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Zebrafish embryos as an alternative to animal experiments—A commentary on the definition of the onset of protected life stages in animal welfare regulations

Strähle, Uwe, Scholz, Stefan, Geisler, Robert, Greiner, Petra, Hollert, Henner, Rastegar, Sepand, Schumacher, Axel, Selderslaghs, Ingrid, Weiss, Carsten, Witters, Hilda, Braunbeck, Thomas·2012·Reproductive Toxicology
DOI 10.1016/j.reprotox.2011.06.121
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Abstract

Worldwide, the zebrafish has become a popular model for biomedical research and (eco)toxicology. Particularly the use of embryos is receiving increasing attention, since they are considered as replacement method for animal experiments. Zebrafish embryos allow the analysis of multiple endpoints ranging from acute and developmental toxicity determination to complex functional genetic and physiological analysis. Particularly the more complex endpoints require the use of post-hatched eleutheroembryo stages. According to the new EU Directive 2010/63/EU on the protection of animals used for scientific purposes, the earliest life-stages of animals are not defined as protected and, therefore, do not fall into the regulatory frameworks dealing with animal experimentation. Independent feeding is considered as the stage from which free-living larvae are subject to regulations for animal experimentation. However, despite this seemingly clear definition, large variations exist in the interpretation of this criterion by national and regional authorities. Since some assays require the use of post-hatched stages up to 120 h post fertilization, the literature and available data are reviewed in order to evaluate if this stage could still be considered as non-protected according to the regulatory criterion of independent feeding. Based on our analysis and by including criteria such as yolk consumption, feeding and swimming behavior, we conclude that zebrafish larvae can indeed be regarded as in…

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Metadata

Authors
Strähle, U., Scholz, S., Geisler, R., Greiner, P., Hollert, H., Rastegar, S., Schumacher, A., Selderslaghs, I., Weiss, C., Witters, H., & Braunbeck, T.
Venue
Reproductive Toxicology
Publisher
Elsevier BV
Published
2012-04
Volume
33
Issue
2
Pages
128-132
ISSN
0890-6238
DOI
10.1016/j.reprotox.2011.06.121
Cited by
673
References
0
Language
en
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CoChat. (2026). Citation check: Zebrafish embryos as an alternative to animal experiments—A commentary on the definition of the onset of protected life stages in animal welfare regulations. https://app.cochat.ai/papers/10.1016/j.reprotox.2011.06.121
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Strähle, U., Scholz, S., Geisler, R., Greiner, P., Hollert, H., Rastegar, S., Schumacher, A., Selderslaghs, I., Weiss, C., Witters, H., & Braunbeck, T. (2012). Zebrafish embryos as an alternative to animal experiments—A commentary on the definition of the onset of protected life stages in animal welfare regulations. Reproductive Toxicology. https://doi.org/10.1016/j.reprotox.2011.06.121