Leishmanicidal activity of polyphenolic-rich extract from husk fiber of Cocos nucifera Linn. (Palmae)
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The available therapy for leishmaniasis, which affects 2 million people per annum, still causes serious side effects. The polyphenolic-rich extract from the husk fiber of Cocos nucifera Linn. (Palmae) presents antibacterial and antiviral activities, also inhibiting lymphocyte proliferation, as shown by our group in previous works. In the present study, the in vitro leishmanicidal effects of C. nucifera on Leishmania amazonensis were evaluated. The minimal inhibitory concentration of the polyphenolic-rich extract from C. nucifera to completely abrogate parasite growth was 10 microg/ml. Pretreatment of peritoneal mouse macrophages with 10 microg/ml of C. nucifera polyphenolic-rich extract reduced approximately 44% the association index between these macrophages and L. amazonensis promastigotes, with a concomitant increase of 182% in nitric oxide production by the infected macrophage in comparison to nontreated macrophages. These results provide new perspectives on drug development against leishmaniasis, since the extract of C. nucifera at 10 microg/ml is a strikingly potent leishmanicidal substance which inhibited the growth of both promastigote and amastigote developmental stages of L. amazonensis after 60 min, presenting no in vivo allergenic reactions or in vitro cytotoxic effects in mammalian systems.
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The lyophilized extract and fractions, as well as ethyl acetate extracts, from the C. nucifera fiber are rich in polyphenols, compounds such as catechins, epicatechins, tannins, and flavonoids.
“The lyophilized extract and fractions, as well as ethyl acetate extracts, from the C. nucifera fiber are rich in polyphenols, compounds such as catechins, epicatechins, tannins, and flavonoids (7,33–35).”
From Cocos nucifera (L.) (Arecaceae): A phytochemical and pharmacological review (2015) · cites this paper as [33]
Pretreatment of mouse peritoneal macrophages with 10 μg/mL of C. nucifera polyphenolic-rich extract reduced by approximately 44% their rate of association with L. amazonensis promastigotes with a simultaneous increase of 182% in nitric oxide production by macrophages compared with untreated macrophages.
“In addition, pretreatment of mouse peritoneal macrophages with 10 μg/mL of C. nucifera polyphenolic-rich extract reduced by approximately 44% their rate of association with L. amazonensispromastigotes with a simultaneous increase of 182% in nitric oxide production by macrophages compared with untreated macrophages.”
From Cocos nucifera (L.) (Arecaceae): A phytochemical and pharmacological review (2015) · cites this paper as [33]
The polyphenolic-rich extract obtained from coconut husk fiber completely inhibited the cellular growth of L. amazonensis promastigote forms (MIC 10 μg/mL) and killed 100% of both developmental stages of the parasite after 60 min (at 10 and 20 μg/mL).
“The polyphenolic-rich extract obtained from coconut husk fiber completely inhibited the cellular growth of L. amazonensis promastigote forms (MIC 10 μg/mL) and killed 100% of both developmental stages of the parasite after 60 min (at 10 and 20 μg/mL).”
From Cocos nucifera (L.) (Arecaceae): A phytochemical and pharmacological review (2015) · cites this paper as [33]
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References 38 · Cited by 115
- Authors
- Mendonça-Filho, R. R., Rodrigues, I. A., Alviano, D. S., Santos, A. L. S., Soares, R. M. A., Alviano, C. S., Lopes, A. H. C. S., & do Socorro S. Rosa, M.
- Affiliations
- Universidade Federal do Rio de Janeiro (BR)
- Corresponding author
- Mendonça-Filho, Ricardo R.
- Venue
- Research in Microbiology
- Publisher
- Elsevier BV
- Published
- 2004-04
- Volume
- 155
- Issue
- 3
- Pages
- 136-143
- ISSN
- 0769-2609 (ISSN-L)
- 0923-2508
- OpenAlex
- W1970963678
- Cited by
- 115
- References
- 38
- Citations by year
- 2025: 1 · 2023: 15 · 2022: 7 · 2021: 6 · 2020: 8 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 7 · 2015: 6 · 2014: 6 · 2013: 5 · 2012: 7
- FWCI
- 3.12
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Research in Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Physical Sciences › Chemistry › Inorganic Chemistry › Coconut Research and Applications
- Health Sciences › Medicine › Pharmacology › Leech Biology and Applications
- Keywords
- antileishmanial activity, Cocos nucifera, coconut husk fiber, polyphenolic extracts, Leishmania amazonensis, leishmaniasis, nitric oxide production, promastigotes, amastigotes, natural product drug development
- MeSH terms
- Animals, Cocos, Female, Flavonoids, Leishmania, Leishmaniasis, Phytotherapy, Microbial Sensitivity Tests, Nitric Oxide, Phenols, Plant Extracts, Rabbits, Macrophages, Peritoneal, Mice, Polyphenols
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