The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Expression of the Multidrug Resistance P-Glycoprotein, (ABCB1 glycoprotein) in the Human Placenta Decreases with Advancing Gestation
Abstract
The multidrug resistance p-glycoprotein (P-gp), encoded by the ABCB1 gene, is a plasma membrane protein that actively extrudes a wide variety of substances from cells. Preliminary studies in mice have shown that the ABCB1/P-gp can protect the fetus from a number of toxic substances. ABCB1/P-gp is expressed in the human placenta and is potentially capable of protecting the fetus from a large number of drugs and toxins, including herbicides and pesticides. The protein can also extrude various steroids including certain glucocorticoids and may therefore play an important role in regulating fetal access of glucocorticoids. The aim of the present study was to examine the expression profile and cellular localization of ABCB1/P-gp in human placenta throughout gestation. We hypothesized that there would be gestational age-related changes in the expression of the protein. ABCB1/P-gp mRNA was measured by Real-Time PCR using specific probes in tissues obtained from 6 weeks gestation to term. ABCB1/P-gp mRNA levels in placental tissue obtained at 6-10 weeks (n=5) and 24-35 weeks (n=5) were significantly higher than in tissues obtained at term (38-41 weeks gestation) by elective C-section (n=6) or following labor (n=6). The profile of ABCB1/P-gp protein levels, quantified using Western analysis, demonstrated a similar decrease with advancing gestation. At all gestational ages ABCB1/P-gp was localized by immunohistochemistry to the syncytiotrophoblast. In term tissues, it appeared to be localized to some areas of the villi and not others. Together, these data indicate that with advancing gestation there is a decrease in the level of ABCB1/P-gp in the human placenta indicating that the fetus may be more susceptible to toxic insults in the latter part of gestation. Further, the reduction in ABCB1/P-gp expression may contribute to the increased transfer of maternal cortisol to the fetus that is known to occur in late gestation.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The mRNA and protein levels of P-glycoprotein reduce across gestation, suggesting the fetus may be more exposed to toxic insults later in pregnancy (519).”
From Placental Origins of Chronic Disease (2016) · cites this paper as [519]
Citations
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants
Juliano, R. L., & Ling, V. W. T. (1976) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(76)90160-7 · Cited by 3,317
Schinkel, A. H., Smit, J., van Tellingen, O., Beijnen, J. H., Wagenaar, E., van Deemter, L., Mol, C. A. A. M., van der Valk, M. A., Robanus-Maandag, E. C., Riele, H. T., et al. (1994) · Cell · doi:10.1016/0092-8674(94)90212-7 · Cited by 2,284
Endocrine and Paracrine Regulation of Birth at Term and Preterm*
Challis, J. R. G., Matthews, S. G., Gibb, W., & Lye, S. J. (2000) · Endocrine Reviews · doi:10.1210/edrv.21.5.0407 · Cited by 995
Self-citationP-Glycoprotein, a gatekeeper in the blood–brain barrier
Schinkel, A. H. (1999) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(98)00085-4 · Cited by 963
Schinkel, A. H., Mayer, U., Wagenaar, E., Mol, C. A. A. M., van Deemter, L., Smit, J. J. M., van der Valk, M. A., Voordouw, A. C., Spits, H., van Tellingen, O., et al. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.8.4028 · Cited by 937
Human P-glycoprotein transports cortisol, aldosterone, and dexamethasone, but not progesterone.
Ueda, K., Okamura, N., Hirai, M., Tanigawara, Y., Saeki, T., Kioka, N., Komano, T., & Hori, R. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)35757-0 · Cited by 672
Cytokines, Prostaglandins and Parturition—A Review
Keelan, J. A., Blumenstein, M., Helliwell, R. J. A., Sato, T., Marvin, K. W., & Mitchell, M. D. (2003) · Placenta · doi:10.1053/plac.2002.0948 · Cited by 617
Endocrine and Paracrine Regulation of Birth at Term and Preterm
Challis, J. R. G. (2000) · Endocrine Reviews · doi:10.1210/er.21.5.514 · Cited by 609
Placental 11β‐hydroxysteroid dehydrogenase: a key regulator of fetal glucocorticoid exposure
Benediktsson, R., Calder, A. A., Edwards, C. R. W., & Seckl, J. R. (1997) · Clinical Endocrinology · doi:10.1046/j.1365-2265.1997.1230939.x · Cited by 536
Flagged first, then by citations
Cited by
Sorted by citations
Items 201–223 of 223
Placental Pharmacology---Implications for Therapy in Pregnancy
Bapat, P. (2016) · TSpace (University of Toronto)
Regulation of Placental Metabolism of Xenobiotics
Pávek, P., & Smutný, T. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-801238-3.64274-5
Carey, J. (2012) · uO Research (University of Ottawa) · doi:10.20381/ruor-5769
Hypothalamic pituitary adrenal axis dysregulation in obese pregnancy
Stirrat, L. (2018) · ERA
Drugs and medication in pregnancy
Feghali, M. N., & Caritis, S. N. (2018) · Wiley eBooks · doi:10.1002/9781119072980.ch23
PXR-mediated Regulation of Placental Drug Transporters Impact on Fetal Exposure to Lopinavir
Gahir, S. (2014) · TSpace (University of Toronto)
Imprinted Gene Regulation in the Placenta and Fetal Development
Shuai, W. S. (2020) · Biomedical Journal of Scientific & Technical Research · doi:10.26717/bjstr.2020.28.004645
Methionine-Mediated Protection of Fetal Programming Induced by Synthetic Glucocorticoid Treatment
Mouratidis, A. (2018) · TSpace (University of Toronto)
Scott, H. (2021) · Carleton University's Institutional Repository (MacOdrum Library, Carleton University) · doi:10.22215/etd/2021-14714
Drug therapy in pregnant and nursing women
Stika, C. S., & Frederiksen, M. C. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-819869-8.00009-4
ABC Transporters: Individual-Specific Considerations
Koehn, L. M. (2021) · Springer eBooks · doi:10.1007/978-3-030-51519-5_81-1
Carrillo, I. O., Peñafiel, C. O. R., Mendoza-Salas, I., Maldonado, R. C., Laguna, A. I. G., la Cruz-Rosas, A. D., Murillo, C. M., & Tovar, A. M. (2022) · Infection and Drug Resistance · doi:10.2147/idr.s358389
Hernández, M., Cohen, J. M., Skåra, K. H., Grindstad, T., Lee, Y., Magnus, P. M., Njølstad, P. R., Andreassen, O. A., Corfield, E. C., Havdahl, A., et al. (2023) · medRxiv · doi:10.1101/2023.09.13.23295417
Drug Transport Across the Placental Barrier
Bryant, V. F., Shah, M. K., Waltz, J., & Rytting, E. (2023) · doi:10.1002/9781119660699.ch8
Hernández, M., Cohen, J. M., Skåra, K. H., Grindstad, T., Lee, Y., Magnus, P. M., Njølstad, P. R., Andreassen, O. A., Corfield, E. C., Havdahl, A., et al. (2024) · iScience · doi:10.1016/j.isci.2024.109285
Regulation of Placental Metabolism of Xenobiotics
Pávek, P., & Smutný, T. (2018) · Elsevier eBooks · doi:10.1016/b978-0-323-95488-4.11009-5
Prenatal Cannabis and Tobacco: Studies in Animal Models
Edenfield, R. C., D'Mello, R. J., Shorey‐Kendrick, L. E., Crosland, A., Hagen, O. L., McEvoy, C. T., Spindel, E. R., Murphy, S. K., Lo, J. O.-T., & Wetendorf, M. (2026) · Advances in experimental medicine and biology · doi:10.1007/978-3-032-12741-9_9 · Cited by 1
Mahdi, A., Abduljalil, K., Moreira, F. D. L., Mehta, H. R., Benzi, J. R. D. L., Marcolin, A. C., Schmidt, S., & de Moraes, N. V. (2026) · The Journal of Clinical Pharmacology · doi:10.1002/jcph.70308
Metadata
- Authors
- Sun, M., Kingdom, J., Baczyk, D., Lye, S. J., Matthews, S. G., & Gibb, W.
- Affiliations
- University of Ottawa (CA)
- Mount Sinai Hospital (CA)
- Corresponding author
- Gibb, W.
- Venue
- Placenta
- Publisher
- Elsevier BV
- Published
- 2006-06
- Volume
- 27
- Issue
- 6-7
- Pages
- 602-609
- ISSN
- 0143-4004 (ISSN-L)
- OpenAlex
- W2021031411
- Cited by
- 223
- References
- 38
- Citations by year
- 2026: 4 · 2024: 3 · 2023: 9 · 2022: 16 · 2021: 14 · 2020: 13 · 2019: 11 · 2018: 16 · 2017: 10 · 2016: 8 · 2015: 13 · 2014: 12 · 2013: 20 · 2012: 17
- FWCI
- 3.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Placenta (publishedVersion)
- PubMed (publishedVersion)
- UWA Profiles and Research Repository (UWA) (submittedVersion)
- Funders
- Canadian Institutes of Health Research
- Topics
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Pregnancy and Medication Impact
- Keywords
- P-glycoprotein, multidrug resistance protein, human placenta, gestational age, syncytiotrophoblast
- MeSH terms
- Adult, Female, Gestational Age, Humans, Maternal-Fetal Exchange, Placenta, Pregnancy, RNA, Messenger, Blotting, Western, Drug Resistance, Multiple, Gene Expression Regulation, Developmental, Reverse Transcriptase Polymerase Chain Reaction, ATP Binding Cassette Transporter, Subfamily B, Member 1
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
