny; Cosette, Jérémie; Corre, Guillaume; Poupiot, Jérôme; Fenard, David; Gjata, Bernard; Galy, Anne; Neildez-Nguyen, Thi My Anh

doi:10.1016/j.omtm.2019.06.011

Temporary Reduction of Membrane CD4 with the Antioxidant MnTBAP Is Sufficient to Prevent Immune Responses Induced by Gene Transfer

Unexpectedly, the synthetic antioxidant MnTBAP was found to cause a rapid and reversible downregulation of CD4 on T cells in vitro and in vivo. This effect resulted from the internalization of membrane CD4 T cell molecules into clathrin-coated pits and involved disruption of the CD4/p56Lck comple…