Recombinant Fab expression and secretion in Escherichia coli continuous culture at medium cell densities: Influence of temperature
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Several studies have demonstrated that yield of recombinant antibody fragments in E. coli significantly improves at growth temperature below 30 °C, likely due to reduction of translation rate that favors proper Ig-like folding and reduces aggregation.
“Several studies have demonstrated that the yield of recombinant antibody fragments in E. coli significantly improves at growth temperature below 30 °C, likely due to the reduction of translation rate that favors proper Ig-like folding and reduces aggregation [90,91,92].”
From Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments (2020) · cites this paper as [92]
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Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments
Sandomenico, A., Sivaccumar, J. P., & Ruvo, M. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21176324 · Cited by 134 · see citing works
- Authors
- Rodríguez-Carmona, E., Cano-Garrido, O., Dragosits, M., Maurer, M., Mader, A., Kunert, R., Mattanovich, D., Villaverde, A., & Vázquez, F.
- Affiliations
- BOKU University (AT)
- Hochschule Campus Wien (AT)
- Universitat Autònoma de Barcelona (ES)
- Corresponding author
- Vázquez, Felícitas
- Venue
- Process Biochemistry
- Publisher
- Elsevier BV
- Published
- 2012-03
- Volume
- 47
- Issue
- 3
- Pages
- 446-452
- ISSN
- 0032-9592 (ISSN-L)
- 1359-5113
- OpenAlex
- W2070597143
- Cited by
- 31
- References
- 0
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 1 · 2023: 1 · 2021: 6 · 2020: 5 · 2018: 3 · 2017: 1 · 2016: 2 · 2014: 3 · 2013: 4
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Process Biochemistry (publishedVersion)
- Funders
- European Science Foundation
- European Commission
- 6th Framework Programme
- Austrian Science Fund
- The Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN, Spain)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Escherichia coli, continuous culture, growth temperature, periplasmic secretion, plasmid loss, biomass yield, product yield
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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