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Cited by 23MicroRNA in disease regulationMechanisms of cancer metastasisCancer-related molecular mechanisms research

Sp1 Suppresses miR-3178 to Promote the Metastasis Invasion Cascade via Upregulation of TRIOBP

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Wang, Hui, Li, Kai, Mei, Yu, Huang, Xuemei, Li, Zhenglin, Yang, Qingzhu, Yang, Huanjie·2018·Molecular Therapy Nucleic Acids
DOI 10.1016/j.omtn.2018.04.008PMC PMC6023786
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Abstract

Specificity protein (Sp1) plays an important role in invasion-metastasis cascade. Sp1 regulation on protein coding genes has been extensively investigated; however, little is known about its regulation on protein non-coding genes. In this study, miR-3178 is reported as a novel target of Sp1 in multiple cancer cell models. Sp1 functions as its transcriptional suppressor as evidenced by luciferase reporter and chromatin immunoprecipitation (ChIP) assays. In line with the pro-metastatic role of Sp1, miR-3178 exerts anti-metastasis function. Overexpression of miR-3178 inhibits both migration and invasion of highly metastatic prostate, lung, and breast cancer cells whereas antagonizing miR-3178 promotes those events in their lowly metastatic counterparts. The in vivo study demonstrates that miR-3178 suppresses the tail vein inoculated prostate cancer cells to form colonies in lung, lymph node, and liver of BALB/c nude mice. miR-3178 directly targets the 3' UTR of TRIOBP-1 and TRIOBP-5, two isoforms of TRIOBP expressed in prostate, lung, and breast cancer cells. Overexpression of TRIOBP-1 could rescue miR-3178 inhibition on cell migration and invasion. Collectively, our findings reveal the regulatory axis of Sp1/miR-3178/TRIOBP in metastasis cascade. Our results suggest miR-3178 as a promising application to suppress metastasis in Sp1-overexpressed cancers.

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  • Bortezomib induces downregulation of miR-33b-3p.

    “downregulation of miR-33b-3p was induced by bortezomib, the clinically used proteasome inhibitor”

    From miR-33b-3p Acts as a Tumor Suppressor by Targeting DOCK4 in Prostate Cancer (2021) · cites this paper as [14]

    The Materials and Methods (Microarray; page unavailable) describe bortezomib treatment, and the Results (Sp1 Is a Transcriptional Suppressor of miR-3178; page unavailable) report that treatment downregulated SP1 and upregulated miR-3178. Searches for miR-33b-3p and bortezomib-induced miRNA downregulation found no passage stating what happened to miR-33b-3p. (LLM, conf=0.76)

    Experimental — accuracy not yet measured

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Cited by

Sorted by citations

Authors
Wang, H., Li, K., Mei, Y., Huang, X., Li, Z., Yang, Q., & Yang, H.
Affiliations
Harbin Institute of Technology (CN)
Corresponding author
Yang, Huanjie
ORCID
Venue
Molecular Therapy Nucleic Acids
Publisher
Elsevier BV
Published
2018-09
Volume
12
Pages
1-11
ISSN
2162-2531 (ISSN-L)
DOI
10.1016/j.omtn.2018.04.008
PMC
PMC6023786
OpenAlex
W2802571217
Cited by
23
References
1
Citations by year
2025: 3 · 2023: 1 · 2022: 3 · 2021: 6 · 2020: 8 · 2019: 2
FWCI
1.21
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecular Therapy — Nucleic Acids (publishedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Harbin Science and Technology Bureau (2017RAQXJ182)
  • Heilongjiang Postdoctoral Science Foundation (LBH-Z16072)
  • China Postdoctoral Science Foundation (2017M610201)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mechanisms of cancer metastasis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
Keywords
Sp1, TRIOBP, metastasis, cell invasion, cell migration, microRNA regulation, transcriptional regulation, prostate cancer, lung cancer, breast cancer, 3' UTR target, metastasis suppression, protein isoforms, tumor xenografts

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