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Cited by 44Ovarian function and disordersReproductive Biology and FertilityGenetic and Kidney Cyst Diseases

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Luteinizing hormone/chorionic gonadotrophin receptor overexpressed in granulosa cells from polycystic ovary syndrome ovaries is functionally active

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Kanamarlapudi, Venkateswarlu, Gordon, Uma D., López Bernal, Andrés·2016·Reproductive BioMedicine Online
DOI 10.1016/j.rbmo.2016.03.003
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Abstract

Polycystic ovarian syndrome (PCOS) is associated with anovulatory infertility. Luteinizing hormone/chorionic gonadotrophin receptor (LHCGR), which is critical for ovulation, has been suggested to be expressed prematurely in the ovarian follicles of women with PCOS. This study aimed to analyse the expression and activity of LHCGR in ovarian granulosa cells from PCOS patients and the involvement of ARF6 small GTPase in LHCGR internalization. Granulosa cells (GC) isolated from follicular fluid collected during oocyte retrieval from normal women (n = 19) and women with PCOS (n = 17) were used to study differences in LHCGR protein expression and activity between normal and PCOS patients. LHCGR expression is up-regulated in GC from PCOS women. LHCGR in PCOS GC is functionally active, as shown by increased cAMP production upon human gonadotrophin (HCG)-stimulation. Moreover, ARF6 is highly expressed in GC from PCOS patients and HCG-stimulation increases the concentrations of active ARF6. The inhibition of ARF6 activation attenuates HCG-induced LHCGR internalization in both normal and PCOS GC, indicating that there are no alterations in LHCGR internalisation in GC from PCOS. In conclusion, the expression and activation of LHCGR and ARF6 are up-regulated in GC from PCOS women but the mechanism of agonist-induced LHCGR internalization is unaltered.

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  • Granulosa cells from small antral follicles of women with PCOS prematurely switch from FSH to LH responsiveness, indicated by elevated LH receptor expression and reduced FSH receptor expression compared with unaffected women.

    “The granulosa cells from small antral follicles of women with PCOS prematurely switch from FSH to LH responsiveness, as indicated by elevated LH receptor expression and reduced FSH receptor expression compared with unaffected women (Franks & Hardy, 2020; Kanamarlapudi et al., 2016; Owens et al., 2019).”

    From Endometriosis and polycystic ovary syndrome are diametric disorders (2021) · cites this paper as Kanamarlapudi et al., 2016

    The indexed title identifies overexpressed, functionally active LH receptor in granulosa cells from PCOS ovaries. The available passages do not establish that the cells came from small antral follicles, that FSH receptor expression was reduced, or that responsiveness switched prematurely from FSH to LH. (LLM, conf=0.78)

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Cited by

Sorted by citations

  • Endometriosis and polycystic ovary syndrome are diametric disorders

    Dinsdale, N. L., & Crespi, B. J. (2021) · Evolutionary Applications · doi:10.1111/eva.13244 · Cited by 90

    “…granulosa cells from small antral follicles of women with PCOS prematurely switch from FSH to LH responsiveness, as indicated by elevated LH receptor expression and reduced FSH receptor expression compared with unaffected women (Franks & Hardy, 2020; Kanamarlapudi et al., 2016; Owens et al., 2019).”
    Background

Metadata

Authors
Kanamarlapudi, V., Gordon, U. D., & López Bernal, A.
Affiliations
  • Swansea University (GB)
  • North Bristol NHS Trust (GB)
Corresponding author
Kanamarlapudi, Venkateswarlu
ORCID
Kanamarlapudi, Venkateswarlu
Venue
Reproductive BioMedicine Online
Publisher
Elsevier BV
Published
2016-06
Volume
32
Issue
6
Pages
635-641
ISSN
1472-6483 (ISSN-L)
DOI
10.1016/j.rbmo.2016.03.003
OpenAlex
W2329133225
Cited by
44
References
2
Citations by year
2026: 3 · 2025: 4 · 2024: 6 · 2023: 5 · 2022: 5 · 2021: 8 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 3 · 2016: 1
FWCI
4.43
Type
journal article
Language
en
Open access
Licence
Locations
  • Reproductive BioMedicine Online (publishedVersion)
  • PubMed (publishedVersion)
  • Explore Bristol Research (publishedVersion, cc-by-nc-nd)
  • Bristol Research (University of Bristol) (publishedVersion, cc-by-nc-nd)
Funders
  • BBSRC (BB/C515455/2)
  • MRC (G0401232)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
  • Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
polycystic ovary syndrome, granulosa cells, ARF6, receptor internalization, cAMP production, human chorionic gonadotropin, ovulation, anovulatory infertility, receptor overexpression
MeSH terms
ADP-Ribosylation Factor 6, Cyclic AMP, Female, Chorionic Gonadotropin, Ovarian Follicle, Granulosa Cells, Humans, Luteinizing Hormone, Ovary, Ovulation, Polycystic Ovary Syndrome, Receptors, LH, Time Factors, Lutein, Follicular Fluid, GTP Phosphohydrolases, ADP-Ribosylation Factors

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