CoChat
Sign Up
Cited by 8

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Isolation and partial characterization of an immunogenic antigen of Giardia lamblia

Quintero, Jael, Valdez, Alejandra, Samaniego, Brenda, Lopez-Romero, Gloria, Astiazaran-Garcia, Humberto, Rascon, Lucila, Breci, Linda, Garibay-Escobar, Adriana, Robles-Zepeda, Ramón, Velazquez, Carlos·2017·Parasitology International
DOI 10.1016/j.parint.2017.01.007
Open this paper in CoChat — free account

Abstract

Humoral and cellular immune responses play an important role during Giardia lamblia infection. Several Giardia proteins have been identified as immunogenic antigens based on their elicited humoral immune response. Poorly is known about Giardia antigens that stimulate a cellular immune response. The main purpose of this study was to isolate and partial characterize an immunogenic antigen (5G8) of G. lamblia. The 5G8 protein was isolated from G. lamblia trophozoite lysates by affinity chromatography using moAb 5G8-coupled CNBr-Sepharose. The isolated protein was analysed by electrospray tandem mass spectrometry (ESI-MS/MS), and by diverse bioinformatics tools (GiardiaDB, BLASTn, BLASTp and ExPASy). Additionally, several biochemical and immunological characteristics of the isolated protein were analysed. By ESI-MS/MS the amino acidic 5G8 sequence was deduced. The 5G8 antigen belongs to the VSP family proteins of G. lamblia. This protein is composed by one polypeptide chain (±71kDa). Using the algorithm SYFPHEITI, we identified candidate CD4 + T-cell epitopes from the 5G8 antigen, which can elicit cell-mediated immune responses. In this study, we have identified a G. lamblia protein that induces a strong immune response in infected mice. The biochemical and immunological characterization of the immunogenic 5G8 antigen may contribute to the rational design of a Giardia vaccine.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.parint.2017.01.007

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 39

  1. Singer, 2000, T-cell-dependent control of acute Giardia lamblia infections in mice, Infect. Immun., 68, 170, 10.1128/IAI.68.1.170-175.2000 · DOI
  2. Adam, 2001, Biology of Giardia lamblia, Clin. Microbiol. Rev., 14, 447, 10.1128/CMR.14.3.447-475.2001 · DOI
  3. Eckmann, 2003, Mucosal defences against Giardia, Parasite Immunol., 25, 259, 10.1046/j.1365-3024.2003.00634.x · DOI
  4. Lujan, 2006, Giardia and giardiasis, Medicina (Buenos Aires), 66, 70
  5. Lopez-Romero, 2015, Host defences against Giardia lamblia, Parasite Immunol., 8, 394, 10.1111/pim.12210 · DOI
  6. Nash, 1992, Surface antigen variability and variation in Giardia lamblia, Parasitol. Today, 7, 229, 10.1016/0169-4758(92)90119-M · DOI
  7. Langford, 2002, Central importance of immunoglobulin A in host defense against Giardia spp, Infect. Immun., 70, 11, 10.1128/IAI.70.1.11-18.2002 · DOI
  8. Tellez, 2003, Antibodies in mother's milk protect children against giardiasis, Scand. J. Infect. Dis., 35, 322, 10.1080/00365540310008041 · DOI
  9. Davids, 2006, Polymeric immunoglobulin receptor in intestinal immune defense against the lumen-dwelling protozoan parasite Giardia, J. Immunol., 177, 6281, 10.4049/jimmunol.177.9.6281 · DOI
  10. El-Gebaly, 2012, Saliva and sera IgA and IgG in Egyptian Giardia-infected children, Parasitol. Res., 111, 571, 10.1007/s00436-012-2869-y · DOI
  11. Heyworth, 1986, Antibody response to Giardia muris trophozoites in mouse intestine, Infect. Immun., 52, 568, 10.1128/iai.52.2.568-571.1986 · DOI
  12. Heyworth, 1994, Giardia muris trophozoite antigenic targets for mouse intestinal IgA antibody, J. Infect. Dis., 169, 395, 10.1093/infdis/169.2.395 · DOI
  13. Roxström-Lindquist, 2006, Giardia immunity–an update, Trends Parasitol., 22, 26, 10.1016/j.pt.2005.11.005 · DOI
  14. Khader, 2009, Th17 cells at the crossroads of innate and adaptive immunity against infectious diseases at the mucosa, Mucosal Immunol., 2, 403, 10.1038/mi.2009.100 · DOI
  15. Dreesen, 2014, Giardia muris infection in mice is associated with a protective interleukin 17A response and induction of peroxisome proliferator-activated receptor alpha, Infect. Immun., 82, 3333, 10.1128/IAI.01536-14 · DOI
  16. Cox, 2014, Giardia duodenalis stimulates partial maturation of bovine dendritic cells associated with altered cytokine secretion and induction of T-cell proliferation, Parasite Immunol., 36, 157, 10.1111/pim.12095 · DOI
  17. Velazquez, 2005, Giardia lamblia infection induces different secretory and systemic antibody responses in mice, Parasite Immunol., 27, 351, 10.1111/j.1365-3024.2005.00793.x · DOI
  18. Astiazaran-Garcia, 2009, Identification of T-cell stimulating antigens from Giardia lamblia by using Giardia-specific T-cell hybridomas, Parasite Immunol., 31, 132, 10.1111/j.1365-3024.2008.01083.x · DOI
  19. Quintero, 2013, Identification of an immunogenic protein of Giardia lamblia using monoclonal antibodies generated from infected mice, Mem. Inst. Oswaldo Cruz, 108, 616, 10.1590/0074-0276108052013013 · DOI
  20. Keister, 1983, Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile, Trans. R. Soc. Trop. Med. Hyg., 77, 487, 10.1016/0035-9203(83)90120-7 · DOI
  21. Gottstein, 1991, Antigenic variation in Giardia lamblia: infection of congenitally athymic nude and scid mice, Parasite Immunol., 16, 649, 10.1111/j.1365-3024.1991.tb00560.x · DOI
  22. Kanagawa, 2002, Regulation of diabetes development by regulatory T cells in pancreatic islet antigen-specific TCR transgenic nonobese diabetic mice, J. Immunol., 168, 6159, 10.4049/jimmunol.168.12.6159 · DOI
  23. Aurrecoechea, 2009, Integrated genomic resources for the eukaryotic protist pathogens Giardia lamblia and Trichomonas vaginalis, Nucleic Acids Res., 37, 526, 10.1093/nar/gkn631 · DOI
  24. Artimo, 2012, ExPASy: SIB bioinformatics resource portal, Nucleic Acids Res., 40, 597, 10.1093/nar/gks400 · DOI · Paper page
  25. Nash, 2002, Surface antigenic variation in Giardia lamblia, Mol. Microbiol., 45, 585, 10.1046/j.1365-2958.2002.03029.x · DOI
  26. Rammensee, 1999, SYFPEITHI: database for MHC ligands and peptide motifs, Immunogenetics, 50, 213, 10.1007/s002510050595 · DOI
  27. Wielinga, 2015, Identification of polymorphic genes for use in assemblage B genotyping assays through comparative genomics of multiple assemblage B Giardia duodenalis isolates, Mol. Biochem. Parasitol., 201, 1, 10.1016/j.molbiopara.2015.05.002 · DOI
  28. Adam, 2013, Genome sequencing of Giardia lamblia genotypes A2 and B isolates (DH and GS) and comparative analysis with the genomes of genotypes A1 and E (WB and Pig), Genome Biol. Evol., 5, 2498, 10.1093/gbe/evt197 · DOI
  29. Gillin, 1990, Isolation and expression of the gene for a major surface protein of Giardia lamblia, Proc. Natl. Acad. Sci. U. S. A., 87, 4463, 10.1073/pnas.87.12.4463 · DOI
  30. Jerlström-Hultqvist, 2010, Genome analysis and comparative genomics of a Giardia intestinalis assemblage E isolate, BMC Genomics, 7, 543, 10.1186/1471-2164-11-543 · DOI
  31. Velazquez, 2002, Chemical identification of a low abundance lysozyme peptide family bound to I-Ak histocompatibility molecules, J. Biol. Chem., 277, 42514, 10.1074/jbc.M202316200 · DOI
  32. Nelson, 1992, Identification of the naturally processed form of hen egg white lysozyme bound to the murine major histocompatibility, Proc. Natl. Acad. Sci. U. S. A., 89, 7380, 10.1073/pnas.89.16.7380 · DOI
  33. Gillin, 1996, Cell biology of the primitive eukaryote Giardia lamblia, Annu. Rev. Microbiol., 50, 679, 10.1146/annurev.micro.50.1.679 · DOI
  34. Gargantini, 2016, Antigenic variation in the intestinal parasite Giardia lamblia, Curr. Opin. Microbiol., 32, 52, 10.1016/j.mib.2016.04.017 · DOI
  35. Touz, 2008, Arginine deminase has multiple regulatory roles in the biology of Giardia lamblia, J. Cell Sci., 121, 2930, 10.1242/jcs.026963 · DOI
  36. Lujan, 2008, Antigenic variation in Giardia lamblia is regulated by RNA interference, Nature, 456, 750, 10.1038/nature07585 · DOI
  37. Adam, 2010, The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution, BMC Genomics, 11, 10.1186/1471-2164-11-424 · DOI
  38. Nash, 1991, Isolate and epitope variability in susceptibility of Giardia lamblia to intestinal proteases, Infect. Immun., 59, 1334, 10.1128/iai.59.4.1334-1340.1991 · DOI
  39. Singer, 2000, The role of normal flora in Giardia lamblia infections in mice, J. Infect. Dis, 181, 1510, 10.1086/315409 · DOI

Cited by

Sorted by citations

See all 8

Metadata

Authors
Quintero, J., Valdez, A., Samaniego, B., Lopez-Romero, G., Astiazaran-Garcia, H., Rascon, L., Breci, L., Garibay-Escobar, A., Robles-Zepeda, R., & Velazquez, C.
Venue
Parasitology International
Publisher
Elsevier BV
Published
2017-06
Volume
66
Issue
3
Pages
324-330
ISSN
1383-5769
DOI
10.1016/j.parint.2017.01.007
Cited by
8
References
1
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Funders
National Council for Science and Technology of Mexico (CB-2010-01/155224)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review